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A strain of Enterobacter aerogenes degrading malachite green under high-salt environment

A technology of Enterobacter aerogenes and malachite green, which is applied in the field of environmental microorganisms to achieve high degradation efficiency and broad application prospects

Active Publication Date: 2020-02-07
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many studies have shown that the dyes of triphenylmethanes such as Pseudomonas, bacillus, and saccharomycetes have a decolorization function, and there are fewer reports on degrading malachite green for Enterobacter (Enterobacter) bacterial strains, and a strain that can be disclosed in CN104830729A Enterobacter sp. strain CV-v that degrades malachite green; so far, there is no report that Enterobacter aerogenes can degrade malachite green

Method used

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  • A strain of Enterobacter aerogenes degrading malachite green under high-salt environment
  • A strain of Enterobacter aerogenes degrading malachite green under high-salt environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Screening of Enterobacter aerogenes S27 and degradation of malachite green

[0022] Collect Suaeda salsa strains from coastal saline-alkali land in Dongying City, Shandong Province, wash them twice with sterile water, soak them in 75% alcohol for 3-5 minutes, wash them twice with sterile water, soak them in 0.1% mercury liter for 5 minutes, and soak them in sterile water for 5 minutes. Rinse 8 times. Take 100 μL of sterile water after the last rinse and smear the plate as a control. Take two sections of sterilized Suaeda salsa about 5cm into the mortar, add 2mL of sterile water, grind and take the supernatant, and dilute it in multiples. 100 μL of LB plates coated with 0.5%, 3% and 5% NaCl (w / v) concentrations were taken respectively, and cultured at 30° C. for 48 h. The isolated strain was purified by streaking method and dilution coating plate method, and purified Enterobacter aerogenes (Enterobacter aerogenes) S27 was obtained. The colony was viscous and ...

Embodiment 2

[0024] Example 2 Enterobacter aerogenes S27 salt tolerance test

[0025] The S27 strain was inoculated in LB medium and cultured overnight at 30°C in a shaker with a rotation speed of 150rpm to obtain an overnight culture; In 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14% LB liquid medium, observe the growth status of the strain ,As shown in Table 1. Strain S27 can tolerate the highest salt concentration of 13%, among which, it grows well at the salt concentration of 1-9%, grows moderately at the salt concentration of 10-11%, and grows slowly at the salt concentration of 12-13%. There was almost no growth at a salt concentration of more than 13%, indicating that the strain S27 had good salt tolerance.

[0026] Table 1 Growth status of Enterobacter aerogenes S27 under different NaCl concentrations

[0027] NaCl(w / v) Growth NaCl(w / v) Growth 1％ +++ 8％ +++ 2％ +++ 9％ +++ 3％ +++ 10％ ++ 4％ +++ 11％ ++ 5％ +++ 12％ + 6％ ...

Embodiment 3

[0029] Example 3 Degradation of Malachite Green by Enterobacter aerogenes S27 at different salt concentrations

[0030] Inoculate the S27 strain in LB medium and culture overnight at 30°C in a shaker with a rotation speed of 150rpm to obtain an overnight culture; , 5%, 6%, 7%, 8%, 9%, 10% of the malachite green medium, the initial concentration of malachite green in each medium is 70mg / L. After culturing for 32 hours, the collected samples were centrifuged at 12,000 rpm for 5 minutes, and the supernatant was taken, and the absorbance was measured at 620 nm using a UV-visible spectrophotometer. The decolorization rate (%)=(A-B) / A×100, wherein: A is OD value of the decolorized culture medium before decolorization, and B is the OD value of the decolorized culture medium after decolorization. Such as figure 2As shown, the degradation rate of malachite green with an initial concentration of 70mg / L can be as high as 91% under the condition of 9% salt concentration. It can be seen...

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Abstract

The invention discloses a strain of Enterobacter aerogenes S27 that degrades malachite green, which is preserved in the China Center for Type Culture Collection, with a preservation number of CCTCC NO: M 2017452 and a preservation date of August 28, 2017. The preservation address is Wuhan University, Wuhan, China. The bacteria can tolerate salt, can adapt to harsh environments, has low cost of use, and good ecological restoration. Provides beneficial strain resources.

Description

Technical field: [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to an enterobacter aerogenes isolated from Suaeda viridans, and a method and application thereof for degrading malachite green under high-salt concentration conditions. Background technique: [0002] Malachite Green (Malachite Green, MG), also known as basic green, is a blue-green crystal with metallic flashes and is easily soluble in water. It is a synthetic triphenylmethane industrial dye and has been widely used in silk, wool, Cotton and other dyes, etc. Because malachite green has very obvious effects in killing parasites in fish, preventing saprolegniasis and gill rot, it has been widely used in aquaculture. However, studies have found that the important functional group of malachite green is triphenylmethane, which is a strong carcinogen and has had a significant impact on human society and the natural environment through the food chain. The use of mal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/38
CPCB09C1/10C02F3/34C02F2101/308C02F2101/38C12N1/20C12N1/205C12R2001/01
Inventor 赵国琰尚念杰
Owner SHANDONG NORMAL UNIV
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