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Limonium sinense(Girard)O.Kuntze extract and preparation method and application thereof

A technology of Limonium Limonium and its extract, which is applied in the field of Limonium Limonium extract and its preparation, which can solve the problems of skin immunity imbalance, skin damage, and reduction of skin antioxidant defense ability, and achieve the effect of simple preparation method and prevention of skin aging

Inactive Publication Date: 2016-11-23
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the human body has an antioxidant defense system that can maintain the balance between oxidation and anti-oxidation to slow down the production of active oxygen and free radicals, if the body is exposed to the sun for a long time, the free radicals produced in the human body will be damaged. Causes the skin's antioxidant defense ability to decrease, causing skin damage, such as photoaging, epidermal wrinkles, skin immune disorders, etc.

Method used

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  • Limonium sinense(Girard)O.Kuntze extract and preparation method and application thereof
  • Limonium sinense(Girard)O.Kuntze extract and preparation method and application thereof
  • Limonium sinense(Girard)O.Kuntze extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of Limonium Limonium Extract

[0029] 100g commercially available whole plant of Limonium Limonium (Limonium sinense (Girard) Kuntze) flower, stem leaf and root) is dried and pulverized, mixed with 2000mL 70% ethanol solution (v / v, all in the present invention ethanol solution (both volume concentrations) were mixed and soaked, ultrasonically assisted to extract for 1 hour, filtered, and the filtrate was desolventized under reduced pressure to obtain 20 g of Limonium Limonium crude extract.

[0030] Take the crude extract of Limonium Limonium, dissolve it in deionized water, load it on a commercially available AB-8 resin-packed column, and successively wash it with deionized water, 20% ethanol, 40% ethanol, 60% ethanol, 80% ethanol and 95% ethanol Ethanol gradient elution (percentage is volume percentage), collect eluent respectively, remove ethanol and water under reduced pressure, weigh, obtain water elution part 11g, 20% ethanol elution part 2.0g, 40% ethan...

Embodiment 2

[0032] Determination of total flavonoids content in Limonium Limonica Extract

[0033] With the Limonium Limonium extract obtained in Example 1, according to the method of the Pharmacopoeia of the People's Republic of China (2010 edition), with rutin as a standard substance, the total flavonoid content in the Limonium Limonium extract was determined.

[0034] Determination steps are: 1) Standard curve making: Accurately measure standard solution 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, put in 25ml measuring bottle respectively, add 30% ethanol to 6.0ml, add 5% sodium nitrite Solution (mass percentage concentration) 1ml. Mix well, stand for 6 minutes, add 1ml of 10% aluminum nitrate solution (mass percentage concentration), shake well, stand for 6 minutes. Add 10ml of sodium hydroxide test solution, add 30% ethanol to the scale, shake well, let stand for 15 minutes, use the corresponding reagent as a blank, measure the absorbance at a wavelength of 510nm by UV-visible spectrophotometry, ...

Embodiment 3

[0040] Detection of tyrosinase inhibitory activity

[0041] The Limonium Limonium extract prepared in Example 1 at each elution site was completely dissolved in deionized water, and formulated into a 1.0 mg / mL aqueous solution, i.e. a sample solution, for the determination of tyrosinase inhibitory activity, with arbutin as a reference thing.

[0042] The measurement steps are: mix 70 μL of phosphate buffer solution (pH6.8, 30 mM), 80 μL of 1.66 mM tyrosine solution, and 80 μL of sample solution, incubate in a constant temperature water tank at 37°C for more than 5 minutes, add tyrosinase solution (125 U / ml) 10 μL, after constant temperature at 37°C for 10 minutes, measure the absorbance A at 475nm wavelength with a microplate reader 475 . The sample aqueous solution was replaced with deionized water, and the absorbance was also measured as a reference solution, and the results are shown in Table 2.

[0043] The formula for calculating the inhibition rate of tyrosinase acti...

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Abstract

The invention discloses a limonium sinense(Girard)O.Kuntze extract and a preparation method and application thereof. The preparation method of the limonium sinense(Girard)O.Kuntze extract comprises the following steps that 1, limonium sinense(Girard)O.Kuntze is mixed with a solvent, extraction and filtration are conducted, the solvent is removed through pressure reduction, and a limonium sinense(Girard)O.Kuntze crude extract is obtained; 2, after the limonium sinense(Girard)O.Kuntze crude extract is dissolved in water and then loaded on a resin packed column, elution is conducted with water and an ethanol solution in sequence, eluents are collected respectively, ethanol and the water are removed through pressure reduction, and the limonium sinense(Girard)O.Kuntze extract is obtained. The preparation method of the limonium sinense(Girard)O.Kuntze extract is simple, the obtained limonium sinense(Girard)O.Kuntze extract can be applied to serve as a tyrosinase inhibitor, an elastic protease inhibitor, an antioxidant activator and an anti-ageing activator; preferably, application of the limonium sinense(Girard)O.Kuntze extract in preparing skin care products or cosmetics is achieved.

Description

technical field [0001] The invention relates to the field of plant extraction, in particular to an extract of Limonium Limonium and its preparation method and application. Background technique [0002] In 1956, Harman proposed the theory of free radical aging [Harman, D. Aging: A theory based on free radical and radiation chemistry. J. Gerontol. 1956, 11: 289-300.], which believed that excessive free radicals were the cause of biological aging important reason. According to this theory, excess active oxygen free radicals in the body react with unsaturated fatty acids to produce substances such as malondialdehyde, and malondialdehyde will react with proteins on the cell membrane to produce brown pigment, which precipitates on the skin and becomes various stains. Excessive free radicals can also cause cross-linking and denaturation of collagen fibers and elastic fibers in the skin, making them brittle and losing elasticity. When the skin is insufficiently hydrated, it is easy...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P17/18A61K8/97A61Q19/08
Inventor 方兆华沈忠海宋肖洁摩根·多斯桑托斯李慧黄晓青
Owner JALA GROUP CORPORATION
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