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Method for detecting symbolic components in soft capsules

A detection method and soft capsule technology are applied in the field of high-performance liquid phase determination of seven iconic components in ginseng and propolis soft capsules, which can solve the problems of easy occurrence of false positives, poor specificity, and inability to accurately and effectively control product quality.

Inactive Publication Date: 2018-06-29
INNER MONGOLIA QITE JINSHENG BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Since the colorimetric method is not chromatographically separated and has poor specificity, if the total saponins and total flavonoids content of ginseng propolis soft capsules or similar products are determined by ultraviolet spectrophotometry, false positives are likely to occur, and product quality cannot be accurately and effectively controlled

Method used

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  • Method for detecting symbolic components in soft capsules
  • Method for detecting symbolic components in soft capsules
  • Method for detecting symbolic components in soft capsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 specificity

[0022] Chromatographic conditions

[0023] Agilent ZORBAX Eclipse XDB-C18 (4.6mm×250mm; 5μm, carbon loading 10%); mobile phase A is 0.15% phosphoric acid solution; mobile phase B is acetonitrile; gradient elution conditions are 0-35min, 19% B; 35 -55min, 19%B→29%B; 55-70min, 29%B; 70-80min, 29%B→37%B; 80-110min, 37%B; volume flow 1.0ml / min, ginsenoside Re, Ginsenoside Rg 1 , Ginsenoside Rb 1 The detection wavelength is 203nm, the detection wavelength of chrysin and galangin is 270nm, the detection wavelength of chopinenin is 289nm, and the detection wavelength of phenethyl caffeate is 329nm.

[0024] Preparation of the test solution: Take 10 ginseng propolis soft capsules, cut them open, mix the contents evenly, accurately weigh 1.5g and place it in a stoppered conical flask, add 50ml of ethanol accurately, weigh, and take it out by ultrasonication for 40min. Let it cool, make up the weight with ethanol, shake well, filter, and take the filtr...

Embodiment 2

[0028] Example 2 Linear

[0029] Precisely draw 0.5, 1.0, 2.0, 2.5, 3.0, 4.0ml of the mixed reference stock solution prepared by the method under "Example 1", place them in a 5mL measuring bottle respectively, dilute to volume with ethanol, shake well, and mix with the stock solution Together as a series of mass concentrations of the mixed reference solution. Draw 10 μL each of the above-mentioned series of mixed reference substance solutions, inject them into the liquid chromatograph, and record the chromatograms. Carry out linear regression with peak area (Y) to concentration (X), get regression equation,

[0030] Ginsenoside Rg 1 : Y=5991.7X+111244, R 2 =0.9993, the linear range is 14.12μg / ml~141.20μg / ml;

[0031] Ginsenoside Re: Y=10125X+12378, R 2 =0.9992, linear range 13.92μg / ml~139.20μg / ml;

[0032] Ginsenoside Rb 1 : Y=3366.2X+45705, R 2 =0.9996, linear range 14.39μg / ml~143.90μg / ml;

[0033] Chrysin: Y=50068X+491441, R 2 =0.9994, linear range 20.42μg / ml~204.20μ...

Embodiment 3

[0037] Embodiment 3 instrument precision

[0038] Precisely absorb the mixed reference substance solution with intermediate mass concentration under the linear relationship investigation item, inject 6 consecutive samples, inject liquid chromatography according to the above chromatographic conditions, record the chromatogram, and measure the peak area value of each substance to be tested. Results The RSDs (n=6) of the peak areas of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, chrysin, galangin, caffeic acid phenethyl ester were 0.30%, 0.31%, 0.33%, 0.22%, respectively. 0.29%, 0.37%, 0.38%, indicating that the precision of the instrument is good.

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Abstract

The invention relates to a method for determining seven symbolic components in ginseng and propolis soft capsules by means of high performance liquid chromatography (HPLC). According to the method, octadecylsilane-bonded silica gel is used as a filler; a mobile phase A is 0.15% phosphoric acid solution; a mobile phase B is acetonitrile; the gradient elution conditions are as follows: 0-35 min, 19%B; 35-55 min, 19% B-29% B; 55-70 min, 29% B; 70-80 min, 29% B-37% B; 80-110 min, 37% B; the volume flow is 1.0 ml / min; the detection wavelengths of ginsenoside Re, ginsenoside Rg1 and ginsenoside Rb1are 203 nm, the detection wavelengths of chrysin and galangin are 270 nm, the detection wavelength of pinocembrin is 289 nm, and the detection wavelength of phenethyl caffeate is 329 nm. The method is simple to operate, accurate in measurement and high in specificity.

Description

technical field [0001] The invention relates to a high-efficiency liquid phase analysis method, in particular to a high-efficiency liquid phase determination method for seven symbolic components in ginseng propolis soft capsules. Background technique [0002] Ginseng Propolis Soft Capsule is a soft capsule made of ginseng extract and wine-made propolis powder as the main raw materials, and is used to assist in lowering blood sugar. Among them, the symbolic components in ginseng are ginsenosides, and the symbolic components in propolis are flavonoids. [0003] The determination methods of total saponins reported in the literature include: vanillin colorimetric method, thin-layer chromatography, GC method, HPLC method, LC-MS method, CE method, etc. The determination method of total saponins in health food provided by Health Food Evaluation and Technical Specification (2003 Edition) is a classic method for the determination of total saponins in health food at present. However...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 佘宜寰林洋上官同强韩风雨于秀玲
Owner INNER MONGOLIA QITE JINSHENG BIOTECH CO LTD
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