Flavonoid effective part of flos abelmoschus manihot as well as preparation method and application of flavonoid effective part

A technology of flavonoids and effective parts of marshmallow flowers, which is applied in the field of medicine, can solve problems such as poor efficacy, and achieve the effects of improving compliance, good repeatability, and reducing dosage

Active Publication Date: 2018-09-25
JIANGSU SUZHONG PHARM GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a kind of flavonoid effective part of marshmallow flower and its preparation method and application, so as to solve the problems such as poor effect existing in the prior art

Method used

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  • Flavonoid effective part of flos abelmoschus manihot as well as preparation method and application of flavonoid effective part
  • Flavonoid effective part of flos abelmoschus manihot as well as preparation method and application of flavonoid effective part
  • Flavonoid effective part of flos abelmoschus manihot as well as preparation method and application of flavonoid effective part

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, macroporous resin impurity removal process

[0042] Take HPD-100 macroporous resin, wet-pack the column (4cm*20cm), the bed volume (BV) is 250mL. Load 250 mL of 4.0 mg / mL hollyhock flower extract solution. First elute with 4BV of water, then remove impurities with low-concentration ethanol solution, and finally desorb with 4BV of 70% ethanol solution. The ethanol was recovered from the desorption solution, and a part was taken to measure the solid content, and the content of the flavonoids was determined by the UPLC method reported in "Determination of 7 flavonoids in hollyhock flower", the same below.

[0043] The ethanol concentration of the impurity removal solution, the volume of the impurity removal solution, and the elution flow rate were selected as the investigation factors, and three levels were designed for each factor, and an orthogonal experiment was carried out. The content ratio of the seven flavonoids in the eluent was used as the evaluatio...

Embodiment 2

[0051] Embodiment 2, macroporous resin desorption process

[0052] Take HPD-100 macroporous resin, wet-pack the column (4cm*20cm), the bed volume (BV) is 250mL. Load 250 mL of 4.0 mg / mL hollyhock flower extract solution. First elute with 4BV water, then remove impurities with 30% ethanol solution, and finally desorb with higher ethanol solution. Ethanol was recovered from the desorption liquid, and a part was taken to measure the solid content, and the content of flavonoids was determined by UPLC method.

[0053] The ethanol concentration of the desorption solution, the volume of the desorption solution, and the elution flow rate were selected as the investigation factors, and three levels were designed for each factor, and an orthogonal experiment was carried out, as shown in Table 3.

[0054] table 3

[0055]

[0056] Orthogonal design was used to investigate the influence of ethanol solutions with different concentrations, different dosages and different flow rates on...

Embodiment 3

[0061] Embodiment 3, macroporous resin purification scale-up test

[0062] Take HPD-100 macroporous resin and wet-pack the column (9cm*45cm), with a bed volume (BV) of 2.86L. Load 5.72L of 25mg / mL hollyhock flower extract solution. Purify according to the preferred process conditions, collect the stripped solution, concentrate and dry.

[0063] The content of 7 kinds of flavonoids in it was determined by UPLC method, reaching 51.43%; while the content of 7 kinds of flavonoids in Huangkui dry extract was 8.29%. Using ultraviolet spectrophotometry, using hyperin as the reference substance, the total flavonoid content was determined to reach 70.56%, while the total flavonoid content in the dried extract of Amla japonica was 20.25%.

[0064] It shows that the purification method of flavonoids in ambrette still has a good effect of purifying flavonoids after being enlarged.

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Abstract

The invention discloses a flavonoid effective part of flos abelmoschus manihot as well as a preparation method and application of the flavonoid effective part. Compared with the prior art, the flavonoid effective part of the flos abelmoschus manihot increases the content of kidney protection active ingredients in a flos abelmoschus manihot extract, and reduces the administration dosage of a patient under the premise of having the same effect so as to improve patient compliance.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to an effective fraction of flavonoids from marshmallow flowers, a preparation method and application thereof. Background technique [0002] Hollyhock flower is the dry corolla of Abelmoschus manihot (L.) Medic. The hollyhock flower was first recorded in "Jiayou Materia Medica", and is now included in the 2015 edition of "Chinese Pharmacopoeia". Modern medical research has proved that hollyhock flower has better effects on protecting the kidney, improving kidney function, and treating chronic nephritis. The marshmallow flower extract has been developed and produced by Jiangsu Suzhong Pharmaceutical Group Co., Ltd. to produce Huangkui Capsules. It has the effects of clearing away dampness and heat, detoxifying and reducing swelling. It is mainly used for the treatment of chronic nephritis and has a significant clinical effect. However, Huangkui Capsules has the proble...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P13/12A61K133/00
CPCA61K36/185A61K2236/333A61K2236/39A61K2236/51A61K2236/55A61P13/12
Inventor 赵筱萍唐海涛杨振中葛海涛马继梅
Owner JIANGSU SUZHONG PHARM GRP CO LTD
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