Method for extracting procyanidin based on liquid-electric effect assistance

Abstract

The invention discloses a method for extracting procyanidin based on liquid-electric effect assistance. According to the method, pulse discharge is generated in a mixture of grape tissues and a solvent; the grape tissues are crushed by utilizing strong shock wave radiated by a liquid-electric effect; in a discharging process, an applied rapid pulse electric field can be used for effectively improving the permeability of a plant cell membrane to cause perforation damages, and the release of the procyanidin is effectively accelerated; active oxygen components in the discharging process further act on the plant cell membrane to accelerate the exudation of polyphenol substances; separation and purification of the procyanidin is carried out on a grape tissue suspension solution, which is treated through the liquid-electric effect, by adopting a combined membrane separation technology according to the difference of molecular weight of high-polymer procyanidin and oligomeric procyanidin in filtrate. Based on the liquid-electric effect assistance, various acting mechanisms can be used for effectively separating and purifying the procyanidin from the grape tissues and a chemical organic solvent is not needed; the bioactivity of the procyanidin can be effectively kept and the method has the characteristics of greenness and no pollution, simplicity in operation, good economical efficiencyand the like.

Description

technical field [0001] The invention belongs to high-voltage technology, pulse power technology and a process for extracting active ingredients from natural products, and more specifically relates to a method for assisting the extraction of proanthocyanidins from grape extracts based on a hydroelectric pulse method. Background technique [0002] Proanthocyanidins are a class of polyphenols that are formed by connecting monomers such as catechin, epicatechin and epicatechin gallate through carbon bonds, and are widely found in grapes, pine trees and other plants. Among them, various extracts of grapes contain a large amount of proanthocyanidins, among which oligomeric proanthocyanidins are the most efficient antioxidant substances found in plant sources so far, and have very strong in vivo activity, and their anti-free radical oxidation ability is several times that of general vitamins. ten times. However, the domestic extraction and separation of proanthocyanidins in grape ...

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Problems solved by technology

[0004] Aiming at the above defects or improvement needs of the prior art, the present invention provides a method for assisting the extraction of proanthocyanidins based on the hydroelectric effect, thus solving the problems of long extraction time, low extraction efficiency, pollution, and production costs in the existing extraction of proanthocyanidins. High and technical problems that cause loss of active ingredients

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