Full-automatic sturgeon gonad tissue puncture sampling method

A fully automatic, gonadal technology, applied in the field of histological research, can solve the problems of sampling failure and sampling failure, and achieve the effect of improving the judgment result and improving the success rate of sampling

Inactive Publication Date: 2018-01-19
WATER ENG ECOLOGICAL INST CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing methods use surgical sampling, minimally invasive technology, and manual pu

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Ovary Sampling of Sturgeon Specimens

[0017] (1) Preparation before puncture: Disinfect the puncture needle, put it into the puncture gun, adjust it, and make it in the trigger state. German automatic puncture gun, MG15-22, Bard puncture needle MN1816, the sampling length is set to 15mm.

[0018] (2) B-ultrasound to determine the puncture depth: use B-ultrasound to determine the position of the gonad of the specimen and the thickness H of the muscle layer.

[0019] (3) 2 mm slit in the epidermis of the specimen: Use a sterilized scalpel to puncture a 2 mm wide slit parallel to the body axis of the fish at the target position. The tip of the finger is close to the tip of the scalpel, and the belly of the finger is in contact with the tip of the scalpel at the same time. Sturgeon skin, pierce the skin of the sturgeon with downward force, and the width of the opening gap is about 2mm;

[0020] (4) Determination of the puncture position: read the needle length position o...

Embodiment 2

[0024] Fat Sampling of Sturgeon Specimens

[0025] (1) Preparation before puncture: Disinfect the puncture needle, put it into the puncture gun, adjust it well, and make it in the triggering state. German automatic puncture gun MG15-22, Bard puncture needle MN2016, the sampling length is set to 22mm;

[0026] (2) B-ultrasound to determine the puncture depth: use B-ultrasound to determine the position of the gonad of the specimen and the thickness H of the muscle layer;

[0027] (3) 3 mm slit in the epidermis of the specimen: Use a sterilized scalpel to puncture a 3 mm wide slit parallel to the fish length body axis at the target position, with the tip of the finger close to the tip of the scalpel, and the belly of the finger and the tip of the scalpel at the same time. Touch the skin of the sturgeon, force it downwards, pierce the skin of the sturgeon, and the width of the opening gap is about 3mm;

[0028] (4) Determination of the puncture position: read the needle length p...

Embodiment 3

[0032] Sampling of the testes of sturgeon specimens

[0033] (1) Preparation before puncture: Disinfect the puncture needle, put it into the puncture gun, adjust it, and make it in the trigger state. German automatic puncture gun, MG15-22, Bard puncture needle MN1820, the sampling length is set to 15mm;

[0034] (2) B-ultrasound to determine the puncture depth: use B-ultrasound to determine the position of the gonad of the specimen and the thickness H of the muscle layer;

[0035] (3) 2 mm slit in the epidermis of the specimen: Use a sterilized scalpel to pierce a 2 mm wide slit parallel to the fish length body axis at the target position, with the tip of the finger close to the tip of the scalpel, and the belly of the finger and the tip of the scalpel at the same time. Touch the skin of the sturgeon, force it downwards, pierce the skin of the sturgeon, and the width of the opening gap is about 2mm;

[0036] (4) Determination of the puncture position: Read the needle length ...

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Abstract

A full-automatic sturgeon gonad tissue puncture sampling method comprises the following steps of preparation before puncture, puncture depth determination through B-mode ultrasound, slitting in an epidermal layer of a specimen for 2-3 mm, puncture position determination, sampling trigger and full-automatic emission gun storage. By adopting the full-automatic sturgeon gonad tissue puncture samplingmethod, sturgeon gonad tissue sampling can be achieved, and the sampling success rate can be also greatly improved.

Description

technical field [0001] The invention belongs to the field of histological research, in particular to a fully automatic puncture sampling method for gonad tissue of sturgeon. Background technique [0002] Worldwide, sturgeons are large in size, high in scientific research value and economic value, and have special habitat requirements. Due to the overfishing of humans, the isolation of water conservancy projects, the destruction of their habitats by water conservancy projects, and the impact of environmental pollution, the number of sturgeon populations has declined sharply, and sturgeon has become an endangered or even critically endangered protected species. In my country, represented by the Chinese sturgeon, there are 8 species of sturgeon distributed, and the hybrid sturgeon, which is an economic fish, faces many bottlenecks in its protection and research. For example, no satisfactory method has been found for the determination of the sex of sturgeon in the second phase,...

Claims

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Application Information

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IPC IPC(8): A61B10/02
Inventor 李伟管波廖小林朱滨田华常剑波
Owner WATER ENG ECOLOGICAL INST CHINESE ACAD OF SCI
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