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Ketamine-induced schizophrenia animal model and mechanism study
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A schizophrenia and animal model technology, applied in the field of ketamine-induced schizophrenia animal model and its mechanism research, can solve the problems of difficult treatment, unknown etiology of schizophrenia, lack of biological detection indicators, etc. Effect
Inactive Publication Date: 2018-03-16
KUNMING MEDICAL UNIVERSITY
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[0007] The etiology of schizophrenia is unknown, and there are few studies on its pathogenesis at present. The clinical diagnosis mainly depends on the judgment of positive symptoms and negative symptoms, and there is a lack of objective biological detection indicators. Its treatment has always been a difficult problem in the medical field. Therefore, it is particularly important to establish experimental animal models of schizophrenia.
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Embodiment 1
[0027] 1. Experimental animals
[0028] Take 40 Kunming mice, male, weighing 25-30 grams, clean grade. Before the experiment, the mice were raised in the animal room for at least 3 days, maintained at room temperature (22±1)°C, and the humidity was 50-60%. During the whole experiment, the mice were free to drink and eat.
[0029] 2. Establishment and grouping of animal models of schizophrenia
[0030] 40 mice were randomly divided into 4 groups, normal saline control group, ketamine low dose (25 mg / kg), medium dose (50 mg / kg) and high dose (100 mg / kg) group. The control group and different doses of ketamine groups were injected with normal saline and ketamine once a day, respectively, for 7 consecutive days.
[0033] After 10 minutes of administration, the mice were placed in a 40cm×40cm×30cm homemade clean wooden field box, the surrounding wall was gray, and the bottom surface was divided into 25 square...
[0046] After administration, the mice in each group were collected blood by orbital bleeding method, and the brain tissue was taken out after the mice were sacrificed, and made into homogenate under freezing conditions, and the blood and homogenate were cryopreserved for biochemical determination.
[0047] 2. Determination of SOD content in serum and brain tissue
[0048] 3. Determination of MDA content in serum and brain tissue
[0049] 4. Immunohistochemical technique
[0050] The steps of immunohistochemical staining were mainly carried out according to the kit instructions of the reagent company, and high temperature and high pressure treatment was performed on the sections instead of trypsindigestion to promote antigenexposure.
[0051] 5. Statistics and analysis
[0052] Determination of biochemical indicators
[0053] Effects of Continuous Administration of Ketamine on Serum SOD and MDA Contents in Mice
[0054] Serum SOD activity ...
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Abstract
The present invention provides a ketamine-induced schizophreniaanimal model and mechanism study. The study includes the following steps: 1) ketamine-induced schizophreniaanimal model establishment;2) behavioral and morphological observation after ketamine-induced schizophrenia; 3) correlation with oxidative stress after ketamine-induced schizophrenia. Systemic observations are carried out on schizophrenia-like symptoms caused by single- or continuous-dose administration of small, medium, and large doses of ketamine to mice, the feasibility of establishing models of different doses and different administration ways is subjected to comparison and preliminary evaluation, and results show that single- or continuous-dose administration of ketamine can induce symptoms similar to schizophreniain mice and lead to a dose-dependent trend to a certain extent. In addition, through analysis, oxidative stress is correlative after ketamine-induced symptoms similar to schizophrenia of mice are developed, and a basis for study on subsequent ketamine addiction mechanism and schizophrenia is provided.
Description
technical field [0001] The invention relates to the technical field of animal models, in particular to a ketamine-induced schizophrenia animal model and the research on its mechanism. Background technique [0002] Ketamine (Ketamine, K) is a phencyclidine (phencylidine, PCP) derivative and a non-competitive N-methyl-D-aspartate (N-Methyl-D-Aspartate, NMDA) receptorantagonist. Ketamine has been used clinically as an intravenous anesthetic since 1970. Ketamine has two optical isomers, left-handed and right-handed, of which the right-handed isomer has a higher therapeutic index and fewer adverse reactions. Ketamine currently used clinically is a racemate of two enantiomers of dex-ketamine and L-ketamine. [0003] Ketamine is the only NMDA receptorantagonist approved by the U.S. Food and Drug Administration (FDA), and its mechanism of action is complex, involving NMDA receptors, opioid receptors, monoamine receptors, and acetylcholine receptors. and voltage-gated calcium ch...
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