Quality control product and application of stably-stored lung cancer methylated gene detection kit

A gene detection and methylation technology, which is applied in the field of quality control products of the lung cancer methylation gene detection kit, achieves the effect of good stability, stable performance and low production cost

Active Publication Date: 2018-05-18
GUANGZHOU JINQIRUI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, conventional quality control products are very easy to degrade, and as a quality control product for detecting lung cancer methylation, its accuracy and stability are a big problem that is difficult to solve

Method used

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  • Quality control product and application of stably-stored lung cancer methylated gene detection kit
  • Quality control product and application of stably-stored lung cancer methylated gene detection kit
  • Quality control product and application of stably-stored lung cancer methylated gene detection kit

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Embodiment 1

[0027] An embodiment of the DNA preservation solution of the present invention, comprising aurintriformyl ammonium salt, glycine and bestatin hydrochloride; The ratio is 100:50000:0.02.

Embodiment 2

[0029] An embodiment of the DNA preservation solution of the present invention, ammonium aurintriformate, glycine and bestatin hydrochloride; and the mol ratio of the ammonium aurintriformate, glycine and bestatin hydrochloride For 500:150000:0.06.

Embodiment 3

[0031] An embodiment of the DNA preservation solution of the present invention, ammonium aurintriformate, glycine and bestatin hydrochloride; and the mol ratio of the ammonium aurintriformate, glycine and bestatin hydrochloride For 300:80000:0.04.

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Abstract

The invention discloses a quality control product of a stably-stored lung cancer methylated gene detection kit. The quality control product comprises a positive reference product, a negative referenceproduct and a detection limit reference product which each comprise corresponding DNA and a corresponding DNA storage solution. The DNA storage solutions can effectively prevent the degradation of free DNA, provide a stable environment for the DNA, and prolong the preservation time of the quality control product; the DNA quality control product containing the DNA storage solutions has the advantages of being low in preparing cost, stable in performance and easy to store; the operations of personnel, the states of instruments, the effectiveness of the kit and the like can be effectively monitored, and the accuracy and reliability of the detection kit are greatly improved.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and in particular relates to a quality control product and application of a stably preserved lung cancer methylation gene detection kit. Background technique [0002] In recent years, DNA methylation markers have played an increasingly important role in the early diagnosis of lung cancer, laying the foundation for the development of non-invasive methods to study specific early markers of lung cancer. Lung cancer cells can release DNA into the blood circulation system, and abnormally high concentrations of circulating DNA can be detected in the serum and plasma of cancer patients. Through comparative studies, it was found that DNA methylation in the promoter region had similar change patterns in lung cancer tissues and corresponding serum and plasma samples, which indicated that the epigenetic changes of related genes in blood samples could be used as markers for early screening of tumors. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6806C12Q1/6886
CPCC12Q1/6806C12Q1/6886C12Q2600/154C12Q2600/166C12Q2527/125C12Q2523/125
Inventor 陈嘉昌张瑶柳俊蒋圆玲陈肖燕
Owner GUANGZHOU JINQIRUI BIOTECHNOLOGY CO LTD
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