Producing method of antimicrobial peptide with enhanced adhesion and uses thereof

An antimicrobial peptide and antimicrobial technology, applied in botany equipment and methods, chemicals for biological control, medical preparations of non-effective ingredients, etc., can solve the problem of chemical toxicity, silver and copper do not have antimicrobial properties Activity, complex immobilization methods and other issues, to achieve the effect of no cytotoxicity and excellent antimicrobial activity

Inactive Publication Date: 2019-07-30
INTELLIGENT SYNTHETIC BIOLOGY CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a problem that silver and copper do not have sufficient antimicrobial activity and cause cytotoxicity due to their nanostructure
[0007] However, the conventional antimicrobial coating technology has problems in that because the method uses chemicals to treat the surface of the immobilized peptide through a multi-step reaction, its effect is discontinuous; or, the immobilization method is complicated and there is toxicity of the used chemicals question

Method used

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  • Producing method of antimicrobial peptide with enhanced adhesion and uses thereof
  • Producing method of antimicrobial peptide with enhanced adhesion and uses thereof
  • Producing method of antimicrobial peptide with enhanced adhesion and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1: Preparation of Adhesive Antimicrobial Peptides

Embodiment 1-1

[0060] Example 1-1: Adhesiveness imparted to antimicrobial peptides

[0061] In order to utilize NKC, a peptide with strong antibacterial activity against various microorganisms, as an antimicrobial coating agent, the antimicrobial peptide was endowed with adhesion by conjugating 3,4-dihydroxyphenylalanine (DOPA) to the end of NKC properties, the aforementioned DOPA is known as an unnatural amino acid and plays an important role in providing the adhesion strength of mussel adhesion protein, which is biocompatible and exhibits strong adhesion regardless of the surface sex. In this paper, to determine the optimal number of DOPA, 1, 3, 5 or 7 DOPA moieties were conjugated to the end of NKC, and then GGGGS was added as a flexible linker sequence between NKC and DOPA sequences to prevent NKC and DOPA affect each other's activities. Amino acid sequence information for conjugated NKC and DOPA is shown in Table 1 below.

[0062] Table 1

[0063]

[0064] *DOPA is abbreviated as...

Embodiment 1-2

[0065] Examples 1-2: Identification of antimicrobial activity of adhesive antimicrobial peptides

[0066] In order to confirm the antimicrobial activity of the adhesive antimicrobial peptide prepared in Experimental Example 1-1, the minimum inhibitory concentration for inhibiting the growth of Escherichia coli was identified by using the method disclosed in Nature Protocols.2008; 3(2):163-175 (MIC). Briefly, media in which individual colonies were grown for 16 h was subcultured and grown until OD 600 0.4 to 0.6 is reached, which corresponds to logarithmic growth. Thereafter, calculate the cell number based on the OD value, and set the cell number as 10 6 cells / mL. When loading M-H Broth (MHB) into the polypropylene 96-well plate, add 90 µL to the first row of the plate and 100 µL to the control wells and 50 µL to the remaining wells. Thereafter, prepare a peptide solution with a concentration of 640 μM and add 10 μL of it to each well of the first row. Mix the peptide sol...

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PUM

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Abstract

The present invention relates to a method for preparing an antimicrobial peptide with enhanced adhesion, and an antimicrobial coating method of the antimicrobial peptide with enhanced adhesion. The antimicrobial coating method of the present invention can be widely used as a method for adding an antimicrobial property to medical products and industrial products, because the method is capable of coating the antimicrobial peptide with enhanced adhesion, which has no cytotoxicity and possesses an excellent antimicrobial activity, on various surfaces with optimal efficiency.

Description

technical field [0001] The present invention relates to a method for preparing an antimicrobial peptide with enhanced adhesion and an antimicrobial coating method for the antimicrobial peptide with enhanced adhesion. Background technique [0002] Recently, infectious diseases worldwide have been on the rise, with the result that more than $60 billion is spent annually on disease treatment. According to reports, 50,000 people die each year in the United States and Europe, and more than 1,000 people die each year in South Korea due to infectious diseases. Antibiotics are used to treat these infectious diseases, but the emergence of antibiotic-resistant superbugs due to overuse of antibiotics has led to an increase in infectious disease mortality (average annual increase of 15%; announced by the Korea Centers for Disease Control and Prevention). Furthermore, no new antibiotics have been developed since the 1980s. As infectious diseases become difficult to treat due to antibio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A01N37/46A01P3/00A01P1/00
CPCC07K7/08A01N37/46A61K38/10A61K38/1767A61K38/1787A61K47/64A61L26/0047A61L27/227A61L2202/21A61L2420/04
Inventor 金善昌黄英恩布乐明
Owner INTELLIGENT SYNTHETIC BIOLOGY CENT
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