Halomonas taeanensis and method for preparing flocculants by same

A technology of Halomonas and a flocculant, which is applied in the field of marine Halomonas and the preparation of flocculants by using the same, can solve the problems of low flocculation activity and the like, and achieve the effects of strong flocculation effect, promoting sedimentation and strong flocculation ability.

Active Publication Date: 2018-01-30
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem of low flocculation activity in the preparation of extracellular polysaccharide flocculants by microorganisms at the present stage, the purpose of the present invention is to provide a strain of marine Halomonas that can secrete extracellular polysaccharides with flocculation, and at the same time provide The method for preparing the flocculant by the strain, the flocculant prepared from the exopolysaccharide secreted by the Halomonas marine strain has high flocculation activity, strong flocculation ability and stable performance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for preparing flocculant with Halomonas marine bacteria strain GHF11, comprising the following steps:

[0030] (1) Inoculate the strain on the solid medium and culture it at 25°C for 36 hours, then add sterile water to make the strain liquid, the volume ratio of sterile water to solid medium is 1:1;

[0031] (2) Inoculate the seed liquid into the liquid medium, the inoculation concentration is 0.6mL of the seed liquid / 100mL liquid medium, ferment and cultivate at 30°C for 3 days, and the shaker speed is 80r / min, to prepare the fermentation liquid;

[0032] (3) Add flocculation carrier to the fermented bacterial liquid, the addition amount is 5g / 100mL fermented bacterial liquid, the flocculated carrier is sandy soil, preferably is the natural sandy soil of cultivating Philippine clams, and the flocculated carrier is sterilized after being sieved through a 200-mesh sieve. , continue to ferment and cultivate for 1 day to obtain raw material liquid;

[0033] (4) A...

Embodiment 2

[0036] A method for preparing flocculant with Halomonas marine bacteria strain GHF11, comprising the following steps:

[0037] (1) Inoculate the strain on a solid medium and culture it at 28°C for 42 hours, then add sterile water to make a strain solution, the volume ratio of sterile water to solid medium is 1.25:1;

[0038] (2) Inoculate the seed liquid into the liquid medium, the inoculation concentration is 0.8mL of the seed liquid / 100mL of the liquid medium, ferment and cultivate at 28°C for 4 days, and the shaker speed is 120r / min, to prepare the fermented bacterial liquid;

[0039] (3) Add the flocculation carrier to the fermented bacterial liquid, the addition amount is 7.5g / 100mL fermented bacterial liquid, the flocculated carrier is sandy soil, preferably the natural sandy soil of cultivating Philippine clams, and the flocculated carrier is sterilized after being sieved through a 250-mesh sieve use, continue to ferment and cultivate for 2 days to obtain raw material l...

Embodiment 3

[0043] A method for preparing flocculant with Halomonas marine bacteria strain GHF11, comprising the following steps:

[0044] (1) Inoculate the strain on a solid medium and culture it at 30°C for 48 hours, then add sterile water to make a strain liquid, the volume ratio of sterile water to solid medium is 1.5:1;

[0045] (2) Inoculate the seed liquid into the liquid culture medium at an inoculation concentration of 1.0 mL of the seed liquid / 100 mL of the liquid medium, ferment and cultivate it at 30° C. for 5 days, and the shaker speed is 150 r / min to prepare a fermented bacterial liquid;

[0046] (3) Add the flocculation carrier to the fermented bacterial liquid, the addition amount is 10g / 100mL fermented bacterial liquid, the flocculated carrier is sandy soil, preferably the natural sandy soil for cultivating Philippine clams, and the flocculated carrier is sterilized after being sieved through a 200-mesh sieve. , continue to ferment and cultivate for 3 days to obtain raw m...

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PUM

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Abstract

The invention relates to the technical field of water treatment, in particular to Halomonas taeanensis and a method for preparing flocculants by the same. Bacterial strain Halomonas sp.GHF11 of the Halomonas taeanensis is separated and purified from sludge spitted by short-necked clams, cultures of the bacterial strain are subjected to amplification culture to obtain culture liquid, the culture liquid is subjected to fermenting culture to secrete exopolysaccharides, flocculation carriers are added into fermentation bacteria solutions in later period of fermentation to provide adhesion places for thalli, the thalli are adhered to cluster and settle, and the exopolysaccharides completely settle under the further action of ethyl alcohol. The exopolysaccharides secreted by the bacterial strainHalomonas sp.GHF11 has high flocculation effect; the carriers are added during preparation of the flocculants, exopolysaccharide sedimentation is accelerated, and consumption of the ethyl alcohol isreduced; the obtained flocculants have high flocculation capability and stable performance.

Description

technical field [0001] The invention relates to the technical field of water treatment, in particular to a strain of Halomonas marinum and a method for preparing flocculant using it. Background technique [0002] my country's urbanization has developed rapidly in the past ten years, but due to the lag in investment in sewage treatment facilities, low sewage treatment efficiency, and the discharge of a large amount of industrial wastewater and domestic sewage, the serious water environment has deteriorated, and the content of heavy metals, pigments, and suspended particles in the water body exceeds the standard. The outbreak of algae such as blue-green algae, water resources have now become an important factor restricting the development of social urbanization and industrialization. Improving sewage treatment technology and strengthening urban sewage treatment capacity has become a top priority. Among them, introducing flocculent particles of appropriate particle size into th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C02F1/56C12R1/01
Inventor 穆军崔霞杨桥
Owner ZHEJIANG OCEAN UNIV
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