An in vitro animal trachea model establishment method and evaluation method for evaluating tobacco mainstream smoke-induced inflammatory response
A mainstream smoke and evaluation method technology, applied in biochemical equipment and methods, biological testing, microbial determination/inspection, etc. System stability, high accuracy, good repeatability, and wide application range
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0049] The establishment method of the animal in vitro trachea model used for evaluating tobacco mainstream smoke-induced inflammation of the present invention (flow process is as follows: figure 1 shown), including the following steps:
[0050] (1) Trachea isolation: Sprague-Dawley (SD) male rats (SPF grade) were provided by Beijing Experimental Animal Platform (SCXK). Rats were deeply anesthetized with isoflurane on an anesthesia machine before performing surgical manipulations on the animals. Adult male SD rats of SPF grade purchased from Beijing Weitong Lihua Center Company, weighing 230±20g. All experimental animals were given sufficient water and food to allow them to drink and eat freely. The room temperature was maintained at 23±2°C, and the room was regularly disinfected.
[0051] ① When the toe of the rat is still unresponsive with surgical forceps, it means that the rat has entered a state of deep anesthesia. At this time, the rat's neck is dissected immediately. ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com