Establishment method of silicosis pulmonary fibrosis animal model and special intubation device

A pulmonary fibrosis and animal model technology, applied in the biological field, can solve the problems of low infection probability, short operation time, increase the influence of non-experimental factors, etc., and achieve the effect of reducing mortality and improving the success rate of construction

Inactive Publication Date: 2018-04-06
山东省职业卫生与职业病防治研究院
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invasive intubation method has a high success rate, but it will cause trauma to the rat trachea and surrounding tissues, which increases the influence of non-experimental factors on the experimental results; the

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Establishment method of silicosis pulmonary fibrosis animal model and special intubation device
  • Establishment method of silicosis pulmonary fibrosis animal model and special intubation device
  • Establishment method of silicosis pulmonary fibrosis animal model and special intubation device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Such as figure 1 As shown, the special intubation device for establishing an animal model of silicosis pulmonary fibrosis of the present invention includes an intubation assembly, a detection assembly and a liquid injection part, and the intubation assembly includes an inner tube and an outer tube, and the inner tube is sheathed on the in the outer tube. The material used for the inner tube is metal, and the material used for the outer tube is a plastic soft rubber tube. The soft rubber tube will not damage the trachea of ​​the rat, and the inner tube is made of a rigid metal material that can effectively support the outer tube and prevent it from being inserted. Shrunken, airless.

[0045] A liquid injection part is detachably connected to the opening of the outer tube, and a second valve is installed on the opening to control the opening and closing of the opening. The specific structure of the liquid injection part can adopt structures such as a syringe. The front...

Embodiment 2

[0048] 1. Preparation before experiment

[0049] (1) Tie a circle of gauze on the tissue tweezers to avoid damage to the rat tongue caused by too much force when clamping out the tongue;

[0050] (2) Nail a nail on both sides of the top of the metal bracket similar to the length of the experimental rat, and fix the string on the nail to hook the upper incisors of the rat to fix the rat;

[0051] (3) Inject clean water into the detection component, so that the amount of water in the water pipe is slightly less than 1 / 2, which is used to check whether the intubation tube is correctly inserted into the rat trachea.

[0052] (4) Preparation of SiO 2 Suspension: SiO 2 Dry at 140°C for 2 hours, after grinding, use physiological saline to prepare SiO with a concentration of 50mg / ml 2 The suspension was autoclaved at 121°C for 40 minutes, and shaken well before administration. (5) Preparation of chloral hydrate solution: make 10% solution of chloral hydrate with physiological salin...

Embodiment 3

[0064] The acquisition of embodiment 3 experimental materials

[0065] The mortality rate of the rats after perfusion was observed, and compared with the tracheotomy intubation group and the blind intubation group respectively. After all the surviving rats regained consciousness, they were put back into the cage for feeding. On the 28th day, the rats were anesthetized, the rats were dissected on a fixed board, the rat heart was exposed, a small opening was cut in the right atrial appendage, and a syringe needle was inserted from the left ventricle of the rat, and 120ml of PBS buffer and 4% The paraformaldehyde was used for perfusion, the blood of the rat was washed out of the body, the lung tissue was taken out, washed with 4% paraformaldehyde, fixed, and stored at 4°C. After dehydration, transparent, paraffin embedding, section processing, HE staining and Masson staining.

[0066] Table 1 Comparison data of rat mortality

[0067]

[0068] Conclusion: the number of dead ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to view more

Abstract

The invention relates to an establishment method of a silicosis pulmonary fibrosis animal model and a special intubation device. The establishment method comprises the following steps: (1) narcotizingand fixing a rat, and exposing the glottis of the rat; (2) under the irradiation of a cold light source, inserting an intubation assembly into a tracheae; (3) observing the change situation of liquidlevel of a detection assembly, and judging that the intubation assembly has been inserted into the tracheae if the liquid level fluctuates; and (4) after the intubation assembly has been inserted into the tracheae, injecting SO2 turbid liquid into the rat. The provided method and intubation device has the advantages that by setting a detection assembly, people can judge whether the intubation iscorrect or not, the death rate of the rat is reduced therefore, and the success rate of model construction is increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for establishing an animal model of silicosis and pulmonary fibrosis and a special intubation device. Background technique [0002] Silicosis is a systemic disease mainly caused by lung fibrosis caused by long-term exposure to free silica dust. It is the most common occupational disease. Its incubation period is long and its pathogenesis is complicated. Even if it is removed from the dust environment, the disease can still progress. At present, there is no effective drug and method for treating silicosis, which seriously endangers the physical and mental health of workers. [0003] Animal experiments are often required to explore the pathogenesis of silicosis and develop related therapeutic drugs, and the successful establishment of animal models of silicosis is very important. At present, the commonly used drug administration method for animal models of silicos...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K33/00A61D7/00
CPCA61D7/00A61K33/00
Inventor 邵华杜忠君陈尚雅于功昌姜岩
Owner 山东省职业卫生与职业病防治研究院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap