30 results about "Endothelial repair" patented technology

The invention relates to an application of amlexanox or salt thereof or a solvate thereof in preparing medicines having a smooth muscle cell inhibiting effect, in particular to an application in medicines for preventing and treating vascular restenosis. The invention also relates to the technical field of medical apparatuses. The amlexanox or the salt or the pharmaceutical composition thereof is distributed on the surface of the medical apparatus, in particular a drug stent. A completely new bio-activity of the amlexanox is discovered, exactly the amlexanox is high in activity of inhibiting smooth muscle cell proliferation; in comparison with currently common smooth muscle cell proliferation inhibitors, the amlexanox also has the characteristic of being low in performance of inhibiting endothelial cell growth; therefore, the amlexanox is especially applicable to medical apparatuses; and the amlexanox can prevent the vascular restenosis occurrence rate, and in addition, the amlexanox can prevent delay of endothelial repair; therefore, the time of taking anti-thrombus and antiplatelet medicines of a patient after an operation can be shortened, and a long-term curative effect can be greatly improved.

The present invention relates to a method for diagnosing and treating arteriosclerosis using a nanovesicle targeted at a blood flow change site, and more particularly, to: a stem cell-derived nanovesicle as an anti-arteriosclerosis diagnosis and treatment platform using a peptide capable of targeting at a blood flow disorder site; the nanovesicles provide strong anti-inflammatory and endothelial repair promoting effects similar to those of mesenchymal stem cells, and thus can be used as a novel diagnostic and therapeutic agent capable of preventing the onset of arteriosclerosis.

The invention belongs to the field of medical devices, and in particular relates to a drug support and a preparation method thereof. The drug stent includes a stent body and a drug composition distributed on the stent body, and the drug composition is composed of amlexanol, paclitaxel, sirolimus and aspirin. The preparation method comprises the following steps: firstly preparing the mixed powder or solution of the pharmaceutical composition, and then loading the prepared mixed powder or mixed solution onto the surface of the stent body by dripping, filling or coating . The present invention finds that the composition of amlexanox, paclitaxel, sirolimus and aspirin has a high activity of inhibiting the proliferation of smooth muscle cells. It also has the characteristics of low endothelial cell growth inhibition, so it can prevent the incidence of vascular restenosis and will not delay endothelial repair during the treatment after PTCA.

The invention belongs to the technical field of genetic engineering, and in particular relates to a fusion protein for promoting endothelial repair and its preparation method and application. The amino acid sequence of the fusion protein promoting endothelial repair of the present invention is shown in SEQ ID NO.1. The fusion protein for promoting endothelial repair provided by the present invention can efficiently and specifically promote the adhesion of endothelial cells and endothelial progenitor cells, can promote the proliferation of endothelial cells, and will not increase the adhesion of smooth muscle cells and endothelial inflammation, and is beneficial to endothelial cells. Cellular tight junctions and NO release, thereby accelerating endothelialization of blood vessels. The fusion protein or its prepared recombinant carrier can be used in the preparation of biomaterials promoting endothelial repair, and has important clinical significance.

The invention relates to a preparation method of blood contact material for improving the biocompatibility and a blood contact material, and relates to the field of biomedical functional materials. The preparation method of the blood contact material for improving the biocompatibility is that the MES, the EDC, the NHS and the water are uniformly mixed and prepared into an activating agent; the base material with the amino-rich coating is immersed in the first mixed solution prepared by the hyaluronic acid solution and the activating agent to carry out the first amine reaction, then is taken out for cleaning with water for a plurality of times to obtain the HA-modified material; the HA-modified material is immersed in a second mixed solution prepared by the selenocysteamine solution and theactivating agent to carry out the second amine reaction, then is taken out for cleaning with water for multiple times, and the blood contact material is obtained. The preparation method can remarkably improve the biocompatibility of the material, so as to reduce the incidence of adverse reaction; the blood contact material has good anti-coagulation, smooth muscle hyperplasia, and endothelial repair function and biocompatibility.

The invention relates to the field of interventional medical devices, and especially relates to a surface modification method for improving the corrosion resistance of a magnesium alloy intravascularstent. The method is suitable for a novel dual-drug degradable magnesium alloy intravascular stent for treating intraluminal stenosis, carrying a smooth muscle proliferation inhibitor and promoting endothelialization. The method is characterized in that a drug coating with an endothelialization promoting function is prepared on a magnesium alloy stent to accelerate the endothelialization process after stent implantation and improve the service environment of the stent, so the corrosion resistance of the magnesium alloy intravascular stent is improved. The magnesium alloy intravascular stent carries a drug for inhibiting intimal hypertrophy, and also carries a drug for promoting endothelialization. The stent can realize rapid endothelial regeneration of damaged parts through the action of the drug for promoting endothelialization after being implanted into a diseased blood vessel, so the service environment of the magnesium alloy stent is improved, and the corrosion resistance of the degradable magnesium alloy intravascular stent is improved.

The invention provides a method for preparing an endothelial progenitor cell capturing bracket for gene modification and regulation, which comprises the following steps: 1, preprocessing a bare bracket; 2, preparing an endothelial progenitor cell antibody bracket; 3, combining Caspase genes and specific promoter gene segments differentiated from smooth muscle cells in a gene recombination way andthen combining the composition of the Caspase genes and the specific promoter gene segments and eukaryotic expression vectors pEGFPC2 or pcDNA3.1 in the gene recombination way to obtain fusion genes;and 4, dissolving the fusion genes obtained in the step 3 in de-ionized water solution to obtain solution of which the concentration of the fusion genes is 10 to 100 mg / ml, placing the endothelial progenitor cell antibody bracket obtained in the step 2 into the solution for co-incubation at 4 DEG C for 12 minutes, taking the endothelial progenitor cell antibody bracket out, naturally airing the endothelial progenitor cell antibody bracket in a super clean bench for 2 to 6 hours and storing the endothelial progenitor cell antibody bracket at 4 DEG C. The method has the advantages of dual functions of resisting restenosis and protecting endothelial repair.

The invention discloses a construction method of an enzyme-reponsive multifunctional nano-coating. The construction method comprises the following steps: firstly, immobilizing avidin molecules to the surface of dopamine-coated stainless steel; then, on the basis of specific recognition and binding effects between avidin and biotin, immobilizing biotinylated heparin / PEI nanoparticles to the surface of a material, and further binding high-concentration avidin molecules to residual biotin on the surface of a nanoparticle coating, so that a novel biotin binding site is introduced; then, continuing to assemble biotinylated enzyme-responsive polypeptide to the surfaces of the nanoparticles on the basis of an interaction between the biotin and the avidin; and finally, by virtue of an EDC / NHS / MES coupling agent, immobilizing SDF-1[alpha] to an amino terminal of the polypeptide in a covalent mode, so that the multifunctional nano-coating, which has a matrix metalloproteinase 9 responsive characteristic, is constructed. According to the construction method provided by the invention, by constructing a multifunctional layer, which has anti-coagulating and endothelial regeneration inducing capacities, on a titanium surface, blood compatibility and a damaged endothelial repair capacity of the material can be obviously improved.

The present invention relates to the field of therapy and prophylaxis of vascular tissue damage and / or complications and / or disease of vascular tissue by vascular regeneration and endothelium repair. The inventors have found that administration of an androgen receptor agonist alleviates one or more adverse vascular diseases and / or vascular complications thereof or adverse effects of androgen deficiency, with implications for prophylactic and therapeutic interventions.