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Plant extract composition and application thereof

A technology of plant extracts and compositions, which is applied in the direction of dressing preparations, cosmetics, skin care preparations, etc., which can solve problems such as skin immunity disorders, skin damage, and reduced skin antioxidant defense capabilities, so as to prevent skin aging , the effect of simple preparation method

Inactive Publication Date: 2016-11-23
JALA GROUP CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the human body has an antioxidant defense system that can maintain the balance between oxidation and anti-oxidation to slow down the production of active oxygen and free radicals, if the body is exposed to the sun for a long time, the free radicals produced in the human body will be damaged. Causes the skin's antioxidant defense ability to decrease, causing skin damage, such as photoaging, epidermal wrinkles, skin immune disorders, etc.

Method used

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  • Plant extract composition and application thereof
  • Plant extract composition and application thereof
  • Plant extract composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation of Limonium Limonium Extract

[0034] 100g commercially available Limonium limonium flower and whole grass (Limonium sinense (Girard) Kuntze) are dried and pulverized, mixed with 2000mL 70% ethanol solution (v / v, all ethanol solutions in the present invention are volume concentration ) were mixed and soaked, ultrasonically assisted for 1 hour, filtered, and the filtrate was desolvated under reduced pressure to obtain 20 g of Limonium Limonium crude extract.

[0035] Take the crude extract of Limonium Limonium, dissolve it in deionized water, load it on the AB-8 resin-filled column, and successively use deionized water, 20% ethanol, 40% ethanol, 60% ethanol, 80% ethanol and 95% ethanol gradient Elution (percentage is volume percentage), collect eluent respectively, remove ethanol and water under reduced pressure, weigh, obtain water elution part 11g, 20% ethanol elution part 2.0g, 40% ethanol elution part 2.2 g, 2.1 g of 60% ethanol elution fraction, 1.1 g o...

Embodiment 2

[0037] Determination of total flavonoids content in Limonium Limonica Extract

[0038] With the Limonium Limonium extract obtained in Example 1, according to the method of the Pharmacopoeia of the People's Republic of China (2010 edition), with rutin as a standard substance, the total flavonoid content in the Limonium Limonium extract was determined.

[0039] Determination steps are: 1) Standard curve making: Accurately measure standard solution 1ml, 2ml, 3ml, 4ml, 5ml, 6ml, put in 25ml measuring bottle respectively, add 30% ethanol to 6.0ml, add 5% sodium nitrite Solution (mass percentage concentration) 1ml. Mix well, stand for 6 minutes, add 1ml of 10% aluminum nitrate solution (mass percentage concentration), shake well, stand for 6 minutes. Add 10ml of sodium hydroxide test solution, add 30% ethanol to the scale, shake well, let stand for 15 minutes, use the corresponding reagent as a blank, measure the absorbance at a wavelength of 510nm by UV-visible spectrophotometry, ...

Embodiment 3

[0046] Preparation of Alpine Gentian Extract

[0047] 50 g of commercially available Gentiana algida (Gentiana algida Pall.) was dried and pulverized, mixed with 1000 mL of 70% ethanol solution (v / v, all ethanol solutions in the present invention were volume concentrations), soaked, ultrasonically assisted for 1 hour, filtered, After the solvent was removed from the filtrate under reduced pressure, 7.0 g of the crude extract of Gentiana alpine was obtained.

[0048] Get the crude extract of Gentiana alpine, dissolve it with deionized water, load it into the AB-8 resin-packed column, and use deionized water, 30% ethanol, 70% ethanol and 95% ethanol gradient elution successively (percentage is volume percent ), collect eluent respectively, remove ethanol and water under reduced pressure, weigh, obtain water elution part 4.7g, 30% ethanol elution part 0.6g, 70% ethanol elution part 1.0g and 95% ethanol elution part respectively 0.7g of alpine gentian extract.

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Abstract

The invention discloses plant extract composition and an application thereof. The plant extract composition comprises limonium extract and Chinese gentian extract in a mass ratio being (1:99)-(99:1); the limonium extract is prepared with the following preparation method: crude extract of limonium is dissolved in water, loaded to a resin packed column, eluted with water and an ethanol solution sequentially, eluents are collected respectively, and decompression is performed for removing ethanol and water; the Chinese gentian extract is prepared with the following preparation method: crude extract of Chinese gentian is dissolved in water, loaded to a resin packed column, eluted with water and an ethanol solution sequentially, eluents are collected respectively, and decompression is performed for removing ethanol and water. A preparation method of the plant extract composition is simple, and the prepared plant extract composition can be applied as a tyrosinase inhibitor and an antioxidant active agent and preferentially can be further applied in preparation of skin care products or cosmetics.

Description

technical field [0001] The invention relates to the field of plant extraction, in particular to a plant extract composition and its application. Background technique [0002] In 1956, Harman proposed the theory of free radical aging [Harman, D. Aging: A theory based on free radical and radiation chemistry. J. Gerontol. 1956, 11: 289-300.], which believed that excessive free radicals were the cause of biological aging important reason. According to this theory, excess active oxygen free radicals in the body react with unsaturated fatty acids to produce substances such as malondialdehyde, and malondialdehyde will react with proteins on the cell membrane to produce brown pigment, which precipitates on the skin and becomes various stains. Excessive free radicals can also cause cross-linking and denaturation of collagen fibers and elastic fibers in the skin, making them brittle and losing elasticity. When the skin is insufficiently hydrated, it is easy to break the elastic fiber...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61Q19/08
Inventor 方兆华李慧黄晓青
Owner JALA GROUP CORPORATION
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