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A method for large-scale preparation of penicillic acid by marine Penicillium fungus

A technology of marine fungus and penicillic acid, applied in the field of preparing penicillic acid

Active Publication Date: 2019-02-22
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the preparation of penicillic acid by marine Penicillium fungi, let alone large-scale preparation of penicillic acid by marine Penicillium fungi

Method used

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  • A method for large-scale preparation of penicillic acid by marine Penicillium fungus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Cultivation of the marine fungus Penicillium sp.HK1-6

[0023] The medium used for the culture of the fungus Penicillium sp. (HK1-6) is to add in every 1000mL of water: 200g of potatoes boiled for juice, 20g of glucose, 30g of coarse sea salt, and 15g of agar; Make media plates. The fungal strains were inoculated on medium plates and cultured on a shaker at 20°C for 3 days.

[0024] (2) Fermentation of marine fungus Penicillium sp.HK1-6

[0025] The fermentation medium used for the fermentation of the fungus Penicillium sp.HK1-6 is as follows: add 200g of potatoes to boil for juice, 20g of glucose, and 30g of coarse sea salt for every 1000mL of water; when used, divide them into Erlenmeyer flasks. The fungal strain is inoculated in the culture medium of the Erlenmeyer flask, and cultured statically at 15-20° C. for 28 days.

[0026] (3) Separation and extraction of penicillic acid

[0027] Take 30L of the fermented product obtained in step (2), separate the ferm...

Embodiment 2

[0032] (1) Cultivation of the marine fungus Penicillium sp. (HK1-6)

[0033] The medium used for the culture of the fungus Penicillium sp. (HK1-6) contains 0.2% glucose (percentage by weight, the same below), 2% yeast extract, 0.02% peptone, 0.2% agar, and 5% coarse sea salt. The rest is water, which is made into a test tube slant when used, and the fungal strains are cultivated at 25°C for 7 days.

[0034] (2) Fermentation of marine fungus Penicillium sp. (HK1-6)

[0035] The fermentation medium used for the fermentation culture of the fungus Penicillium sp. (HK1-6) contains glucose 0.2% (percentage by weight, the same below), yeast extract 0.02%, peptone 2%, coarse sea salt 0.05%, an appropriate amount of water, The fungal strains were grown at 15°C for 35 days.

[0036] (3) Separation and extraction of penicillic acid

[0037] Get 50L of the fermented product obtained in step (2), separate the fermented liquid from the thalline, extract the fermented liquid 4 times with ...

Embodiment 3

[0041] (1) Cultivation of the marine fungus Penicillium sp.HK1-6

[0042] The medium used for the culture of the fungus Penicillium sp.HK1-6 contains 5% glucose (percentage by weight, the same below), 0.02% yeast extract, 2% peptone, 3% agar, 1% coarse sea salt, and the rest is Water, made into a test tube slant when used, and the fungal strains were cultured at 20°C for 4 days.

[0043] (2) Fermentation of marine fungus Penicillium sp.HK1-6

[0044] The fermentation medium used for the fermentation culture of the fungus Penicillium sp.HK1-6 contains 5% glucose (percentage by weight, the same below), 0.02% yeast extract, 2% peptone, 5% coarse sea salt, and the rest is water. Cultured at 20°C for 30 days.

[0045] (3) Separation and extraction of penicillic acid

[0046] Get 100L of fermented product of step (2) gained, after fermented liquid and thalline are separated, fermented liquid is extracted 2 times with dichloromethane, extract is concentrated under reduced pressure...

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Abstract

The invention belongs to the field of utilization of marine microbial resources, and in particular relates to a method for preparing penicillic acid by large-scale fermentation of marine Penicillium fungi. The method comprises the following steps: (1) carrying out strain culture to the marine fungus Penicillium sp.HK1-6 in the bacterial culture medium; (2) to the marine fungus Penicillium sp.HK1-6 in the fermentation medium again Penicillium sp.HK1‑6 is fermented and cultivated; (3) the fermentation broth obtained in step (2) is separated from the bacteria, the fermentation broth is extracted with an organic solvent, and the extracts are combined and then concentrated under reduced pressure to obtain a fermented broth extract The thalline is extracted with an organic solvent, and concentrated under reduced pressure after merging the extract to obtain the thalline extract; merging the fermentation liquid extract and the thalline extract, and carrying out chromatographic separation to obtain the crude product; (4) the crude product obtained in step (3) After recrystallization or chromatographic separation, the pure product of penicillic acid is obtained, the purity is over 98.0%, and the yield is as high as 500-650 mg / L.

Description

technical field [0001] The invention belongs to the field of utilization of marine microbial resources, and in particular relates to a method for preparing penicillic acid by large-scale fermentation of marine Penicillium fungi. Background technique [0002] Penicillic acid is a mycotoxin, which exists in two interconversion forms of linear substituted keto acid and lactone. Its molecular weight is 170.16, and it is easily soluble in hot water, ethanol, ether and chloroform. [0003] [0004] There are many reports about the pharmacological activity and toxicity of penicillic acid. It is reported that penicillic acid has antibacterial, antifungal, antiviral, antitumor activities, inhibits acetylcholinease, and inhibits NF-κB activation ("HETEROCYCLES", 2008, Volume 76, No. 2, pages 1561-1569) and other pharmacological activities. In addition, penicillin is toxic to humans and animals, mainly causing damage to organs such as the heart, liver and kidneys, and has potential ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P17/04C12R1/80
CPCC12P17/04C12N1/145C12R2001/80
Inventor 陈敏沈南星于跃
Owner YANGZHOU UNIV
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