Application of Passiflora Officinalis Extract in Preparation of Drugs for Prevention and Treatment of Breast Cancer

A breast cancer and extract technology, which is applied to the application field of passionflower extract in the preparation of breast cancer prevention and treatment drugs, can solve the problems of large adverse reactions and low water solubility, achieve good anti-breast cancer activity, inhibit proliferation, The effect of inhibiting the cell cycle of breast cancer

Active Publication Date: 2022-07-26
HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Discovering active drugs for treating tumors from plants has always been the research direction of global medical researchers. Paclitaxel from plants is widely used in the treatment of breast and ovarian cancer, but it has disadvantages such as low water solubility and large adverse reactions.

Method used

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  • Application of Passiflora Officinalis Extract in Preparation of Drugs for Prevention and Treatment of Breast Cancer
  • Application of Passiflora Officinalis Extract in Preparation of Drugs for Prevention and Treatment of Breast Cancer
  • Application of Passiflora Officinalis Extract in Preparation of Drugs for Prevention and Treatment of Breast Cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Extraction of active ingredients from Passiflora vine leaves

[0033] Experimental material: Purple Passiflora (Purple Passiflora P. edulis ) of the vine leaves were collected from Chengmai, Haikou City, Hainan Province.

[0034] The collected vine leaves were dried in an oven at 40°C, pulverized, passed through a 40-mesh sieve, soaked in 80% ethanol at a volume ratio of 1:5, and extracted by ultrasonic for 3 times, 30 min each time. The extract is concentrated into an extract extract through reduced pressure rotary evaporation, and the extract extract is freeze-dried to obtain an extract dry powder; the extract dry powder is dissolved in water to form an extract dry powder solution, and then an extractant petroleum ether is added for extraction. The liquid was evaporated under reduced pressure to obtain Passiflora vine leaf extract.

[0035] The volume of the extractant is twice that of the extract dry powder solution. The volume ratio of described ethano...

Embodiment 2

[0036] Example 2 CCK-8 method to measure the activity of inhibiting the proliferation of breast cancer cells

[0037] Dissolve the Passiflora vine leaf extract of Example 1 with DMSO solvent to prepare the Passiflora vine leaf extract solutions of different concentrations (31, 62.5, 125, 250, 500 μg / mL) for cell viability test, and make the culture medium. The DMSO content is less than 0.1%.

[0038] In a 96-well plate, about 4000 MDA-MB-231 cells were inoculated in each well, and after the cells adhered to the wall, the above DMEM medium (containing 0.25% HEPES) of the Passiflora vine leaf extract of different concentrations was added, and 0.1% DMSO was added. Medium is a negative control. Add CCK-8 after 72h of treatment, put in CO 2 After 1 h in the incubator, the absorbance at 405 nm was read with a microplate reader, and cell viability = (A blank group-A treatment group) / A blank group × 100%.

[0039] The experimental results show that the ethanol extracts of Passiflor...

Embodiment 3

[0041] Example 3 Scratch method to measure the activity of inhibiting migration of breast cancer cells

[0042] 24-well plate by 2×10 per well 5MDA-MB-231 cells in logarithmic growth phase were inoculated with the number of cells, and after 24 hours of adherence, the cells were scratched with a pipette tip, the floating cells were washed away with PBS, and the Passiflora vine leaf extract described in Example 1 was dissolved in DMSO. Passiflora vine leaf extracts of different concentrations (25, 50, 100, 200, and 400 μg / mL) were obtained. After 24 hours, the cells were stained with crystal violet and photographed under a microscope. The changes in the scratch area before and after treatment were counted, and the inhibitory migration rate was calculated. .

[0043] The experimental results show that: different concentrations of Passiflora vine leaf extracts have the activity of inhibiting the migration of MDA-MB-231 cells, and the average inhibition rate of 400 μg / mL treatment...

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Abstract

The invention discloses the application of a passionflower vine leaf extract in preparing a medicine for preventing and treating breast cancer. The active components in the passionflower vine leaf extract comprise isoorientin, vitexin and isovitexin flavonoids. The invention also discloses an extraction method of the passionflower vine leaf extract. The Passiflora vine leaf extract described in this application can inhibit the proliferation of breast cancer cells, inhibit the migration of breast cancer cells, and inhibit the cell cycle of breast cancer; it can be used for the preparation of drugs for the prevention and treatment of breast cancer, as well as for other Anti-cancer drugs.

Description

technical field [0001] The present application relates to the application field of plant extracts, in particular to the application of passionflower extracts in the preparation of medicines for preventing and treating breast cancer. Background technique [0002] Passionflower ( Passiflora edulis ), also known as "passion fruit", belongs to the Passiflora family of Passifloraceae in the order Malpighiales. Passiflora is native to the tropical and subtropical regions of South America and Brazil. It has been introduced and cultivated all over the world because of its beautiful flower color and vine leaves with both shading and medicinal functions. There are about 500 species of Passiflora in the world, and about 50 species are edible. There are more than 20 species of Passiflora in my country, mainly distributed in Guangdong, Guangxi, Fujian, Guizhou, Yunnan and Hainan. Passionflower ( P. edulis ) fruit can be used to make jam, juice and red wine, etc. It is an important e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P15/14A61P35/00A61P35/04A61K135/00A61K127/00
CPCA61K36/185A61P15/14A61P35/00A61P35/04A61K2236/333A61K2236/35A61K2236/51
Inventor 马伏宁宋顺黄东梅许奕吴斌邢文婷徐兵强郭刚王达新
Owner HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
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