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Liquid chromatography detection method for inosinic acid content in chicken

A detection method, liquid chromatography technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of large differences in standard deviation and recovery rate, achieve rapid determination, rapid inosinic acid content, improve precision and The effect of accuracy

Inactive Publication Date: 2018-06-19
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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Problems solved by technology

However, the relative standard deviation and recovery rate of this assay method vary greatly among the reported experimental studies, reflecting that the method may have certain defects

Method used

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  • Liquid chromatography detection method for inosinic acid content in chicken
  • Liquid chromatography detection method for inosinic acid content in chicken
  • Liquid chromatography detection method for inosinic acid content in chicken

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Embodiment Construction

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] see Figure 1-4 , the present invention provides a technical solution: a liquid chromatography detection method for inosinic acid content in chicken, comprising the following steps: the first step, selecting raw materials;

[0026] The second step is to select reagents and main instruments;

[0027] The third step is to prepare the main solution;

[0028] The fourth step, raw material processing;

[0029] The fifth step, chromatographic condition set...

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Abstract

The invention discloses a liquid chromatography detection method for inosinic acid content in chicken. The method comprises the following steps: 1, selecting raw materials; 2, selecting reagents and main instruments; 3, preparing a main solution; 4, treating the raw materials; 5, setting chromatographic conditions; and 6, analyzing and calculating detection results. According to the method disclosed by the invention, ammonium formate adopted in a mobile phase is a water-soluble weak acid salt, and chemical characteristics of the ammonium formate are possibly favorable for prolonging the service life of a chromatographic column, so that the method is applicable to determination of lots of samples. The ammonium formate buffer solution serves as the mobile phase for performing reversed-phaseliquid chromatography, the chromatographic conditions are optimized, the determination precision and accuracy of inosinic acid content in muscle tissue samples are increased, and the aim of accuratelyand rapidly determining the inosinic acid content is achieved. The liquid chromatography detection method can be applied to detection and analysis of the inosinic acid content in fresh meat foods.

Description

technical field [0001] The invention relates to the technical field of liquid chromatography detection, in particular to a liquid chromatography detection method for inosinic acid content in chicken. Background technique [0002] Inosinic acid (IMP), namely inosinic acid, is an important flavor substance in animal tissues, which is closely related to the production of umami taste in livestock and poultry meat. At present, the content of inosinic acid in muscle tissue is regarded as an important indicator of meat quality assessment, and has been widely used in the quality assessment of various animal meat products. The level of its content can indirectly reflect the quality of meat food flavor. For this reason, the research on the determination method of inosinic acid content in muscle tissue has become particularly important. At present, the methods for determining the content of inosinic acid in muscle tissue include thin-layer chromatography, ultraviolet spectrophotometry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 席斌高雅琴褚敏李维红郭天芬陈效威杨晓玲
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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