LC (liquid chromatography) for concentration determination of clonazepam in blood

A technology for clonazepam and blood is applied in the field of detecting the concentration of clonazepam in blood, which can solve the problems of inaccurate qualitative and quantitative, low sensitivity, many interference factors, etc., and achieves good reproducibility and accurate quantitative analysis. , good specificity

Inactive Publication Date: 2018-07-20
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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Problems solved by technology

The previous methods have the problems of complicated pre-processing, many interference factors, long analysis time, low sensitivity, and inaccurate qualitative and quantitative analysis.

Method used

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  • LC (liquid chromatography) for concentration determination of clonazepam in blood
  • LC (liquid chromatography) for concentration determination of clonazepam in blood
  • LC (liquid chromatography) for concentration determination of clonazepam in blood

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Embodiment Construction

[0037] In this embodiment, the technical method is demonstrated as follows:

[0038] A method for detecting clonazepam drug concentration in blood of the present invention, wherein: it comprises the following steps:

[0039] (1) Calibration of standard solution

[0040] First, mix 10 μL of standard working solution of seven different concentrations with 10 μL of internal standard working solution and 80 μL of complex solution composed of 1:1 methanol and water to make seven standard solutions. After spinning and mixing 3min, use liquid phase chromatographic analysis instrument and ultraviolet detector to detect above-mentioned standard solution, draw the clonazepam of above-mentioned seven kinds of standard solutions and the internal standard chromatogram, in above-mentioned clonazepam and internal standard The peak area of ​​the standard target substance and the peak area of ​​the internal standard substance were obtained respectively in the chromatogram, and the ratio of th...

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Abstract

The invention provides a method for concentration determining concentration of clonazepam in blood. The method comprises the following steps: a liquid chromatograph and an ultraviolet detector are used for calibrating a standard solution, fitting is performed, a standard curve equation of y = a*x+b is obtained, a to-be-detected blood sample is taken, treated and then detected by the liquid chromatograph and the ultraviolet detector, y of the to-be-detected blood sample is obtained and substituted into the standard curve equation, relative concentration x of a target in the to-be-detected bloodsample is obtained by calculation, the concentration of internal standard working fluid is known, and accordingly, the concentration of clonazepam in the to-be-detected blood sample is calculated. The method has the advantages of being rapid, high in extraction rate, good in reproducibility and specificity, high in separation efficiency and accurate in quantitative analysis, can meet the monitoring requirements of plasma concentration of the anti-epilepsy drug clonazepam, and provides a basis for individualized administration of clinical clonazepam.

Description

technical field [0001] The invention relates to the technical field of clinical blood drug concentration monitoring of psychotropic drugs, in particular to a method for detecting the concentration of clonazepam in blood. Background technique [0002] Clonazepam (CZP) is a broad-spectrum anticonvulsant that can also be used for anxiety neurosis and is a high-order benzodiazepine. Its molecular formula is C 15 h 10 ClN 3 o 3 , the chemical structure is 1,3-dihydro-7-nitro-5-(2-chlorophenyl)-2H-1,4-benzodiazepine-2-one, the specific structural formula is as follows figure 1 shown. Clonazepam is clinically used to treat neurological diseases, and its therapeutic window is narrow, with an effective blood concentration of only 20-70 ng / mL; and the pharmacokinetic characteristics vary greatly among individuals, so individualized drug administration is advocated clinically. Adverse reactions are mostly symptoms of the central nervous system, and behavioral abnormalities may al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 贾永娟张杰邱天祎倪君君郑春梅
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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