Lateolabrax japonicus interleukin IL-12p40 gene and application thereof

A technology of interleukin and perch, applied in the field of genetic engineering, can solve the problems of less research and incompleteness, and achieve the effect of reducing the mortality rate

Active Publication Date: 2018-08-21
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in general, the research on IL-12p40 in fish, especially perch, is less and incomplete

Method used

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  • Lateolabrax japonicus interleukin IL-12p40 gene and application thereof
  • Lateolabrax japonicus interleukin IL-12p40 gene and application thereof
  • Lateolabrax japonicus interleukin IL-12p40 gene and application thereof

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Embodiment Construction

[0042] The present invention will be further elaborated below through the following specific embodiments, but the content of the present invention is not limited to this at all.

[0043] 1. Extraction of total RNA and construction of full-length cDNA library

[0044] 1.1 Extraction of total RNA

[0045] Fresh and healthy seabass (weight about 300g) was temporarily cultured in the Zhuhai Experimental Base of the South China Sea Fisheries Research Institute for 4 days (water temperature about 26±2℃, air pump inflation, salinity: 10), the shrimp body was dissected and each tissue was taken out about 500mg, Put it in liquid nitrogen immediately, and purify total RNA from the dissected tissue (about 50 mg). Cryogenic grinding and crushing were carried out, the total RNA sample was extracted according to Trizol reagent, and the genome was digested with DNase.

[0046] 1.2 Extraction of total RNA, preparation of full-length cDNA template

[0047] According to the instructions of PrimeScript™...

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Abstract

The invention discloses a lateolabrax japonicus interleukin IL-12p40 gene and the application thereof. The lateolabrax japonicus interleukin IL-12p40 gene has the nucleic acid sequence shown as SEQ IDNO. The invention further discloses encoded protein of the lateolabrax japonicus interleukin IL-12p40 gene. The encoded protein has the amino acid sequence shown as SEQ ID NO.2. The invention furtherdiscloses an expression vector comprising cDNA and the application of the encoded protein or recombinant protein of the lateolabrax japonicus interleukin IL-12p40 gene to preparation of medicines with antibacterial and fish immunity enhancing efficacies.

Description

Technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to the sea bass interleukin IL-12p40 gene and its use. Background technique [0002] Sea bass can only rely on innate immunity as the first line of defense to prevent the invasion of pathogenic microorganisms and environmental toxic stress. Cytokines play an important role in almost all aspects of pro-inflammatory and immune. Interleukin (IL) is a class of cytokines with complex immune regulation functions. They are involved in regulating cell proliferation, maturation, migration and adhesion, as well as the differentiation and activation of immune cells. Interleukins have pro-inflammatory and anti-inflammatory functions. Some members can act as chemotactic agents for T cells, functioning similar to chemokines, and others will participate in the antiviral cell response process and have functions similar to interferon . [0003] Interleukin 12 is a type of cytokine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/24C12N15/70C07K14/54A61K38/20A61P31/04A61P37/04
CPCA61K38/00A61P31/04A61P37/04C07K14/5434
Inventor 邱丽华王鹏飞赵超闫路路范嗣刚陈祥
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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