Single-carrier polycyclic aromatic hydrocarbon mixed bacterial agent with low temperature degradation-resistant effect and preparation method and application thereof

A technology of mixing bacterial agents and polycyclic aromatic hydrocarbons, which is applied in the direction of biochemical equipment and methods, fixed on/in organic carriers, and methods based on microorganisms, which can solve the problem of large differences between application results and experimental results, and unreasonable Remediation of contaminated soil and other issues, to achieve the effect of uniform internal structure, low cost and high efficiency

Active Publication Date: 2018-06-29
宁夏荟峰农副产品有限公司
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  • Claims
  • Application Information

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Problems solved by technology

Some studies have shown that the free microorganisms added to the remediation site may be due to the vicious competition of indigenous bacteria or the difficulty in adapting to the environment, resulting in a large difference between the application results and the experimental results, making it unsuitable for in-situ remediation of contaminated soil.

Method used

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  • Single-carrier polycyclic aromatic hydrocarbon mixed bacterial agent with low temperature degradation-resistant effect and preparation method and application thereof
  • Single-carrier polycyclic aromatic hydrocarbon mixed bacterial agent with low temperature degradation-resistant effect and preparation method and application thereof
  • Single-carrier polycyclic aromatic hydrocarbon mixed bacterial agent with low temperature degradation-resistant effect and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0035] Embodiment A method for degrading polycyclic aromatic hydrocarbon pollutants in freeze-thaw soil

[0036] (1) Culture of Pseudomonas sp. SDR4

[0037] Complete medium: 5g beef extract, 10g peptone, 10g glucose, 5g yeast powder, 5g NaCl, 20g agar, 1000mL distilled water, pH 7.1-7.2, sterilized at 121°C for 20min.

[0038] Get the Pseudomonas sp. (Pseudomonassp.) SDR4 preserved on the slant culture medium with the preservation number of CGMCC NO.14048, inoculate it in the complete medium at an inoculum size of 5%, and culture it on a shaker at 15° C. for 30 minutes at 120 r / min. ~40h, until the bacterial density is 6×10 8 CFU mL －1 , to get Pseudomonas (Pseudomonas sp.) SDR4 bacteria suspension.

[0039] (2) Cultivation of Mortierella alpine JDR7

[0040] Complete medium: 5g beef extract, 10g peptone, 10g glucose, 5g yeast powder, 5g NaCl, 20g agar, 1000mL distilled water, pH 7.1-7.2, sterilized at 121°C for 20min.

[0041] Get the Mortierella alpine (Mortierella alp...

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Abstract

The invention discloses a single-carrier polycyclic aromatic hydrocarbon mixed bacterial agent with low temperature degradation-resistant effect and a preparation method and application thereof. The single-carrier polycyclic aromatic hydrocarbon mixed bacterial agent is prepared from a mixed bacterial agent and a corn cob which is used as a carrier, wherein the mixed bacterial agent is obtained bymixing pseudomonas SDR4 and mortierella alpine JDR4 which are screened from frozen and thawed soil according to a volume ratio of 1:1. The single-carrier polycyclic aromatic hydrocarbon mixed bacterial agent with low temperature degradation-resistant effect has the advantages that the biological activity is good, the cost is low, the preparation technology is simple, the secondary pollution is avoided, the recycling is not needed, the malicious competition, devouring and poisoning to microorganisms by indigenous flora, bacteriophage and toxic matters can be effectively shielded, and the highefficiency can be stably realized in the complicated environment; the effect of restoring the frozen and thawed soil which is polluted by polycyclic aromatic hydrocarbon in cold areas is obvious.

Description

technical field [0001] The invention relates to a microorganism immobilization technology, in particular to using corncobs as a carrier and adopting an adsorption method to immobilize low-temperature resistant mixed bacteria to degrade polycyclic aromatic hydrocarbons in freeze-thaw soil in cold regions. [0002] The Pseudomonas sp. SDR4 referred to in the present invention has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on April 19, 2017, and the preservation number is CGMCC NO.14048. [0003] Mortierella alpine (Mortierella alpine) JDR7 referred to in the present invention has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on January 5, 2018, and the preservation number is CGMCC NO.15183. Background technique [0004] Polycyclic aromatic hydrocarbons (Polycyclic aromatic hydrocarbons, PAHs) are a class of organic compounds composed of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02B09C1/10A62D3/02C12R1/38C12R1/645A62D101/20
CPCA62D3/02A62D2101/20B09C1/10B09C1/105C12N11/02
Inventor 苏丹巩春娟陈忠林普聿
Owner 宁夏荟峰农副产品有限公司
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