Application of icariside II in preparing anti-hepatitis-B-virus products

A technology of hepatitis B virus and icariin, which is applied in antiviral agents, medical preparations containing active ingredients, antitumor drugs, etc.

Active Publication Date: 2018-02-09
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Icariside Ⅱ (icarisid Ⅱ, Ics Ⅱ) is a polyhydroxy flavonoid monomer component extracted from Epimedium, and is one of the active ingredients of Epimedium. It has been reported in the literature that it can treat erectile dysfunction, resist Ischemic brain damage, anti-tumor effects, etc., there is no report on the anti-hepatitis B virus effect of icariside Ⅱ

Method used

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  • Application of icariside II in preparing anti-hepatitis-B-virus products
  • Application of icariside II in preparing anti-hepatitis-B-virus products
  • Application of icariside II in preparing anti-hepatitis-B-virus products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: In vitro cell model experiment of icariside II

[0023] The present invention uses the method of CCK8 analysis to detect the influence of medicine on cell growth, such as figure 1 As shown, HepG2.2.15, an HBV-infected cell model recognized by experiments in the field, was used, and the concentrations of icariside Ⅱ were 5 μg / ml, 10 μg / ml, 12.5 μg / ml, 15 μg / ml and 20 μg / ml, respectively. The data were analyzed by T test, and the results showed that icariside Ⅱ with a concentration greater than 15 μg / ml had a significant effect on the growth of HepG2.2.15 cells (p<0.05).

[0024] Hepatitis B virus surface antigen (HBsAg) is the main indicator of HBV infection. Such as figure 2 As shown, by detecting and analyzing the amount of HBsAg in the cell supernatant, the results showed that when the concentration of icariside II was greater than 6.25 μg / ml, the secretion of HBsAg could be significantly inhibited (p<0.01).

[0025] Hepatitis B virus e antigen (HBeAg) ...

Embodiment 2

[0028] Example 2: HBV-infected mouse in vivo model experiment of icariside Ⅱ

[0029]The mature HBV plasmid pAAV HBV 1.2 in the field was used to establish the mouse model of HBV infection through tail vein injection, and the method of intraperitoneal injection was used to collect blood and separate serum for detection after 10 days and 20 days of administration.

[0030] After the body is infected with HBV, the first manifestation of HBsAg in the peripheral blood is increased. By detecting and analyzing the amount of HBsAg in mouse serum, such as Figure 5 Shown: after 10 days of administration, icariside II can significantly inhibit the secretion of HBsAg (p<0.05), and after 20 days of administration, the inhibitory effect is further enhanced (p<0.05).

[0031] HBeAg is a soluble protein that can be dissociated in serum, and its growth and decline are basically consistent with those of virions and DNA polymerase, and are indicators of HBV replication. By detecting and anal...

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Abstract

The invention discloses application of icariside II in preparing anti-hepatitis-B-virus products. Research results of in-vitro hepatitis B virus infected cell model experiments show that icariside IIcan inhibit expression of hepatitis B virus surface antigen and e antigen and can inhibit copying of hepatitis B virus DNA; in-vivo researches through a HBV infected mouse model show that icariside IIcan lower HBsAg, HBeAg and HBVDNA levels in HBV infected mouse serum. It is proved that icariside II has obvious anti-hepatitis-B-virus effect and can be used for preparing drug resistant to relateddiseases caused by hepatitis B virus infection.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to the application of icariside II, a specific monomer in the Chinese herbal medicine Epimedium, in the preparation of pharmaceutical compositions, especially the application of icariside II in the preparation of anti-hepatitis B virus products use. Background technique [0002] Hepatitis B virus (HBV)-related diseases have become one of the major diseases threatening human health today. At present, antiviral drugs for hepatitis B mainly include nucleoside analogs and interferon. Although these drugs have certain curative effects, they also have adverse reactions, and drug resistance against nucleoside analogs is gradually increasing. Therefore, the research of new anti-HBV drugs has more important significance. [0003] The traditional Chinese medicine Epimedium brevicornu Maxim, also known as Xianlingpi, Qianliangjin, Fangzhangcao, etc., is a plant of the genu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P31/20A61P1/16A61P35/00
CPCA61K31/7048
Inventor 王欢肖冬焱刘正芸罗果龚其海
Owner ZUNYI MEDICAL UNIVERSITY
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