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A preparation method of a blood-contact material for improving biocompatibility and the blood-contact material

A technology of biocompatibility and contact materials, applied in the field of biomedical functional materials, can solve the problems of high incidence of adverse reactions, unstable anticoagulant implantation in vivo, etc., to improve biocompatibility and reduce incidence , Promote the effect of endothelial repair

Active Publication Date: 2020-09-08
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the blood-contact materials immobilized with single HA or releasing NO still face problems such as anticoagulant properties to be further improved, unstable implantation in the body leading to inflammation in the body, etc.
The reason is that the adverse reactions after implantation of blood-contacting materials often occur through multiple pathways, and the surface modification of blood-contacting materials such as HA and other single endothelial cell products or single functional factors such as secreting NO can inhibit some of these pathways, but adverse effects cannot be achieved. Reactions can still occur through other routes, resulting in a relatively high incidence of adverse reactions

Method used

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  • A preparation method of a blood-contact material for improving biocompatibility and the blood-contact material
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  • A preparation method of a blood-contact material for improving biocompatibility and the blood-contact material

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preparation example Construction

[0036] An embodiment of the present invention provides a method for preparing a blood contact material for improving biocompatibility, which includes the following steps:

[0037] S1 preparation activator: 2-(N-morpholine)ethanesulfonic acid (MES), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), N- Mix hydroxysuccinimide (NHS) and water to prepare the activator. Generally, a certain amount of MES solution is first prepared at a concentration of 40-60mmol / L, then a certain amount of EDC and NHS are added, shaken and mixed to prepare an activator, and the concentration of MES in the obtained activator is 40-60mmol / L. The concentration of EDC is 5-7mmol / L, and the concentration of NHS is 8-12mmol / L.

[0038] S2 modified hyaluronic acid: immerse the base material of amino-rich coating in the first mixed solution prepared by hyaluronic acid solution (HA solution) and activator for the first amide reaction, the first amide reaction is generally at 35 The amide r...

Embodiment 1

[0049] The embodiment of the present invention provides a blood contact material, which is modified with HA+SeCA, and it is prepared according to the following preparation method:

[0050] First prepare a certain amount of MES solution at a concentration of 50mmol / L, then add a certain amount of EDC and NHS, shake and mix to prepare an activator, the concentration of MES in the obtained activator is 50mmol / L, and the concentration of EDC is 6mmol / L The concentration of L and NHS is 10mmol / L.

[0051] According to the molar ratio of -COOH in the HA solution to EDC and NHS in the activator is 1:6:10, the material is taken out, the activator is added to the HA solution, and the first mixed solution is obtained after activation for 30 minutes. The substrate material was immersed in the first mixed solution for the first amide reaction at 37° C. for 24 hours, and then taken out and washed with deionized water for 3 times to obtain the HA modified material.

[0052] Prepare a SeCA ...

Embodiment 2

[0054] The embodiment of the present invention provides a blood contact material, which is modified with HA+SeCA, and it is prepared according to the following preparation method:

[0055] First prepare a certain amount of MES solution at a concentration of 40mmol / L, then add a certain amount of EDC and NHS, shake and mix to prepare an activator, the concentration of MES in the obtained activator is 40mmol / L, and the concentration of EDC is 7mmol / L The concentration of L and NHS is 12mmol / L.

[0056] According to the molar ratio of -COOH in the HA solution to EDC and NHS in the activator is 2:7:12, the material is taken out, the activator is added to the HA solution, and the first mixed solution is obtained after activation for 10 minutes. The base material was immersed in the first mixed solution for the first amide reaction at 35° C. for 40 hours, and then taken out and washed with deionized water for 3 times to obtain the HA modified material.

[0057] Prepare a SeCA solut...

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Abstract

The invention relates to a preparation method of blood contact material for improving the biocompatibility and a blood contact material, and relates to the field of biomedical functional materials. The preparation method of the blood contact material for improving the biocompatibility is that the MES, the EDC, the NHS and the water are uniformly mixed and prepared into an activating agent; the base material with the amino-rich coating is immersed in the first mixed solution prepared by the hyaluronic acid solution and the activating agent to carry out the first amine reaction, then is taken out for cleaning with water for a plurality of times to obtain the HA-modified material; the HA-modified material is immersed in a second mixed solution prepared by the selenocysteamine solution and theactivating agent to carry out the second amine reaction, then is taken out for cleaning with water for multiple times, and the blood contact material is obtained. The preparation method can remarkably improve the biocompatibility of the material, so as to reduce the incidence of adverse reaction; the blood contact material has good anti-coagulation, smooth muscle hyperplasia, and endothelial repair function and biocompatibility.

Description

technical field [0001] The invention relates to the field of biomedical functional materials, and in particular to a preparation method of a blood contact material for improving biocompatibility and the blood contact material. Background technique [0002] According to modern human health research, cardiovascular disease has become the number one killer threatening human life and health. Treatment methods based on cardiovascular implants such as cardiovascular stents and artificial blood vessels are currently the main means of dealing with cardiovascular diseases. Cardiovascular implantable devices often face very harsh working environments such as blood coagulation, inflammation, intimal hyperplasia, difficult endothelial repair, and late thrombosis, so very high requirements are placed on the biocompatibility of blood-contact materials. The natural vascular endothelial layer has the most perfect biocompatibility, and has many functions such as resisting coagulation, inhib...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/34A61L27/50A61L33/04A61L33/06
CPCA61L27/34A61L27/50A61L33/0011A61L33/0088A61L2300/204A61L2300/412A61L2300/416A61L2300/418A61L2420/06C08L5/08
Inventor 杨苹谢州陈江廖玉珍黄楠
Owner SOUTHWEST JIAOTONG UNIV
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