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Perch interleukin il-12p40 gene and its application

A technology of interleukin and perch, applied in the field of genetic engineering, can solve the problems of less research and incompleteness, and achieve the effect of reducing the mortality rate

Active Publication Date: 2021-04-30
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in general, the research on IL-12p40 in fish, especially perch, is less and incomplete

Method used

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  • Perch interleukin il-12p40 gene and its application
  • Perch interleukin il-12p40 gene and its application
  • Perch interleukin il-12p40 gene and its application

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Embodiment Construction

[0042] The present invention will be further elaborated below through the following specific embodiments, but the content of the present invention is not limited thereto at all.

[0043] 1. Extraction of total RNA and construction of full-length cDNA library

[0044] 1.1 Extraction of total RNA

[0045] Fresh and healthy perch (about 300g in weight) was temporarily raised for 4 days in the Zhuhai Experimental Base of the South China Sea Fisheries Research Institute (the water temperature was about 26±2°C, the air pump was inflated, and the salinity: 10), and the shrimp body was dissected to take out about 500mg of each tissue. Immediately placed in liquid nitrogen, total RNA was purified from dissected tissue (about 50 mg). After low-temperature grinding and crushing, total RNA samples were extracted according to Trizol reagent, and the genome was removed by DNase digestion.

[0046] 1.2 Extraction of total RNA, preparation of full-length cDNA template

[0047] According to...

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Abstract

The present invention discloses a cDNA of interleukin IL-12p40 gene, the nucleic acid sequence of which is shown in SEQ ID NO.1. The coded protein of the perch interleukin IL-12p40 gene is also disclosed, its amino acid sequence is shown in SEQ ID NO.2, and the present invention further discloses an expression vector comprising the cDNA. As well as the application of the coding protein or recombinant protein of the perch interleukin IL-12p40 gene in the preparation of medicines with antibacterial and fish immunity enhancing effects.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the perch interleukin IL-12p40 gene and its application. Background technique [0002] Sea bass can only rely on innate immunity as the first line of defense against invading pathogenic microorganisms and environmental toxic stress. Cytokines play an important role in nearly all aspects of pro-inflammatory and immunity. Interleukin (interleukin, IL) is a class of cytokines with complex immune regulation functions, they are involved in the regulation of cell proliferation, maturation, migration and adhesion, as well as the differentiation and activation of immune cells. Interleukins have pro-inflammatory and anti-inflammatory functions. Some members can act as chemoattractants for T cells, which function similar to chemokines. Others will participate in the antiviral cell response process and have functions similar to interferon . [0003] Interleukin-12...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/24C12N15/70C07K14/54A61K38/20A61P31/04A61P37/04
CPCA61K38/00A61P31/04A61P37/04C07K14/5434
Inventor 邱丽华王鹏飞赵超闫路路范嗣刚陈祥
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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