Composition for prevention and treatment of bone disease containing mixed Chinese herbal medicine extract

A composition and extract technology, which can be applied in the direction of drug combination, food ingredients containing natural extracts, medical preparations containing active ingredients, etc., can solve the problem of inability to present the therapeutic effect of osteoporosis

Active Publication Date: 2021-07-16
金秀昡
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mixed composition disclosed in the above-mentioned documents cannot exhibit a significant therapeutic effect on osteoporosis. Therefore, there is an urgent need to develop a composition with high therapeutic effect on osteoporosis using natural products that is non-toxic to the human body.

Method used

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  • Composition for prevention and treatment of bone disease containing mixed Chinese herbal medicine extract
  • Composition for prevention and treatment of bone disease containing mixed Chinese herbal medicine extract
  • Composition for prevention and treatment of bone disease containing mixed Chinese herbal medicine extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1. Preparation of mixed crude medicinal material extract

[0057] 1-1. Raw medicinal material screening and pretreatment process

[0058] For 150g of Chinese Angelica, 150g of Radix Paeoniae Alba, 150g of Atractylodes Rhizome, 150g of Poria, 100g of Gardenia, 100g of Licorice, 100g of Bellflower, 50g of Dried Ginger and 50g of Peppermint, after removing foreign matter, finely cut them into about 5cm. Extracts easily and rinses cleanly with water. The purpose of finely chopping raw herbs is to facilitate their handling and extraction.

[0059] 1-2. Preparation of hot water extract of mixed raw medicinal materials

[0060] The material obtained in the above-mentioned process is put into the extraction device, and extracted with an extraction solvent. As an extraction solvent, pure water was added 5 times as much as the crude drug, and extracted at a temperature of 95° C. for 6 hours with a cooling extraction device. The extract was soaked naturally for 1 to...

Embodiment 2

[0072] Example 2. Activity evaluation of osteoblasts

[0073] 2-1. Cell culture and differentiation induction

[0074] MC3T3-E1 osteoblast cells (MC3T3-E1 osteoblastic cells) derived from raw mice were purchased from American Type Culture Collection (ATCC, American Type Culture Collection). MC3T3-E1 cells were prepared by adding 10% fetal bovine serum (FBS, fetal bovine serum) and 100 U / mL penicillin ( penicillin), 100U / mL of streptomycin (streptomycin) in cell culture medium at 37°C in humidified CO 2 Incubator (5% CO 2 / 95% air (air)). If the cells fill up about 80% of the culture dish, then utilize phosphate buffered saline (PBS, pH7.4) to wash the monolayer of cells, and process 0.25% trypsin (trypsin)-2.65mM EDTA ( EDTA) for subculture, and the medium was changed every 2 days.

[0075] When MC3T3-E1 cells filled up about 90% of the culture dish, in order to induce cell differentiation, β-glycerophosphate (β-glycerophosphate) (Sigma-Aldrich) (Sigma- Aldrich Co.)) and...

Embodiment 3

[0086] Example 3. Activity evaluation of osteoclasts

[0087] 3-1. Cell culture and differentiation induction

[0088] Raw264.7 cells were purchased from the American Type Culture Collection (ATCC, American Type Culture Collection) as macrophages derived from raw mice. Raw264.7 cells were prepared by adding 10% fetal bovine serum, 100units / mL of penicillin, and 100ug / mL of streptomycin in Dulbecco's modified Eagle's medium (DMEM, dulbecco's modified eagle medium, Welgene Company). primed cell culture medium at 37 °C in a humidified CO 2 Incubator (5% CO 2 / 95% air (air)). If the cells fill up about 80% of the culture dish, wash the monolayer of cells with phosphate buffered saline (PBS, pH 7.4), add cell culture medium to remove the cells for subculture, and replace them every 2 days Medium.

[0089] To induce the differentiation of Raw264.7 cells, 50 ng / mL of receptor activator ligand (RANKL) (Sigma-Aldrich Co.) and 10 μM of PD98059 were added to α-minimum essential medi...

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Abstract

The present invention relates to a composition for the prevention and treatment of bone diseases containing mixed Chinese herbal extracts, including Angelica (Angelicagigas), Paeoniae Radix Alba (Paeoniae Radix Alba), Atractylodes macrocephala Koidzumi, Poria cocos (Wolfiporia extensa) and Campanulaceae (bellflower) Danghua (Platycodonggrandiflorum A.DC.) root) extract, which consists of gardenia (Gardeniae Fructus), licorice (Glycyrrhiza uralensis, Licorice), dried ginger (Zingiberis Siccatum Rhizoma) and peppermint (Mentha arvensis var. piperascens) The composition of more than one extract from the group presents safe in vivo, increases bone weight and length, increases bone density, and, in particular, significantly increases osteogenesis in the case of compositions containing Platycodon grandiflorum in optimal content Alkaline phosphatase activity of cells, and significantly inhibit the tartrate-resistant acid phosphatase activity of osteoclasts, so it can be effectively applied to the improvement of various bone diseases including osteoporosis.

Description

technical field [0001] The present invention relates to containing Chinese angelica (Angelicagigas), white peony root (Paeoniae Radix Alba), Atractylodes macrocephala (Atractylodes macrocephala Koidzumi), Poria cocos (Wolfiporia extensa), gardeniae Fructus (Glycyrrhiza uralensis, Licorice), bellflower (bellflower ( Platycodong grandiflorum A.DC.), dried ginger (Zingiberis Siccatum Rhizoma) and peppermint (Mentha arvensis var. piperascens) mixed extract as active ingredients for the prevention or treatment of bone diseases composition. Background technique [0002] Bone tissue that constitutes our body is a dynamic tissue that undergoes continuous resorption and formation in order to maintain bone mass and skeletal homeostasis. Osteoblasts and osteoclasts play a role in breaking down and growing new bone. Osteoclasts, derived from hematopoietic stem cells, remove old bone through a process of destruction or resorption and participate in maintaining body function by releasing ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/71A61K36/65A61K36/744A61K36/9068A61P19/10
CPCA23V2002/00A61K36/076A61K36/232A61K36/284A61K36/346A61K36/484A61K36/534A61K36/65A61K36/71A61K36/744A61K36/9068A23L33/105A61K2300/00A23V2200/306A23V2250/21A23V2250/208A23V2300/14A23V2300/10
Inventor 金秀昡俞东辰俞受玎金昌垠全文姬
Owner 金秀昡
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