Water chestnut plant antimicrobial extract and preparation method and application thereof

An antimicrobial and extract technology, applied in the field of medicine, can solve the problems of antimicrobial effect of water chestnuts that have not been reported, and achieve the effect of simple and practical extraction method, good inhibitory effect and good inhibitory activity

Inactive Publication Date: 2018-03-16
HEZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, so far, no research on the antimicrobial effects of water chestnuts has been reported.

Method used

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  • Water chestnut plant antimicrobial extract and preparation method and application thereof
  • Water chestnut plant antimicrobial extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A preparation method of the water chestnut plant antimicrobial extract of the present invention includes the following steps:

[0024] (1) The dried water chestnut plants (rhizome and branches and leaves) are crushed, placed in a subcritical extraction kettle, and 95% by mass methanol solution is added as the extraction solvent. Among them, the amount of methanol solution and water chestnut plants The ratio is 10mL:1g, the subcritical extraction is carried out for 10min under the pressure of 25MPa, temperature of 150℃, stirring speed of 200rpm and nitrogen protection;

[0025] (2) Centrifuge the slag liquid obtained in step (1) at room temperature and a rotation speed of 4000×g for 15 minutes, and collect the supernatant;

[0026] (3) Put the supernatant obtained in step (2) in the reactor of the molecular distillation apparatus, and proceed at a temperature of 80°C, a distillation pressure of 250 Pa, a feed rate of 2.0 mL / min, and a rotation speed of 250 r / min. Molecular dis...

Embodiment 2

[0028] A preparation method of the water chestnut plant antimicrobial extract of the present invention includes the following steps:

[0029] (1) Crush the dried water chestnut plants (rhizome and branches and leaves), place them in a subcritical extraction kettle, and add 75% by mass methanol solution as the extraction solvent. Among them, the amount of methanol solution and water chestnut plants The ratio is 11mL:1g, the subcritical extraction is performed for 8min under the pressure of 40MPa, temperature of 130°C, stirring speed of 200rpm and nitrogen protection;

[0030] (2) Centrifuge the slag liquid obtained in step (1) at room temperature and a rotation speed of 5000×g for 12 minutes, and collect the supernatant;

[0031] (3) Put the supernatant obtained in step (2) into the reactor of the molecular distillation apparatus, and proceed at a temperature of 85°C, a distillation pressure of 220 Pa, a feed rate of 2.5 mL / min, and a rotation speed of 300 r / min. Molecular distillati...

Embodiment 3

[0033] A preparation method of the water chestnut plant antimicrobial extract of the present invention includes the following steps:

[0034] (1) Crush the dried water chestnut plants (rhizome and branches and leaves), place them in a subcritical extraction kettle, and add 55% by mass methanol solution as the extraction solvent. Among them, the amount of methanol solution and water chestnut plants The ratio is 12mL:1g, the subcritical extraction is carried out for 8min under the pressure of 50MPa, temperature of 120℃, stirring speed of 150rpm and nitrogen protection;

[0035] (2) Centrifuge the slag liquid obtained in step (1) at room temperature and a rotation speed of 6000×g for 10 minutes, and collect the supernatant;

[0036] (3) Put the supernatant obtained in step (2) into the reactor of the molecular distillation apparatus, and proceed at a temperature of 90°C, a distillation pressure of 200 Pa, a feed rate of 3.0 mL / min, and a rotation speed of 350 r / min. Molecular distillat...

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PUM

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Abstract

The invention discloses a water chestnut plant antimicrobial extract and a preparation method and application thereof. The preparation method comprises the following steps: (1) crushing a dried waterchestnut plant, placing the crushed water chestnut plant in a subcritical extraction kettle, and performing subcritical extraction by taking a methanol solution as an extraction solvent; (2) centrifuging a residue liquid obtained in step (1), and collecting the supernate; and (3) placing the supernate obtained in step (2) in a reaction kettle of a molecular distillation instrument, performing molecular distillation, and collecting distillation cut to obtain the water chestnut plant antimicrobial extract. The preparation method is simple in preparation process and high in extraction ratio, theobtained water chestnut plant antimicrobial extract has good antimicrobial activity, and a certain foundation is laid for application of water chestnut to antimicrobial drug studies.

Description

Technical field [0001] The invention relates to the technical field of medicine, in particular to a water chestnut plant antimicrobial extract and a preparation method and application thereof. Background technique [0002] Eleochairis toberosa (Eleochairis toberosa), also known as water chestnut, is the underground bulb of a perennial aquatic herbaceous water chestnut in the Cyperaceae family. It is cultivated in all provinces south of the Yangtze River in my country. Horseshoe is not only juicy, sweet and nutritious, but also has good medicinal value. It has the reputation of "Underground Sydney" and "Jiangnan Ginseng" since ancient times. Traditional medicine of the motherland believes that horseshoe cold is sweet and has the effects of clearing heat, resolving phlegm, and eliminating accumulation. It can treat jaundice, hot showers, swelling, red eyes, sore throat and other diseases. It is a good medicine in medicine. The Dictionary of Traditional Chinese Medicine records tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/89A61P31/00
CPCA61K36/89A61K2236/333A61K2236/37A61K2236/51A61K2236/53
Inventor 杨金凤段振华
Owner HEZHOU UNIV
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