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Application of AMP-activated protein kinase (AMPK) as biomarker of tumor metastasis and prognosis diagnosis

A technology of biomarkers and activated proteins, applied in the field of molecular diagnosis, can solve problems such as unclear

Inactive Publication Date: 2018-05-15
SICHUAN UNIV
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Problems solved by technology

However, whether AMPK gene expression has significant changes in clinical tumors and whether AMPK plays a role in the process of tumor cell deterioration and metastasis is still unclear.

Method used

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  • Application of AMP-activated protein kinase (AMPK) as biomarker of tumor metastasis and prognosis diagnosis
  • Application of AMP-activated protein kinase (AMPK) as biomarker of tumor metastasis and prognosis diagnosis
  • Application of AMP-activated protein kinase (AMPK) as biomarker of tumor metastasis and prognosis diagnosis

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Experimental program
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Embodiment

[0050] (1) The mRNA expression level of AMPKα1 decreased significantly with the increase of the malignancy of breast cancer

[0051] AMPK consists of three subunits: including catalytic subunit α, connecting subunit β and binding subunit γ. In order to study the changes of AMPK in tumor cells, the inventors analyzed the mRNA expression of AMPK catalytic subunit α1 in different types of breast cancer through clinical samples. Such as Figure 1A-Figure 1C As shown, in different types of breast cancer: including triple-negative breast cancer (TNBC), Her2+ / PR- / ER- breast cancer, the mRNA level of AMPKα1 decreased significantly with the increase of tumor malignancy. It is suggested that AMPK plays an important role in the occurrence and development of breast cancer tumors.

[0052] (2) The protein expression level of AMPKα1 decreased significantly with the increase of the malignancy of breast cancer

[0053] On the basis of (1), the expression of AMPKα1 in 27 breast cancer tissu...

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Abstract

The invention belongs to the technical field of molar-diagnosis and solves the problems that in the prior art, deterioration and metastasis of tumor cells are not known clearly and diagnosis evidencecannot be supplied to tumor metastasis and prognosis diagnosis. The invention provides an application of an AMP-activated protein kinase (AMPK) as a biomarker of tumor metastasis and prognosis diagnosis, and an application of AMPK[alpha]1 as a biomarker for the tumor metastasis and prognosis diagnosis. In the invention, an experiment proves that cancer signals (PI3K, Ras and Her2) inhibiting protein expression of the AMPK[alpha]1 in malignant tumors are matched with clinical data very well, thus proving that the significant low expression of the AMPK[alpha]1 in malignant tumors is a key factorof the metastasis of malignant tumors; with the gene expression level of the AMPK[alpha]1 as an important index for the diagnosis and prognosis of tumor metastasis, the AMPK[alpha]1 can be applied asa biomarker to the tumor metastasis and prognosis diagnosis.

Description

technical field [0001] The invention belongs to the technical field of molecular diagnosis, and specifically relates to the use of adenylate-activated protein kinase AMPK as a biomarker for tumor metastasis and prognosis diagnosis. Background technique [0002] With the increasing incidence and mortality of cancer, cancer has become one of the leading causes of chronic disease death in the world population. Tumor metastasis is one of the main causes of cancer death. Large clinical data show that a large number of cancer patients have metastasized at the stage of small tumor size in situ, but effective treatment is delayed because it is difficult to detect. At present, there is a lack of molecular diagnostic products for predicting tumor metastasis and effective prognosis assessment on the market. Therefore, the development of molecular detection technologies and products for tumor metastasis has great clinical significance. [0003] Adenylate-activated protein kinase (AMPK...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57415G01N2333/91215
Inventor 肖智雄易勇
Owner SICHUAN UNIV
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