11 results about "Limonium vulgare" patented technology

The invention discloses a method for guiding light rare earth surface mine degraded plant community restoration through combination of limonium bicolor, mycorrhizal fungi and tolerant earthworm cast, and the method utilizes a combination mode of limonium bicolor, mycorrhizal fungi and tolerant earthworm cast to guide and construct a plant community-soil fungi-soil ecosystem circulation system. According to the method, the existing plant community of the light rare earth mine is guided to recover by utilizing a combination method of combining dominant plant species with different mycorrhizal fungi and adding tolerance earthworm manure, mycorrhizal fungi and the earthworm manure fertilizer are added in the transplanting process of adult plants, so that the plant survival is promoted, and the stress resistance of light rare earth is enhanced at the same time; plant-mycorrhiza-wormcast fertilizer combinations aiming at different light rare earth elements are screened out, and the whole plant community of the mine is recovered and the content of the light rare earth elements in the soil is reduced through a plant community recovery method.

The invention belongs to the technical field of biology, and particularly relates to application of LbSNAP331 in salt resistance of plants. Specifically, a gene highly responsive to salt stress is screened from an LbSNARE gene family of limonium bicolor, and the gene is named as LbSNAP331. Researches show that the gene is positioned on salt glands, and the gene regulates the number of the salt glands and the secretion rate of a single salt gland, reduces the Na < + >, Na < + > / K < + > content and active oxygen content of the limonium bicolor and resists oxidative stress, so that the salt resistance and salt gland development conditions of the limonium bicolor are positively regulated. The invention lays a foundation for explaining the plant salt resistance mechanism and improving the utilization efficiency of the saline-alkali soil, so that the application prospect is wide.

The invention discloses a limonium bicolor gene LbZFP8 and application thereof, and relates to the technical field of biological genetic engineering. The invention relates to a limonium bicolor gene LbZFP8 and application thereof. The limonium bicolor gene LbZFP8 is a gene for coding a protein composed of an amino acid sequence as shown in SEQ ID NO: 1; the biological material is recombinant DNA, an expression cassette, a transposon, a plasmid vector, a virus vector or engineering bacteria. The gene provided by the invention can significantly improve the salt tolerance and oxidation resistance of a target plant by transforming plant cells. Experiments show that under the salt stress condition, the survival rate of the LbZFP8 overexpressed arabidopsis thaliana plant is increased by about 45% compared with that of a control group. Meanwhile, the compound plays a key role in regulating and controlling an active oxygen scavenging system, and the activity of SOD and POD enzymes can be effectively enhanced. The technology provides an important gene resource for cultivating new varieties of crops with strong stress resistance, and has a wide application prospect.

The invention relates to the technical field of plant genetic engineering, and discloses a limonium bicolor gene LbYAB1 and application thereof. The new function of the LbYAB1 is found, firstly, the expression of the LbYAB1 in salt glands and leaf primordia is proved through RNA in-situ hybridization and a promoter driven GUS tissue staining technology, and the expression of the LbYAB1 is induced by treatment of 6-benzylaminopurine. Secondly, a genetic transformation technology is updated, a stable overexpressed LbYAB1 strain is obtained, and by exploring the LbYAB1 function of an overexpressed and silent strain, it is found that compared with a wild type, development of salt glands of the overexpressed strain is inhibited, the salt secreting capacity is weakened, and the salt tolerance is reduced; a silent strain shows an opposite phenotype, and a result proves that the LbYAB1 is a negative regulation factor for salt gland development. Overexpression of the gene in limonium bicolor and arabidopsis thaliana both leads to enhancement of salt sensitivity. The invention not only lays a foundation for further exploring and revealing the development mechanism of salt glands, but also opens up a new path for cultivating salt-tolerant crops.

The application discloses a culture medium for tissue culture of Limosella australis, which comprises a primary culture medium, a proliferation and differentiation culture medium and a rooting culture medium. The primary culture medium is MS+6BA 0.4-0.6 mg / L+NAA 0.04-0.06 mg / L+AC 0.8-1.2 mg / L+2,4-D 0.04-0.06 mg / L. The proliferation and differentiation culture medium is modified MS+6BA 0.09-0.11 mg / L+NAA 0.04-0.06 mg / L+AC 1.5-2.5 mg / L. The rooting culture medium is 1 / 2MS+IAA 0.09-0.11 mg / L+AC 2.5-3.5 mg / L. The technical scheme has the highest callus induction rate of 86.7%, the highest differentiation rate of 86.7% and the highest rooting rate of 93.3%.

The invention relates to the technical field of chemical treatment of natural plants, in particular to a preparation method of an antioxidant feed additive, which comprises the following steps: taking limonium aureum as a raw material, air-drying and crushing the raw material to obtain a crushed bulk drug; adding the crushed raw material medicines into a solvent, and performing ultrasonic extraction, concentration, alcohol precipitation and centrifugation to obtain limonium aureum polysaccharide, namely the anti-oxidation limonium aureum feed additive; performing a single-factor test according to four factors and five levels, and respectively investigating the influence of the extraction temperature X1, the ultrasonic power X2, the ultrasonic time X3 and the material-liquid ratio X4 on the yield of the polysaccharide in the limonium aureum; on the basis of a single factor level, Box-Behnken response surface design is carried out, and a test is carried out according to a factor level combination; and carrying out analysis on the test data by adopting Design-Expert 8.0. 6 statistical analysis software. The preparation method of the antioxidant feed additive provided by the invention has the beneficial effects that the content of polysaccharide in the antioxidant feed additive reaches 32.26%.

This invention relates to the field of plant genetic engineering, specifically to the LbMYB75 gene of *Limonium bicolor* and its applications. This invention discloses that the transcription factor LbMYB75 is specifically highly expressed in the early stages of salt gland development and is significantly induced by salt. This invention confirms that the LbMYB75 gene positively regulates salt gland development and salt secretion in *Limonium bicolor*. Salt gland density is significantly increased in *Limonium bicolor* lines overexpressing the LbMYB75 gene, while salt gland density is significantly decreased in LbMYB75 mutant lines. The transcription factor LbMYB75 positively regulates the salt tolerance of *Limonium bicolor*; compared with the wild type, the overexpressing lines under high salt conditions show higher levels of malondialdehyde (MDA) and sodium. + and Na + / K + K decreased significantly. + Increased proline; This invention elucidates the regulatory role of the LbMYB75 transcription factor in salt gland development of *Limonium bicolor*, providing genetic resources and theoretical basis for improving the salt tolerance of non-halophytes through transgenic means, and contributing to the development and utilization of saline-alkali soils.

The invention provides a traditional Chinese medicine composition for treating renal proteinuria as well as a preparation method and application thereof, and particularly belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 30-60 parts of radix astragali, 10-20 parts of roasted rhizoma atractylodis macrocephalae, 8-16 parts of rhizoma polygoni cuspidati, 8-16 parts of rhizoma alismatis, 10-20 parts of rhizoma smilacis glabrae, 8-16 parts of rhizoma curcumae longae, 8-16 parts of lumbricus, 8-16 parts of silkworm larva, 10-20 parts of herba lysimachiae, 10-20 parts of herba centellae, 10-20 parts of sticktight and 10-20 parts of limonium bicolor. The traditional Chinese medicine composition can effectively relieve proteinuria, protect the renal function and regulate the expression of kidney inflammation macrophages, and particularly has the effects of promoting the expression of anti-inflammatory macrophages, reducing the inflammatory factor level of kidney tissue and inhibiting an inflammation signal channel. The traditional Chinese medicine composition provides a safe and effective medicine for treating proteinuria for patients.

The invention discloses a culture medium for tissue culture of limonium bicolor. The culture medium comprises a primary culture medium, a proliferation and differentiation culture medium and a rooting culture medium, the primary culture medium is prepared from MS, 0.4 mg / L to 0.6 mg / L of 6BA, 0.04 mg / L to 0.06 mg / L of NAA, 0.8 mg / L to 1.2 mg / L of AC and 0.04 mg / L to 0.06 mg / L of 2, 4-D; the proliferation and differentiation culture medium is prepared from improved MS, 0.09 mg / L to 0.11 mg / L of 6BA, 0.04 mg / L to 0.06 mg / L of NAA and 1.5 mg / L to 2.5 mg / L of AC; the rooting culture medium is prepared from 1 / 2 MS, 0.09 mg / L to 0.11 mg / L of IAA and 2.5 mg / L to 3.5 mg / L of AC; by using the technical scheme provided by the invention, the highest callus induction rate is 86.7%, the highest differentiation rate is 86.7%, and the highest rooting rate is 93.3%.

The present application belongs to the field of agricultural microorganism technology, and particularly relates to a salt-tolerant growth-promoting bacterium and application of the bacterium in promoting growth of wheat under salt stress. Bacillus cereus The present application is named as Bacillus cereus, and is referred to as strain STGP1 for short, is preserved in the China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No.50114 and a preservation date of August 19, 2026. The strain STGP1 is isolated from rhizosphere soil of two-colored Limonium gmelinii in Dongying saline-alkali soil through enrichment culture and plate streaking technology. The present application has excellent salt tolerance and multiple growth-promoting characteristics. Potted and field tests prove that the strain STGP1 can significantly promote growth of wheat under salt stress and improve the yield of wheat in saline-alkali soil.

This invention relates to the field of plant tissue culture technology, specifically a method for culturing *Limonium bicolor* tissue, comprising five steps: explant preparation and sterilization, callus induction culture, adventitious shoot proliferation culture, rooting culture, and hardening-off transplanting. Modified polyvinylpyrrolidone (PVP) and modified chitosan form a composite material through borate ester bonds, which accumulates around the explant. MES maintains pH stability, allowing PPVP to continuously diffuse into the wound. Modified chitosan blocks oxygen from direct contact with the wound surface, protecting endogenous phenols; its catechol groups chelate the PPO cofactor Cu. 2+ Activated carbon adsorbs free phenolic substrates in the culture medium, reducing quinone formation at its source. Modified polyvinylpyrrolidone inhibits PPO catalytic activity; grafted ascorbic acid can reduce already formed o-quinones back to catechol, directly removing brown pigment precursors.