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Method rapidly cultivating deciduous holly tissue cultured seedlings

A technology of deciduous holly and tissue culture seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of limited promotion and reproduction, low multiplication coefficient of seedlings, and low rooting rate, and achieve strong promotion and application value , Accelerate the reproduction speed and scale, and grow robustly

Inactive Publication Date: 2018-05-15
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low multiplication coefficient, slow rooting and low rooting rate of deciduous holly tissue culture seedlings, its large-scale promotion and propagation are limited.

Method used

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  • Method rapidly cultivating deciduous holly tissue cultured seedlings
  • Method rapidly cultivating deciduous holly tissue cultured seedlings
  • Method rapidly cultivating deciduous holly tissue cultured seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for quickly cultivating Ilex deciduous tissue culture seedlings, the kind of Ilex deciduous is: 'Winter Red', comprising the following steps:

[0032] 1) Transfer the axillary buds induced by conventional methods into the proliferation medium, and after 25 days, a small amount of clustered buds can be produced, and then use 25 days as the subculture cycle, and then transfer the bud clusters with 5-6 small buds into the proliferation medium In the culture medium, a large number of clustered buds can be obtained, and the proliferation rate is about 425%. Among them, the proliferation medium is: MS+0.5 mg / L NAA+0.5 mg / L 6-BA+1.0 mg / L CPPU. The medium contains 3.0% sucrose, 0.65% agar, and the pH is about 5.8. The temperature of the culture room is 25±2°C, the light time is 14h / d, and the light intensity is 55 μmol m –2 ·s –1 ( figure 1 ).

[0033] 2) Transfer the deciduous holly cluster buds after proliferation culture into the strong seedling medium. After ...

Embodiment 2

[0038] A method for quickly cultivating and obtaining Ilex deciduous tissue culture seedlings, the kind of Ilex deciduous is: 'Red Sprite', comprising the following steps:

[0039] 1) Transfer the axillary buds induced by conventional methods into the proliferation medium, and after 25 days, a small amount of clustered buds can be produced, and then use 25 days as the subculture cycle, and then transfer the bud clusters with 5-6 small buds into the proliferation medium In the culture medium, a large number of clustered buds can be obtained, and the proliferation rate is 415%. The proliferation medium is: MS+0.1 mg / L NAA+0.5 mg / L 6-BA+0.5mg / L CPPU+1.0 mg / LGA. The medium contains 3.0% sucrose, 0.65% agar, and the pH is about 5.8. The temperature of the culture room is 25±2°C, the light time is 14h / d, and the light intensity is 55 μmol m –2 ·s –1 ( Figure 5 ).

[0040] 2) Transfer the deciduous holly cluster buds after proliferation culture into the strong seedling medium. Af...

Embodiment 3

[0045] A method for quickly cultivating and obtaining Ilex deciduous tissue culture seedlings, the kind of Ilex deciduous is: 'Winter Gold', comprising the following steps:

[0046] 1) Transfer the axillary buds induced by conventional methods into the proliferation medium, and after 25 days, a small amount of clustered buds can be produced, and then use 25 days as the subculture cycle, and then transfer the bud clusters with 5-6 small buds into the proliferation medium In the culture medium, a large number of clustered buds can be obtained, and the proliferation rate is 446%. Proliferation medium is: MS+0.5mg / L NAA+0.2 mg / L 6-BA+0.5 mg / L CPPU. The medium contains 3.0% sucrose, 0.65% agar, and the pH is about 5.8. The temperature of the culture room is 25±2°C, the light time is 14h / d, and the light intensity is 55 μmol m –2 ·s –1 .

[0047] 2) Transfer the deciduous holly cluster buds after proliferation culture into the strong seedling medium. After 25 days of cultivation...

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Abstract

The invention discloses a method for rapidly cultivating deciduous holly tissue cultured seedlings. The method comprises the following steps: firstly, performing primary culture on axillary buds whichare subjected to a conventional disinfection and inoculation method, so as to obtain 1.5 to 2.0cm germ-free buds; cutting and transferring the germ-free buds into an enrichment medium, firstly performing culture to produce a small quantity of cluster buds, and then performing secondary culture, so as to obtain a large quantity of cluster buds; secondly, transferring deciduous holly clumpy buds subjected to enrichment culture into a strong seedling medium, performing culture for 25d, then performing multiplication on the clumpy buds, and ensuring that the quantity of effective buds is increased; thirdly, scissoring strong buds which is greater than 3.0cm, inoculating the strong buds into a rooting induction medium, ensuring that rooting is performed 15 to 25d after the inoculation, performing culture for 35 to 45d, and then ensuring that regeneration plants are grown to greater than 5.0 to 6.0cm, and young seedlings are robust; fourthly, performing tempering seeding, acclimatizating and transplanting on the young seedlings when the young seedlings to be rooted are grown to greater than 5.0 to 6.0cm and the quantity of the young seedlings to be rooted is 8 to 10. The method is simple and convenient to operate, multiplication and strong seedling are performed for 75 days, root induction is advanced to 15 to 25 days, the maximum multiplication rate of bud seedlings reaches 415 to446 percent, the rooting rate reaches 65 to 80 percent, and the transplanting survival rate reaches 85 to 90 percent or more.

Description

technical field [0001] The invention relates to the technical field of tissue culture of deciduous holly, in particular to a method for rapidly cultivating deciduous holly tissue culture seedlings. Background technique [0002] Deciduous holly ( Ilex verticillata L.) is a deciduous shrub of the holly family Ilex introduced from eastern North America. It is dioecious and is usually distributed around swamps and ponds. There are many cultivars. The deciduous type holly is different from other evergreen holly species. It is characterized by falling leaves in winter, and bright and full red fruits hang all over the branches. The fruit-bearing period can last until April of the following year. One of the best trees for fruit. It can be used not only for commercial branching but also for garden greening, indoor bonsai, and garden viewing in winter. It has great garden viewing and commercial value. Deciduous holly has not been introduced to my country for a long time, and the n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈颖陈茜陈燕琼袁斌玲魏子涵
Owner NANJING FORESTRY UNIV
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