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Synergist for promoting synthesis of brevibacillus laterosporus antimicrobial peptide and application of synergist

A technology of Bacillus brevius and synergist, applied in the biological field, can solve the problems of poor efficiency and low yield, and achieve the effects of simple use, strong metabolism and good economic benefits

Active Publication Date: 2018-03-16
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a synthetic synergist for antimicrobial peptides with high production efficiency and simple addition process for the problems of low yield and poor efficiency of the existing antimicrobial peptide fermentation

Method used

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  • Synergist for promoting synthesis of brevibacillus laterosporus antimicrobial peptide and application of synergist
  • Synergist for promoting synthesis of brevibacillus laterosporus antimicrobial peptide and application of synergist
  • Synergist for promoting synthesis of brevibacillus laterosporus antimicrobial peptide and application of synergist

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Experimental program
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Effect test

Embodiment 1

[0036] The antimicrobial peptide output under the different synergist concentrations of embodiment 1

[0037] Add different contents of synergists to the fermentation medium of Brevibacillus spp. AS 1.864 (the carbon and nitrogen sources are glucose and peptone, respectively), and culture in shake flasks at 30°C and 250r / min for 24h to measure the antibacterial activity of the antimicrobial peptides. The final concentration of each component of the synergist and the yield of antimicrobial peptides are shown in Table 1 below. Through the multiple comparison analysis of SPSS software, there was a significant difference between the CK control group and the 9 experimental groups added with synergists (P<0.05). It can be seen that the effect of adding synergists on the production of antimicrobial peptides was obvious.

[0038] Antimicrobial peptide production under different synergist component content in the medium of table 1

[0039]

[0040] Note: CK means no synergist added...

Embodiment 2

[0041]The impact of embodiment 2 synergistic application on antimicrobial peptide production

[0042] Add 10mmol / L glutamic acid, 80mmol / L threonine, 45mmol / L succinic acid to the fermentation medium of Brevibacillus lateralosporus AS 1.864 composed of glucose and peptone, and add 10mmol glutamic acid alone / L, threonine 80mmol / L, and succinic acid 45mmol / L were the control group, and those without synergists were the blank group. The above-mentioned culture medium was inoculated with the seeds of Brevibacillus sporogenes respectively, and cultured in shake flasks at 30° C. and 250 r / min for 24 hours. The production of antimicrobial peptides in the experimental group added with synergists was higher than that in the control group with glutamic acid, threonine and succinic acid alone, while the production of antimicrobial peptides in the control group with glutamic acid, threonine and succinic acid alone was lower higher than that of the blank group without synergist. The res...

Embodiment 3

[0045] Embodiment 3 synergists are used for the production of antimicrobial peptides in corn steep liquor fermentation medium

[0046] The synergist was added to the Brevibacillus disspora AS1.864 fermentation medium composed of glucose and corn steep liquor as carbon and nitrogen sources, wherein the final concentrations of glutamic acid, threonine and succinic acid in the medium were 10mmol / L, 80mmol / L and 45mmol / L, 30°C, 250r / min shake flask culture for 24h, the titer of the antimicrobial peptide was 2340AU / mL, and the bacterial cell OD 600 Value is 3.0, compared with no synergist group, antimicrobial peptide production has increased by 90.0%, thalline OD 600 The value increased by about 50.0%.

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Abstract

The invention provides a synergist for promoting synthesis of brevibacillus laterosporus antimicrobial peptide and an application of the synergist. The synergist consists of the following active ingredients: glutamic acid, threonine and succinic acid at the molar ratio of (10-50) to (20-170) to (10-60). The synergist provided by the invention, when used for fermenting brevibacillus laterosporus, can improve the yield of the antimicrobial peptide by 25-90% and the OD600 value of the bacterium (the brevibacillus laterosporus) by 40-80%. The synergist is easily available in raw materials, simplein using method and obvious in effect of promoting synthesis of the antimicrobial peptide; the synergist not only can improve the yield of the antimicrobial peptide, but also can promote the growth ofthe bacterium, improve production efficiency and shorten a fermentation cycle; and the synergist is good in economic benefits.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a synergist for promoting the synthesis of antibacterial peptides of Brevibacillus lateralsporosa and its application. Background technique [0002] Antimicrobial peptide is an abbreviation for a class of active polypeptides that can inhibit or kill bacteria and fungi. Due to the increasing abuse of antibiotics and the emergence of superbugs, it is urgent to find a new type of antibiotic substitute. question. Antimicrobial peptides have become an ideal substitute for antibiotics due to their stable physical and chemical properties, broad antibacterial spectrum, high-efficiency antibacterial effect, unique antibacterial mechanism, and safety and no residue. In recent years, they have attracted widespread attention and research. [0003] Brevibacillus laterosporus (Brevibacillus laterosporus) is a Gram-positive bacterium that widely exists in soil, seawater, etc., ...

Claims

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Application Information

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IPC IPC(8): C12P21/00C12R1/01
CPCC12P21/00
Inventor 王志新贾英民宁亚维李兴峰杨柯
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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