Alkaloid compounds extracted from green bamboo standard and extraction method

A technology of alkaloids and extraction methods, which is applied in the direction of organic chemical methods, drug combinations, sugar derivatives, etc., can solve problems such as research, properties and uses of new alkaloid compounds, and achieve simple preparation processes and good pharmaceuticals. Wide range of effects with foreground, sources

Active Publication Date: 2020-05-05
SHANDONG ANALYSIS & TEST CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Regarding the separation and purification of new alkaloid compounds and extraction methods from the green bamboo standard, there is no research report, let alone the research on the properties and uses of the new alkaloid compounds in the green bamboo standard

Method used

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  • Alkaloid compounds extracted from green bamboo standard and extraction method
  • Alkaloid compounds extracted from green bamboo standard and extraction method
  • Alkaloid compounds extracted from green bamboo standard and extraction method

Examples

Experimental program
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Embodiment 1

[0118] The preparation method of compound I-VI, the steps are as follows:

[0119] (1) Take 5kg of green bamboo standard medicinal material, pulverize, extract under reflux with 95% ethanol, the solid-to-liquid ratio is 1:3, extract three times, the time is 2h, 1h, 1h respectively, combine the filtrate, vacuum rotary steaming, freeze-drying, to obtain Green bamboo standard crude extract 1kg;

[0120] (2) Add an appropriate amount of water to the total crude extract and ultrasonically disperse it, extract it with petroleum ether, ethyl acetate and n-butanol respectively, filter the extract, concentrate under reduced pressure to obtain petroleum ether, ethyl acetate, n-butanol and water extraction respectively part;

[0121] (3) Dissolve 180 g of the obtained ethyl acetate portion in methanol, add 300 g of 200-300 mesh silica gel to mix the sample, evaporate the solvent, and use the volume ratio CH 2 Cl 2 -MeOH (100:1→50:1→25:1→15:1→10:1→5:1→1:1→0:1) for gradient elution to o...

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Abstract

The invention discloses an alkaloid compound extracted from scindapsus officinalis schott, and an extraction method. The extraction method comprises the following steps: (1) dispersing a scindapsus officinalis schott crude extract into water, performing extracting with petroleum ether and ethyl acetate in sequence, and removing a solvent form the ethyl acetate organic phase after extraction to obtain an ethyl acetate extract; (2) dissolving the ethyl acetate extract into methanol, and performing gradient elution by a silica gel column chromatographic column, wherein the gradient eluant is CH2Cl2-MeOH, 5000 ml of an elution part is subjected to gradient elution and each 500 ml of the elution part is one receiving volume; (3) combining and concentrating the first five receiving columns of the gradient elution part with the volume ratio of 15:1 in the step (2), passing through C18 absorbing resin chromatography, and eluting with MeOH with different concentration sequentially; (4) enablingthe elution part of the MeOH with the specific concentration in the step (3) to pass through a C18 absorbing resin chromatographic column, and eluting with MeOH with different concentration in sequence; and (5) preparing the elution part of the MeOH with the specific concentration in the step (4) by a CH3CN-H2O mobile phase.

Description

technical field [0001] The invention relates to the technical field of alkaloid compounds and preparation methods thereof, in particular to alkaloid compounds extracted from green bamboo leaves and an extraction method. [0002] technical background [0003] Green Bamboo Standard (Scindapsus officinalis Schott), also known as Scindapus officinalis Schott, is a whole plant of Scindapus officinalis Schott, which is mainly distributed in Yunnan, Guizhou, and Guangxi. It is a rare ethnic group that is widely used locally. medicine. "Yunnan Chinese Herbal Medicine Selection" records that it has the effects of dispelling blood stasis, analgesia, moistening lung and relieving cough, and can cure bruises, fractures, rheumatic numbness, bronchitis, and pertussis; Bruises, rheumatism, carbuncle"; "Guizhou Medicine Plant Catalogue" records its "removing blood stasis to generate new, analgesic". There is no research report on the new alkaloid compounds isolated and purified from the gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D223/16C07D207/333C07H17/02C07C235/34C07C231/24A61P15/00A61P35/00
CPCA61K36/888C07B2200/07C07C235/34C07D207/333C07D223/16C07H17/02
Inventor 于金倩王晓闫慧娇耿岩玲王学勇赵恒强
Owner SHANDONG ANALYSIS & TEST CENT
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