Animal echinococcosis granulosa ELISA antibody detection kit

A technology for echinococcosis and antibody detection, which is applied in the field of genetic engineering technology and diagnostic reagents, can solve problems such as instability, complex components, and limited sources, and achieve good stability, high sensitivity, and strong specificity of the kit Effect

Inactive Publication Date: 2018-07-27
QINGHAI ACAD OF ANIMAL SCI & VETERINARY MEDICINE
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Currently, hydatid cyst fluid is commonly used in the serological diagnosis of echinococcosis. Due to its complex composition, it has serious cross-reacti

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Preparation of an animal echinococcosis ELISA antibody detection kit

[0017] An animal ELISA antibody detection kit for echinococcosis granulosus, comprising EgAgB8 / 2 recombinant antigen pre-coated microtiter plate, sample diluent, positive control solution, negative control solution, enzyme conjugate, washing solution, substrate display color solution and stop solution.

[0018] The preparation method of the animal echinococcosis ELISA antibody detection kit is as follows:

[0019] 1. The preparation of EgAgB8 / 2 recombinant antigen: at first amplify EgAgB8 / 2 gene cDNA with RT-PCR, clone EgAgB8 / 2 gene, construct prokaryotic expression vector pET-EgAgB8 / 2, and express in Escherichia coli BL21 (DE3), IPTG was used to induce the expression of the prokaryotic expression vector, and the bacteria were collected by centrifugation, dissolved in bacterial lysate and then ultrasonically broken, and the supernatant was collected by centrifugation, and purified by Ni-N...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an echinococcosis granulosa ELISA antibody detection kit. The detection kit comprise an EgAgB8/2 recombinant antigen pre-coating enzyme label plate, a sample weak solution, positive contrast liquid, negative contrast liquid, an enzyme conjugate, washing liquid, a substrate coloring solution and a stopping solution. A method for preparing the EgAgB8/2 recombinant antigen comprises the following steps: using RT-PCR for amplifying EgAgB8/2 gene cDNA, cloning an EgAgB8/2 gene, constructing a prokaryotic expression vector pET-EgAgB8/2, and performing expression in escherichia coli BL21(DE3), using the IPTG-induced prokaryotic expression vector for expression, performing centrifugation to collect thalline, using a bacteria lysate for dissolving, and performing ultrasonicfragmentation, performing centrifugal collection of a supernatant, and using a Ni-NTA glue for purification. The kit takes recombinant protein EgAgB8/2 as antigen to detect an animal echinococcosis granulosa antibody, can be used for qualitative detection of the animal echinococcosis granulosa antibody, and has the advantages of stable antigen source, good specificity, high sensitivity, and good repeatability.

Description

technical field [0001] The invention belongs to the field of genetic engineering technology and diagnostic reagents, in particular to an ELISA antibody detection kit for animal echinococcosis granulosus. Background technique [0002] Echinococcosis, also known as echinococcosis, is a multiple zoonotic parasitic disease with worldwide distribution. It is the most serious parasitic disease that endangers the development of western my country and people's health. The adults of Echinococcus parasitize in the upper small intestines of carnivorous animals such as dogs, foxes, wolves, etc., and are fixed in the crypts at the base of intestinal villi by means of apical hooks and suckers. The intermediate hosts are artiodactyls such as sheep, cattle, camels, deer, mice, certain rodents, etc., and primates (including humans). Animals such as dogs, foxes and wolves are the main transmitters of echinococcosis. There are 5 kinds of Echinococcus that are prevalent in the world, and 3 of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/558G01N33/535
CPCG01N33/535G01N33/558
Inventor 朵红付永何轶群沈秀英郭志宏彭毛李伟
Owner QINGHAI ACAD OF ANIMAL SCI & VETERINARY MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap