Microwave extraction method for gastroenteropathy Chinese medicinal formula
An extraction method, a technology for gastrointestinal diseases, which is applied in the field of extraction of traditional Chinese medicine compound preparations, can solve problems such as a large amount of time spent, a decrease in the use rate of traditional Chinese medicine, and changes in drug efficacy, and achieve the effect of shortening the extraction time
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Embodiment 1
[0068] This embodiment provides a method for microwave extraction of traditional Chinese medicine prescriptions for gastrointestinal diseases, comprising the following steps:
[0069] 1) Accurately weigh 12g of Jilin ginseng, 18g of betel nut, 20g of peach kernel, and 10g of black medicine with an electronic analytical balance;
[0070] 2) Put the weighed Chinese medicinal materials into the microwave extractor after cleaning;
[0071] 3) Weigh 800mL of distilled water with an electronic balance, and inject it into the microwave extractor;
[0072] 4) Soak for 30 minutes;
[0073] 5) Set the power of the microwave extractor to 600-1200W, and cook for 30 minutes;
[0074] 6) Accurately weigh 12g Amomum seed with an electronic analytical balance, put it into a microwave extractor, mix and decoct for 10 minutes;
[0075] 7) Take a sample every 8 minutes for a total of 5 times. After the extract is cooled to room temperature, put it in the refrigerator for later use.
Embodiment 2
[0077] In this embodiment, by setting up comparative experiments, the influence of microwave power on the extraction effect of arecoline is analyzed. Specifically: under the condition that the soaking time is 30min and the extraction time is 40min, the power of the microwave extractor in step 5) is respectively set to 600W, 800W, 1000W, and 1200W for extraction, and the active ingredients in the extract are determined after the extraction The concentration of arecoline and the influence of microwave power on the extraction effect of arecoline are as follows: figure 1 shown.
[0078] Depend on figure 1 It can be seen that under the same microwave irradiation time, with the increase of microwave power, the concentration of arecoline extracted first increased and then decreased. When the microwave power was 1000W, the concentration of arecoline extracted reached the maximum value, which was 3.91μg / mL. Therefore, the optimal microwave power for arecoline extraction is 1000W.
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Embodiment 3
[0082] In this embodiment, by setting up comparative experiments, the influence of microwave radiation time under microwave power of 600W on the extraction effect of arecoline is analyzed. Specifically: Under the conditions of soaking time of 30min and microwave power of 600W, a sample was taken every 8 minutes for extraction, and the concentration of the active ingredient arecoline in the extract was measured after the extraction, and the influence of extraction time on the extraction effect of arecoline Such as figure 2 shown.
[0083] The results showed that under the power of 600W, with the increase of microwave irradiation time, the concentration of extracted arecoline also increased continuously. After microwave irradiation for 40 minutes, the concentration of arecoline extracted reached the maximum value of 1.29μg / mL. Under the power of 600W, the liquid medicine starts to boil in about 22 minutes. Before 22 minutes, the arecoline in the cells was not extracted, so t...
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