A method for identifying the tissue origin of human body fluids and its dedicated miRNA panel
A body fluid and source technology, applied in the field of forensic technology, can solve the problems of difficult detection, small molecular weight, small molecules, etc., and achieve the effect of improving scientificity and accuracy
Active Publication Date: 2021-06-29
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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Problems solved by technology
[0003] miRNA is a kind of endogenous non-coding small molecular RNA composed of about 18-25 nucleic acids that widely exists in eukaryotic cells. Its content and molecular structure have no obvious changes under the conditions of strong acid and strong alkali, which meets the needs of detecting degraded samples, and has become a hot spot for forensic science after mRNA; but its high copy number and small molecular weight make it It is difficult to detect
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Embodiment 1
[0051] Example 1. Method for identifying the tissue source of human body fluid based on the relative expression levels of six miRNAs
[0052] 1. Sample collection
[0053] 105 samples came from 50 volunteers (aged 23-35) in Beijing area. 50 volunteers were unrelated Han individuals, and all of them gave informed consent. 40 peripheral blood samples were collected from the venous blood of some volunteers, 31 saliva samples were collected from volunteers in centrifuge tubes, and 18 menstrual blood samples were collected from volunteers on the 2nd or 2nd day of menstruation with sterile cotton swabs. On the 3rd day, 16 semen were obtained by collecting fresh semen from volunteers in a semen collection cup.
[0054] All 105 samples were stored at -80°C.
[0055] 2. Acquisition of cDNA
[0056] 1. Use miRNeasy Mini Kit to extract the Total RNA of 105 samples respectively, and then use TurboDNA-free TM The kit was purified (for the purpose of removing genomic DNA), and the Tot...
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The invention discloses a method for identifying the tissue source of human body fluid. The method includes detecting the expression levels of miRNA205, miRNA451, miRNA144, miRNA214, miRNA888 and miRNA891 in body fluid, and then using the softmax regression model and the one-hot form of classification to calculate the probability value that the body fluid to be tested comes from the tissue source, and the probability value is the largest is the tissue source of the body fluid. The method provided by the present invention can effectively identify the tissue source of body fluid, greatly simplifies the steps of identifying the tissue source of body fluid or its spots at the scene of criminal investigation, and can provide accurate scientific evidence for clarifying the nature of the case, determining the criminal suspect, and convicting and sentencing. in accordance with.
Description
technical field [0001] The invention relates to the field of forensic technology, in particular to a method for identifying the tissue source of human body fluid and a special miRNA combination thereof. Background technique [0002] Identifying the tissue source of human body fluids (such as blood, semen spots, saliva) left at the crime scene is of great significance for the qualitative case or reconstruction of the crime scene. Therefore, identifying the tissue source of body fluids has always been an important research content of forensic evidence. Traditional identification methods for tissue sources of body fluids are mainly based on enzymatic reactions or immunological applications, but the identification process is cumbersome, and good identification results are often not obtained for trace body fluid samples that usually appear in criminal cases. At present, the identification method of the tissue source of body fluid mainly relies on the means of molecular biology, s...
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IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/158C12Q2600/178
Inventor 孙启凡季安全胡胜李冉冉江丽李彩霞王乐
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY