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Preparation method of phycoerythrin labeled polystyrene microsphere fluorescent probe

A technology of polystyrene microspheres and phycoerythrin, applied in fluorescence/phosphorescence, material analysis through optical means, instruments, etc., can solve the problems of easy quenching and poor stability, and achieve difficult quenching and strong stability , the effect of high sensitivity

Inactive Publication Date: 2018-08-10
湖北新纵科病毒疾病工程技术有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these are mainly loaded on polystyrene microspheres by physical adsorption, which has the problem of poor stability or easy quenching.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for preparing a fluorescent probe for phycoerythrin-labeled polystyrene microspheres, comprising the following steps:

[0027] S1: Preparation of surface carboxylated polystyrene microspheres by two-step seed swelling polymerization;

[0028] S11: Weigh 0.1 g of polystyrene seed microspheres and add them to 20 mL of 0.25 wt % sodium dodecyl sulfate (SDS) aqueous solution to form a seed microsphere dispersion, ultrasonically disperse at 200 W for 20 minutes, and take another 0.1 g of cyclohexyl Add alkanes to 20mL of 0.25wt% sodium dodecyl sulfate (SDS) aqueous solution, ultrasonically disperse for 20-40 minutes, add cyclohexane solution to the seed microsphere dispersion, and swell at room temperature for 4-8 hours to obtain the first swelling;

[0029] S12: Add 0.1g dibenzoyl peroxide, 10g styrene, 10g divinylbenzene, 10g toluene, 0.5g acrylic acid and 80mL0.25wt% sodium dodecyl sulfate (SDS) to the first swelling product After stirring the aqueous solution ...

Embodiment 2

[0034] A method for preparing a fluorescent probe for phycoerythrin-labeled polystyrene microspheres, comprising the following steps:

[0035] S1: Preparation of surface carboxylated polystyrene microspheres by two-step seed swelling polymerization;

[0036] S11: Weigh 0.15 g of polystyrene seed microspheres and add them to 20 mL of 0.25 wt % sodium dodecyl sulfate (SDS) aqueous solution to form a seed microsphere dispersion, ultrasonically disperse at 200 W for 20 minutes, and take another 0.15 g of cyclohexyl Add alkanes to 20mL of 0.25wt% sodium dodecyl sulfate (SDS) aqueous solution, ultrasonically disperse for 20-40 minutes, add cyclohexane solution to the seed microsphere dispersion, and swell at room temperature for 4-8 hours to obtain the first swelling;

[0037] S12: Add 0.5g dibenzoyl peroxide, 50g styrene, 25g divinylbenzene, 25g toluene, 2.5g acrylic acid and 80mL0.25wt% sodium dodecyl sulfate (SDS) to the first swelling product After stirring the aqueous solutio...

Embodiment 3

[0042] A method for preparing a fluorescent probe for phycoerythrin-labeled polystyrene microspheres, comprising the following steps:

[0043] S1: Preparation of surface carboxylated polystyrene microspheres by two-step seed swelling polymerization;

[0044] S11: Weigh 0.08g of polystyrene seed microspheres and add them to 16mL of 0.25wt% sodium dodecyl sulfate (SDS) aqueous solution to form a seed microsphere dispersion, ultrasonically disperse at 200W for 20 minutes, and take another 0.13g of cyclohexyl Add alkanes to 24mL of 0.25wt% sodium dodecyl sulfate (SDS) aqueous solution, ultrasonically disperse for 20-40 minutes, add cyclohexane solution to the seed microsphere dispersion, and swell at room temperature for 4-8 hours to obtain the first swelling;

[0045]S12: Add 0.25g dibenzoyl peroxide, 25g styrene, 18g divinylbenzene, 18g toluene, 1.5g acrylic acid and 80mL0.25wt% sodium dodecyl sulfate (SDS) to the first swelling product After stirring the aqueous solution for ...

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PUM

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Abstract

The invention relates to a preparation method of a phycoerythrin labeled polystyrene microsphere fluorescent probe. The method includes: conducting swelling treatment on seed microspheres by two-stepswelling to obtain surface carboxylated polystyrene microspheres, and coupling the carboxyl on the surfaces of the microspheres with phycoerythrin to obtain the phycoerythrin labeled polystyrene microsphere fluorescent probe. The polystyrene microsphere fluorescent probe prepared by the method provided by the invention has the advantages of strong stability, high sensitivity, difficult quenching,high safety factor, and no toxic or side effect.

Description

technical field [0001] The invention relates to a preparation method of a fluorescent-labeled polystyrene probe, in particular to a preparation method of a phycoerythrin-labeled polystyrene microsphere fluorescent probe. Background technique [0002] With the continuous development of materials science, interdisciplinary research between various disciplines. More and more informational and digital information materials are being researched and applied continuously. Recently, the emergence of fluorescent nanoprobes such as inorganic light-emitting quantum dots, fluorescent polymer nanospheres, and composite fluorescent silica nanoparticles, combined with effective physical detection methods, endow sufficient information and provide new developments for biological analysis. field has become a research hotspot in recent years. [0003] Polystyrene is also a fluorescent polymer microsphere that is widely studied and used because of its good mechanical properties and stability....

Claims

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Application Information

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IPC IPC(8): C08F212/08C08F212/36C08F220/06C08G81/02G01N21/64
CPCC08F212/08C08F212/36C08G81/02G01N21/6486C08F220/06
Inventor 王华林张涛张科登
Owner 湖北新纵科病毒疾病工程技术有限公司
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