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Probe composition and application of thereof in detecting lung cancer and colorectal cancer genes based on NGS method

A colorectal cancer, composition technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial assay/inspection, etc., can solve the problems of low cost and high sensitivity, improve possibility, reduce complexity, and solve synthetic problems Effects of cost issues

Inactive Publication Date: 2018-04-06
上海仁东医学检验所有限公司
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  • Abstract
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Problems solved by technology

[0013] In addition, liquid biopsy techniques include digital PCR, ArmsPCR, etc., which have high sensitivity and low cost, and the disadvantage is that only a few known sites can be detected

Method used

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  • Probe composition and application of thereof in detecting lung cancer and colorectal cancer genes based on NGS method
  • Probe composition and application of thereof in detecting lung cancer and colorectal cancer genes based on NGS method
  • Probe composition and application of thereof in detecting lung cancer and colorectal cancer genes based on NGS method

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Embodiment Construction

[0029] The technical solution of the present invention is described in detail below. Those skilled in the art should understand that the technical solution of the invention can be modified or equivalently replaced, which should be covered by the scope of the claims of the present invention.

[0030] In this example, 15 lung cancer-related genes were selected for panel design, such as: EGFR, ALK, KRAS, BRAF, ROS1, RET, ERBB2, MET, AKT1, NRAS, PIK3CA, PTEN, DDR2, MAP2K1 (MEK1) and FGFR1, 12 genes related to colorectal cancer were also selected, such as: EGFR, KRAS, BRAF, ERBB2, MET, AKT1, NRAS, PIK3CA, PTEN, MAP2K1 (MEK1), FGFR1 and SMAD4.

[0031] The verification method adopted in the library building hybridization capture process of the present invention is as follows:

[0032] 1.1gDNA fragmentation and purification:

[0033]1.1.1. Take 500ng according to the qubit concentration, add water to make up to 100μl, add covaris 130μl interrupted tube, set the program: 50W, 20%, 2...

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Abstract

The invention discloses a probe composition which comprises probes composed of DNA sequences shown as SEQ ID No 1 to SEQ ID No 618. The probe composition provided by the invention can be used for a molecular diagnostic technique for performing precise treatment on patients suffering from lung cancer and colorectal cancer, supports liquid biopsy and has the sensitivity of 0.1%.

Description

technical field [0001] The invention relates to a cancer gene detection composition, in particular to a DNA sequence probe composition, and its application in the detection of lung cancer and colorectal cancer genes based on NGS methods. Background technique [0002] High-throughput sequencing technology performs sequence determination on hundreds of thousands to several million DNA molecules at a time. It is possible to conduct a detailed and comprehensive analysis of the transcriptome and genome of a species, so it is also called Deep Sequencing (Deep Sequencing). The Illumina platform is now the mainstream next-generation sequencing platform, with a market share of over 90%. [0003] Compared with early gene detection technologies: Fluorescence in situ hybridization (FISH) has high sensitivity but is difficult to operate; traditional polymerase chain reaction (PCR) has advantages in ease of operation and rapidity, but technical principles limit It can only detect one or...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6869C12N15/11
CPCC12Q1/6869C12Q1/6886C12Q2600/166C12Q2535/122
Inventor 徐小红曹建军赵国栋
Owner 上海仁东医学检验所有限公司
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