Membrane type monolayer cell sheet manufacturing machine and sheet manufacturing method thereof
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A single-layer cell and tableting machine technology, applied in the field of pathological diagnostic equipment, can solve the problems of small throughput of membrane-type preparation samples, no cells in the center of the sampling area, unstable preparation effect, etc., which is beneficial to DNA staining and equipment. Small, lightweight effect
Pending Publication Date: 2018-01-30
MOTIC XIAMEN MEDICAL DIAGNOSTICS SYST
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Problems solved by technology
[0004] The technical problem to be solved by the present invention is to provide a membrane-type single-layer cell slicer and its slice-making method to solve the problem of l
Method used
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Embodiment 1
[0060] Place the sample bottle containing the cell sample on the shaker, shake and mix, unscrew the cap, put 1 cervical sample bottle into the sample bottle slot of the fixed plate (specification 1), and put the labeled One glass slide and one filter cartridge are respectively put into the slide slot and the film cartridge slot of the same fixed disk.
[0061] Insert the fixed disk on the bearing platform, select program 1, the number of specimens 1, and click run.
[0062] Reset the mechanical arm, turn it to the top of the filter cartridge slot, then move the robot arm down, pull the filter cartridge, and move up; turn it to the sample bottle slot again, and move the robotic arm down until the filter cartridge is immersed in the sample. After blowing the sample, The cells are sucked and filtered onto the filter membrane, the robotic arm moves up, the membrane cartridge loading device rotates 180°, and the remaining liquid is aspirated and reset; it is rotated again to the top of ...
Embodiment 2
[0064] Place the sample bottle containing the cell sample on the shaker, shake and mix, unscrew the cap, put a cervical sample bottle into the sample bottle slot of the fixed plate (specification 2), and put the labeled Put 2 slides and 1 filter cartridge into the slide slot and the membrane cartridge slot of the same fixed disk;
[0065] Embed the fixed disk on the bearing platform, select program 2, number of specimens 1, and click run;
[0066] Reset the mechanical arm, turn it to the top of the filter cartridge slot, then move the robot arm down, pull the filter cartridge, and move up; turn it to the sample bottle slot again, and move the robotic arm down until the filter cartridge is immersed in the sample. After blowing the sample, The cells are sucked and filtered onto the filter membrane, the robotic arm moves up, the membrane cartridge loading device rotates 180°, and the remaining liquid is aspirated and reset; it is rotated again to the top of the slide card slot, and th...
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Abstract
The invention provides a membrane type monolayer cell sheet manufacturing machine. The membrane type monolayer cell sheet manufacturing machine comprises a carrying platform, a mechanical arm, a pneumatic waste liquid collection system and a control system, wherein a fixed disc is embedded into the upper part of the carrying platform and is provided with a working clamp groove; the mechanical armis fixedly arranged at the dead center of the upper part of the carrying platform, and can do circumference and lifting movement along a center shaft; the mechanical arm comprises a membrane cylinderloading device, a vertical support part and a liquid drain pipe; the membrane cylinder loading device is used for automatically loading a membrane cylinder, draining liquid, transferring a sample, andunloading the membrane cylinder; one end of the liquid drain pipe is connected to a liquid drain circular hole in the membrane cylinder loading device, and the other end of the liquid drain pipe is connected with a waste liquid collecting bottle. The sheet manufacturing method comprises the following steps of sucking cells onto a membrane by negative pressure, enabling the mechanical arm to transfer the cells onto a slide, treating the cell sheet by a special immobilizing solution, and performing pathological staining. The membrane type monolayer cell sheet manufacturing machine has the advantages that the labor operation amount is reduced, the sample flux is large, the manufacturing is standard, the cells are uniformly distributed, the stability and reliability are realized, and the large-scale popularization and application effects are realized.
Description
Technical field [0001] The invention relates to the field of pathological diagnosis instruments, in particular to a membrane-type monolayer cell sheeting machine and a sheeting method thereof. Background technique [0002] As early as the 1960s and 1970s, many medical units in Europe and the United States used filter membranes to manually produce films. The quality of the films was greatly affected by the experience of the operators, and the film production effects were not ideal and the efficiency was not high. Until 1997, the Thin Prep thin layer production technology (TCT) has overcome the defects of traditional production methods in cell overlap, blurred background, and poor cell fixation. It has significantly improved the uniformity of cell distribution, clear production background, and diagnosis. The accuracy has been greatly improved, and liquid-based thin-layer filming technology has become popular all over the world. [0003] The liquid-based thin-layer film preparation t...
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Application Information
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