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Method of utilizing mesenchymal stem cell transplantation to treat spinal cord injury

A spinal cord injury and stem cell technology, applied in the medical field, can solve problems such as hindering the regeneration of the central nervous system and neuron damage in the central nervous system, and achieve the effects of preventing demyelination, strong cell activity, and convenient access.

Inactive Publication Date: 2018-01-16
杨涛
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AI Technical Summary

Problems solved by technology

[0009] The above axonal growth inhibitory factors, coupled with insufficient supply of local blood flow, lack of neurotrophic factors in the injured area, immune response, stimulation of endocrine hormones, and damage of free radicals accelerate the damage of neurons in the central nervous system and hinder the central nervous system. Nerve regeneration

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  • Method of utilizing mesenchymal stem cell transplantation to treat spinal cord injury

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Embodiment 1

[0039] Embodiment 1: as figure 1 As shown, the present invention provides a method for treating spinal cord injury with mesenchymal stem cell transplantation, comprising the steps of:

[0040] 1) Preoperative preparation: preoperative medication: preoperative medication has sedative and analgesic effects; commonly used drugs are phenobarbital sodium 0.05-0.1g, pethidine hydrochloride 25-50mg; Intra-injection; stem cell preparation: extract 10ml of the patient's own bone marrow, after heparinization, use the density gradient cell separation medium Percoll (1.073g / ml; GE Healthcare, Piscataway, NJ, USA) to perform gradient centrifugation on the bone marrow to collect mononuclear cells, to 1.6 x 10 5 / cm 2 The inoculum density was placed in low-glucose DMEM medium (Invitrogen) containing 10% serum replacer (Serum Replacer; Invitrogen, Carlsbad, CA, USA), 1% non-essential amino acids (Invitrogen) and 1 mM L-glutamine (Invitrogen). cultured for 1 week. Cells were then digested ...

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Abstract

The invention discloses a method of utilizing mesenchymal stem cell (MSC) transplantation to treat spinal cord injury. The method comprises preparation before surgery, stem cell transplantation, and postoperative treatment. MSC transplantation can improve the axonal regeneration performance, prevent demyelination, promote remyelination and repair of spinal cord injury, and recover the neurologicalfunctions after spinal cord injury. The effects have been proved by experiments. MSC transplantation is an important part of cell transplantation for treating spinal cord injury. Achievements in manyaspects are obtained, and the research has been lifted to the same level of gene clone. Due to the advantages of MSC, the MSC has become a hot research field in academe. New hopes are brought to thepatients of spinal cord injury. At the same time, MSC resources are wide and MSC are easily available. There is no ethical limit. The multiplication capacity is strong. The cell activity is strong. The cell differentiation can be well modulated and MSC has a multi-directional differentiation potential and can be used as a good carrier for gene treatment.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a method for treating spinal cord injury with mesenchymal stem cell transplantation. Background technique [0002] Mesenchymal stem cells (Mesenchymal Stem Cells, MSC) refer to another large class of pluripotent stem cells with multi-directional differentiation potential besides hematopoietic stem cells in the body, which are derived from undifferentiated mesenchymal cells of mesoderm and are easy to isolate and culture. , multi-differentiation potential, paracrine effect and immunoregulatory functions, etc., so it is considered to be the most promising stem cell for the treatment of various diseases. MSC has become one of the important seed cells in the strategy of stem cell transplantation for the treatment of spinal cord injury because of its abundant sources, easy acquisition, strong proliferation ability, no ethical restrictions, low immune rejection, and the ability to tra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P25/00A61P19/08A61B17/00
Inventor 杨涛隋昳
Owner 杨涛
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