Animal echinococcosis granulosa ELISA antibody detection kit

Abstract

The invention discloses an echinococcosis granulosa ELISA antibody detection kit. The detection kit comprise an EgAgB8 / 2 recombinant antigen pre-coating enzyme label plate, a sample weak solution, positive contrast liquid, negative contrast liquid, an enzyme conjugate, washing liquid, a substrate coloring solution and a stopping solution. A method for preparing the EgAgB8 / 2 recombinant antigen comprises the following steps: using RT-PCR for amplifying EgAgB8 / 2 gene cDNA, cloning an EgAgB8 / 2 gene, constructing a prokaryotic expression vector pET-EgAgB8 / 2, and performing expression in escherichia coli BL21(DE3), using the IPTG-induced prokaryotic expression vector for expression, performing centrifugation to collect thalline, using a bacteria lysate for dissolving, and performing ultrasonicfragmentation, performing centrifugal collection of a supernatant, and using a Ni-NTA glue for purification. The kit takes recombinant protein EgAgB8 / 2 as antigen to detect an animal echinococcosis granulosa antibody, can be used for qualitative detection of the animal echinococcosis granulosa antibody, and has the advantages of stable antigen source, good specificity, high sensitivity, and good repeatability.

Description

technical field [0001] The invention belongs to the field of genetic engineering technology and diagnostic reagents, in particular to an ELISA antibody detection kit for animal echinococcosis granulosus. Background technique [0002] Echinococcosis, also known as echinococcosis, is a multiple zoonotic parasitic disease with worldwide distribution. It is the most serious parasitic disease that endangers the development of western my country and people's health. The adults of Echinococcus parasitize in the upper small intestines of carnivorous animals such as dogs, foxes, wolves, etc., and are fixed in the crypts at the base of intestinal villi by means of apical hooks and suckers. The intermediate hosts are artiodactyls such as sheep, cattle, camels, deer, mice, certain rodents, etc., and primates (including humans). Animals such as dogs, foxes and wolves are the main transmitters of echinococcosis. There are 5 kinds of Echinococcus that are prevalent in the world, and 3 of...

AI Technical Summary

Problems solved by technology

[0003] Currently, hydatid cyst fluid is commonly used in the serological diagnosis of echinococcosis. Due to its complex composition, it has serious cross-reaction and non-specific reaction with other helminth patient sera, so the problem of false positive is difficult to overcome. Not ideal and limited sources, unstable