A dual-signal bioelectrochemical method for detecting water toxicity

A bioelectrochemical, dual-signal technology, applied in the field of biochemical detection, can solve problems such as time extension and achieve low-cost results

Active Publication Date: 2020-06-26
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, since the electrical activity of Shewanella is inhibited by water toxicity, the time required for the output current to reach the maximum value in the three-electrode system will also be prolonged with the increase of toxicity

Method used

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  • A dual-signal bioelectrochemical method for detecting water toxicity
  • A dual-signal bioelectrochemical method for detecting water toxicity
  • A dual-signal bioelectrochemical method for detecting water toxicity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Strain used: Shewanella oneidensis, purchased from ATCC American Type Culture Collection, strain number ATCC700550.

[0042] (2) Obtaining Shewanella bacterium liquid: insert Shewanella bacterium into LB medium (containing yeast extract 5g / L, tryptone 10g / L and sodium chloride 5g / L, pH=7.0) Species were cultivated overnight at 30°C with a shaking rotation speed of 200rpm to obtain bacterial liquid.

[0043] (3) Preparation of reaction buffer for bioelectrochemical sensors: 0.6ml LB medium and 11.4ml M9 medium were thoroughly mixed, and sodium lactate, calcium chloride, and magnesium sulfate were added to make the final concentrations respectively 18mM, 0.1mM, and 1mM.

[0044] (4) Centrifuge the overnight cultured Shewanella liquid at 5000rpm, and add the obtained bacterium pellet into the reaction buffer solution, and control the OD value at 1.0.

[0045] (5) Assembly of the bioelectrochemical sensor: the working electrode, saturated calomel electrode, and platinum ...

Embodiment 2

[0050] It is basically the same as Example 1, but with the following changes: the OD value of the bacteria is controlled at 0.5. Current suppression results as image 3 shown. A bacterial cell OD value of 0.5 can also detect the toxicity of DCP.

Embodiment 3

[0052] It is basically the same as Example 1, but with the following changes: the OD value of the bacteria is controlled at 1.5. Current suppression results as image 3 shown. The cell OD value of 1.5 can also detect the toxicity of DCP.

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Abstract

The invention relates to a double-signal bioelectrochemical method for detecting water toxicity and belongs to the technical field of biochemical detection. Voltage control and current signal recording are realized by using a simple electrochemical instrument, so that detection cost is low; electrochemical activity of shewanella is taken as a toxicity index, concentration range of water toxicity,suppression rate of maximum output current and time needed for cells to reach maximum current have a good linear relation, and double-signal detection of water toxicity can be realized.

Description

technical field [0001] The invention relates to a dual-signal bioelectrochemical method for detecting water body toxicity, belonging to the technical field of biochemical detection. Background technique [0002] Over the past century, the rapid development of industry and agriculture has made the environment more and more seriously polluted. Especially in the water environment, a large amount of organic matter, heavy metals, and pesticides enter the water environment and become the main source of water pollution. In order to strengthen the safety of river water and drinking water, my country has included water quality toxicity in the control standards in recent years. [0003] The commonly used water quality toxicity detection methods are mainly physical and chemical methods and biological methods. Physical and chemical methods use complex physical and chemical instruments to detect toxic pollutants in water, such as halogenated hydrocarbons, herbicides, pesticides, heavy ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30G01N27/48
Inventor 雍阳春杨园
Owner JIANGSU UNIV
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