A kind of rooting induction medium of Limonium chrysalis and breeding method of Limonium chrysalis

Abstract

The invention provides a rooting induction culture medium for limonium aureum and belongs to the technical field of tissue culture. A 1 / 2-LS culture medium is used as a basic culture medium. The culture medium comprises 2.0-5.0 mg / L zeatin, 0.5-1.5 mg / L alpha-naphthylacetic acid, 3.0-5.0 g / L activated carbon,10-20 g / L sucrose and 6.0-7.0 g / L agar; the pH value of the rooting induction culture medium is 5.0-6.5. According to the rooting induction culture medium for limonium aureum, the root system can be quickly generated, roots and seedlings grow robustly, and the rooting rate can reach 90-95%.

Description

technical field

[0001] The invention relates to the technical field of tissue culture, in particular to a rooting induction medium for Limonium chrysalis and a breeding method for Limonium chrysalis. Background technique

[0002] Limonium chrysalis is a perennial herb of the genus Limonium genus in the family Lemonaceae. It is resistant to salt and alkali, barrenness, and drought. It grows mostly in dry desert areas such as beaches, Gobi, rocky hillsides, and mobile sand dunes. It is mainly distributed in Hulunbeier Sandy Land, Hunshan Dake Sandy Land, Mu Us Sandy Land, Ulan Buhe Desert, Tengger Desert, and Hexi Corridor in Gansu Province; in areas with an annual rainfall of more than 300 mm, it can grow normally without watering, which is 1 / 10 of the water consumption of lawns It can grow and bloom normally when the soil pH is below 9 and the salt content is within 0.4%. What is particularly peculiar is that its membranous calyx does not fall during the windless season from...

Examples

Embodiment 1

[0063] 1. Main steps

[0064] 1. Seed disinfection treatment:

[0065] Select a number of plump and uniform-sized Limonium chrysalis seeds harvested in the same year, and place them in a container with a sealed lid. The volume of the container is about 20ml. Shake vigorously for 10 minutes, soak in water for 10 hours, and then use a pipette to absorb the liquid. Remove and rinse once with water to obtain rinsed seeds.

[0066] Rinse the seeds for disinfection first soak in 15% sodium hypochlorite for 10 minutes, then rinse with sterile water for 3 times; then soak in 70% ethanol for 40 seconds, rinse with sterile water for 3 times; Rinse with water for 5 times to obtain sterilized seeds; use the above reagents to soak and cover the plastic tube after rinsing with sterile water, and then shake the plastic tube continuously to make the seed disinfection treatment more thorough and sufficient. Finally, use tweezers to clamp out the treated seeds and place them Store in a Petri ...

Embodiment 2

[0080] 1. Main steps

[0081] 1. Seed disinfection treatment:

[0082] Select a few plump and uniform-sized Limonium chrysalis seeds harvested in the same year, and place them in a container with a sealed lid. The volume of the container is about 20ml. Shake vigorously for 10 minutes, soak in water for 11 hours, and then use a pipette to absorb the liquid. Remove and rinse once with water to obtain rinsed seeds.

[0083] Rinse the seeds for disinfection first soak in 20% sodium hypochlorite for 7 minutes, then rinse with sterile water for 3 times; then soak in 75% ethanol for 25 seconds, rinse with sterile water for 4 times; then soak in 0.15% mercury chloride for 2 minutes, and then rinse with sterile Rinse with water for 5 times to obtain sterilized seeds; use the above reagents to soak and cover the plastic tube after rinsing with sterile water, and then shake the plastic tube continuously to make the seed disinfection treatment more thorough and sufficient. Finally, use t...

technical field

[0001] The invention relates to the technical field of tissue culture, in particular to a rooting induction medium for Limonium chrysalis and a breeding method for Limonium chrysalis. Background technique

[0002] Limonium chrysalis is a perennial herb of the genus Limonium genus in the family Lemonaceae. It is resistant to salt and alkali, barrenness, and drought. It grows mostly in dry desert areas such as beaches, Gobi, rocky hillsides, and mobile sand dunes. It is mainly distributed in Hulunbeier Sandy Land, Hunshan Dake Sandy Land, Mu Us Sandy Land, Ulan Buhe Desert, Tengger Desert, and Hexi Corridor in Gansu Province; in areas with an annual rainfall of more than 300 mm, it can grow normally without watering, which is 1 / 10 of the water consumption of lawns It can grow and bloom normally when the soil pH is below 9 and the salt content is within 0.4%. What is particularly peculiar is that its membranous calyx does not fall during the windless season from...

Examples

Embodiment 1

[0063] 1. Main steps

[0064] 1. Seed disinfection treatment:

[0065] Select a number of plump and uniform-sized Limonium chrysalis seeds harvested in the same year, and place them in a container with a sealed lid. The volume of the container is about 20ml. Shake vigorously for 10 minutes, soak in water for 10 hours, and then use a pipette to absorb the liquid. Remove and rinse once with water to obtain rinsed seeds.

[0066] Rinse the seeds for disinfection first soak in 15% sodium hypochlorite for 10 minutes, then rinse with sterile water for 3 times; then soak in 70% ethanol for 40 seconds, rinse with sterile water for 3 times; Rinse with water for 5 times to obtain sterilized seeds; use the above reagents to soak and cover the plastic tube after rinsing with sterile water, and then shake the plastic tube continuously to make the seed disinfection treatment more thorough and sufficient. Finally, use tweezers to clamp out the treated seeds and place them Store in a Petri ...

Embodiment 2

[0080] 1. Main steps

[0081] 1. Seed disinfection treatment:

[0082] Select a few plump and uniform-sized Limonium chrysalis seeds harvested in the same year, and place them in a container with a sealed lid. The volume of the container is about 20ml. Shake vigorously for 10 minutes, soak in water for 11 hours, and then use a pipette to absorb the liquid. Remove and rinse once with water to obtain rinsed seeds.

[0083] Rinse the seeds for disinfection first soak in 20% sodium hypochlorite for 7 minutes, then rinse with sterile water for 3 times; then soak in 75% ethanol for 25 seconds, rinse with sterile water for 4 times; then soak in 0.15% mercury chloride for 2 minutes, and then rinse with sterile Rinse with water for 5 times to obtain sterilized seeds; use the above reagents to soak and cover the plastic tube after rinsing with sterile water, and then shake the plastic tube continuously to make the seed disinfection treatment more thorough and sufficient. Finally, use t...

Images