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Normal-temperature storing liquid for intestinal flora sample as well as preparation method and application of normal-temperature storing liquid

A technology of normal temperature preservation and intestinal flora, applied in biochemical equipment and methods, microorganism-based methods, preservation of microorganisms, etc., can solve the problems of difficult to achieve freezing conditions and inability to guarantee accuracy, and achieve large market prospects and Economic value, the effect of convenient transportation at room temperature

Inactive Publication Date: 2018-06-22
深圳谱元科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biggest defect of this preservation method is that the required cryopreservation conditions are difficult to achieve in many cases
If the subjects take samples by themselves, the structure and abundance of the bacterial flora in the samples may have changed greatly due to storage conditions, logistics environment and other factors during the period after the sample collection is completed and the samples are frozen, and the samples are then used for testing experiments Accuracy can no longer be guaranteed

Method used

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  • Normal-temperature storing liquid for intestinal flora sample as well as preparation method and application of normal-temperature storing liquid
  • Normal-temperature storing liquid for intestinal flora sample as well as preparation method and application of normal-temperature storing liquid
  • Normal-temperature storing liquid for intestinal flora sample as well as preparation method and application of normal-temperature storing liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 This example discloses a normal-temperature preservation solution for intestinal flora samples, which includes the following components: Casson preservative, disodium edetate, Tris-HCl buffer solution, lithium chloride , ethanol and sterile water; the Kathon preservative accounts for 0.5‰ of the total mass fraction of the preservation solution; the molar fraction of the disodium edetate is 5mM; the Tris-HCl buffer The molar volume fraction is 15mM; the molar volume fraction of the lithium chloride is 2mol / L; the volume fraction of the ethanol is 95%; the volume fraction of the sterile water is 2%.

[0021] The preparation process of the preservation solution is as follows:

[0022] Weigh the corresponding mass parts of Casson preservative, and the corresponding mole fraction of disodium edetate and lithium chloride of Tris-HCl buffer solution, dissolve them in the corresponding volume of ethanol, mix well, and then add sterile water , stored at room temperatu...

Embodiment 2

[0026] Example 2 This example discloses a normal-temperature preservation solution for intestinal flora samples, which includes the following components: Casson preservative, disodium edetate, Tris-HCl buffer solution, lithium chloride , ethanol and sterile water; the Kathon preservative accounts for 0.2‰ of the total mass fraction of the preservation solution; the molar fraction of the disodium edetate is 10mM; the Tris-HCl buffer The molar volume fraction is 5mM; the molar volume fraction of the lithium chloride is 2mol / L; the volume fraction of the ethanol is 95%; the volume fraction of the sterile water is 3%.

[0027] Preferably, the preparation process of the preservation solution is as follows:

[0028] Weigh the corresponding mass parts of Casson preservative, and the corresponding mole fraction of disodium edetate and lithium chloride of Tris-HCl buffer solution, dissolve them in the corresponding volume of ethanol, mix well, and then add sterile water , stored at ro...

Embodiment 3

[0033] Example 3 This example discloses a normal-temperature preservation solution for intestinal flora samples, which includes the following components: Casson preservative, disodium edetate, Tris-HCl buffer solution, lithium chloride , ethanol and sterile water; the Kathon preservative accounts for 1‰ of the total mass fraction of the preservation solution; the molar fraction of disodium edetate is 1mM; the Tris-HCl buffer The molar volume fraction is 5 mM; the molar volume fraction of the lithium chloride is 2mol / L; the volume fraction of the ethanol is 95%; the volume fraction of the sterile water is 0.1%.

[0034] The preparation process of the preservation solution is as follows:

[0035] Weigh the corresponding mass parts of Casson preservative, and the corresponding mole fraction of disodium edetate and lithium chloride of Tris-HCl buffer solution, dissolve them in the corresponding volume of ethanol, mix well, and then add sterile water , stored at room temperature t...

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Abstract

The invention belongs to the field of molecular biologic intestinal flora, and specifically relates to normal-temperature storing liquid for an intestinal flora sample as well as a preparation methodand application of the normal-temperature storing liquid. The normal-temperature storing liquid comprises the following components: 0.02-0.1 % by mass of Kathon preservative, 1-10 mM by mole of ethylene diamine tetraacetic acid, 5-20 mM by mole of Tris-HCl buffering solution, 2mol / L by mole of lithium chloride, 95% by volume of ethyl alcohol, and 0-3% by volume of sterile water. The storing liquidis applicable to storing and conveying of intestinal flora sample under normal temperature; the sampled intestinal flora sample is soaked in the storing liquid; the stability of the microorganism structure in the sample can be maintained under normal temperature. Therefore, the sample collecting and storing of a subject can be performed in any time; meanwhile, the sample is conveniently conveyedin normal temperature.

Description

technical field [0001] The invention belongs to the field of intestinal flora of molecular biology, and in particular relates to a normal-temperature preservation solution of intestinal flora samples and its preparation method and application. Background technique [0002] Gut flora, that is, the general term for human intestinal microorganisms. The intestinal tract is not only an important place for human digestion and absorption, but also the largest immune organ, which plays an extremely important role in maintaining normal immune defense functions. The human intestinal tract provides a good habitat for microorganisms. The number of microorganisms in the adult intestinal tract is as high as 10^14, which is close to 10 times the number of human somatic cells; the mass reaches 1.2kg, which is close to the mass of human liver; the number of genes contained in it is about It is 100 times that of the human body itself, and has metabolic functions that the human body itself do...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12R1/01
CPCC12N1/04
Inventor 覃俊杰杨仁涛付志聪
Owner 深圳谱元科技有限公司
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