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High-activity plant lactobacillus tomato juice culture medium extracellular metabolic product, and preparation method and application thereof

A technology of tomato juice medium and extracellular metabolites, applied in the field of microbial metabolites, can solve the problems of reducing bacterial activity, unsatisfactory anti-oxidation effect, unsuitable for human consumption, etc., and achieves simple formula and good protective effect Effect

Active Publication Date: 2018-01-05
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, currently on the market, lactic acid bacteria (Lactobacillus plantarum) food (lactic acid bacteria culture medium) is added with different contents of inorganic salts and surfactants to promote its growth and avoid agglomeration and precipitation, and add preservatives to prolong the shelf life but reduce the bacteria. Activity
The effect of two strains of lactic acid bacteria on the antioxidant activity of mouse serum and liver tissue (Food Research and Development, October 2010, Volume 31, Issue 10, Huang Shanshan, etc.) found that the metabolites of Lactobacillus have an antioxidative effect on mouse serum and liver tissue However, it uses MRS medium, and its metabolites have high concentrations of inorganic salts and surfactants, which are not suitable for human consumption, and their metabolites are not ideal for antioxidant effects

Method used

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  • High-activity plant lactobacillus tomato juice culture medium extracellular metabolic product, and preparation method and application thereof
  • High-activity plant lactobacillus tomato juice culture medium extracellular metabolic product, and preparation method and application thereof
  • High-activity plant lactobacillus tomato juice culture medium extracellular metabolic product, and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The preparation of the tomato juice medium in this example is as follows: peel 500g of tomatoes, add 1000ml of water to conditioner to extract the juice, filter through 160 mesh to obtain fresh tomato juice, add 2% glucose, 1% soybean protein, and 0.5% yeast extract according to the following ratio Juice, sterilized at 115°C for 20 minutes before use.

[0060] The extracellular metabolites of Lactobacillus plantarum tomato juice medium in the present embodiment are prepared by the method comprising the following steps:

[0061] 1) The number of bacteria obtained with tomato juice medium is 10 8 The Lactobacillus plantarum suspension per ml was inoculated into the tomato juice culture with an inoculum of 1% and cultivated at 42°C for 20 hours to obtain a Lactobacillus plantarum seed solution;

[0062] 2) Inoculate the Lactobacillus plantarum seed solution in step 1) into the tomato juice medium at 42°C for 20 hours at an inoculum size of 1%, and place it in a refrigerat...

Embodiment 2

[0065] The preparation of the tomato juice medium in this example is as follows: peel 500g of tomato, add 1000ml of water to extract the juice from a conditioner, filter through 160 mesh to obtain fresh tomato juice, add 1.5% glucose, 1.5% soybean protein, and 0.7% yeast extract according to the following ratio Juice, sterilized at 115°C for 20 minutes before use.

[0066] The extracellular metabolites of Lactobacillus plantarum tomato juice medium in the present embodiment are prepared by the method comprising the following steps:

[0067] 1) The number of bacteria obtained with tomato juice medium is 10 7 The Lactobacillus plantarum suspension per ml was inoculated into the tomato juice culture with an inoculum of 1% and cultivated at 42°C for 18 hours to obtain a Lactobacillus plantarum seed solution;

[0068] 2) Inoculate the Lactobacillus plantarum seed solution in step 1) into the tomato juice medium at 42°C for 18h at an inoculum size of 1%, place it in a refrigerator ...

Embodiment 3

[0070] The preparation of the tomato juice medium in this example is as follows: peel 500g of tomato, add 1000ml of water to conditioner to extract the juice, filter through 160 mesh to obtain fresh tomato juice, add 2.5% glucose, 0.5% soybean protein, and 0.3% yeast extract according to the following ratio Juice, sterilized at 115°C for 20 minutes before use.

[0071] The extracellular metabolites of Lactobacillus plantarum tomato juice medium in the present embodiment are prepared by the method comprising the following steps:

[0072] 1) The number of bacteria obtained with tomato juice medium is 10 9 The Lactobacillus plantarum suspension per ml was inoculated into the tomato juice culture with an inoculum of 1% and cultivated at 42°C for 24 hours to obtain a Lactobacillus plantarum seed solution;

[0073] 2) Inoculate the Lactobacillus plantarum seed solution in step 1) into the tomato juice medium at 42°C for 16h at an inoculum size of 1%, put it in a refrigerator at 6°C...

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Abstract

The invention relates to a high-activity plant lactobacillus tomato juice culture medium extracellular metabolic product, and a preparation method and application thereof, and belongs to the technicalfield of application of a microbial metabolic product. During preparation of the plant lactobacillus extracellular metabolic product provided by the invention, the used culture medium formula comprises 1.5 to 2.5 percent of glucose, 0.5 to 1.5 percent of soybean protein, 0.3 to 0.7 percent of yeast dipping juice and the balance of tomato juice. The formula is simple, buffer system inorganic saltand a surfactant are not added, and the prepared metabolic product is suitable for a human body to take. Through double verification of in vitro cell and living body biological experiments, the plantlactobacillus tomato juice culture medium extracellular metabolic product has a protective effect on cellular oxidation stress injury and can improve the immunologic defense function of the body; andthrough acute alcoholic liver injury induction experiments, the tomato juice culture medium extracellular metabolic product has a good protective effect on the liver injury of animal bodies.

Description

technical field [0001] The invention relates to an extracellular metabolite of a high-activity Lactobacillus plantarum tomato juice medium, a preparation method and an application thereof, and belongs to the technical field of application of microbial metabolites. Background technique [0002] During the normal physiological activities in the body, there are oxidation reactions involving the participation of oxidative active substances. The free radical scavenging system in the human body can maintain the dynamic balance of oxygen free radicals. The dynamic balance of free radical metabolism is the basic element to maintain life and health. When the new free radicals produced exceed their own antioxidant capacity, or the excessive intake of exogenous oxidative substances will lead to excessive accumulation of oxygen free radicals in cells Oxidative stress caused by excessive free radicals is one of the important reasons for the damage and disease of various tissues and organ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04A61K36/81A61P1/00A61P1/16A61P39/00C12R1/25A61K35/747
Inventor 曾爱兵
Owner WENZHOU MEDICAL UNIV
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