A gold nanocluster particle for responsive blood sugar regulation and preparation method thereof
A technology of gold nanoclusters and particles, applied in the field of biomedicine, can solve the problems of low blood sugar concentration endangering life and health, affecting the quality of life, psychological pressure of patients, etc., and achieving the effect of real-time response to glucose concentration, regulating human blood sugar, and reducing hyperglycemia
Active Publication Date: 2020-06-09
WUHAN UNIV
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Problems solved by technology
Long-term frequent subcutaneous insulin injections bring severe psychological pressure to patients and affect their quality of life
At the same time, if the injection dose cannot be accurately controlled, it is easy to cause hyperglycemia or hypoglycemia, which can induce serious complications, or the low blood glucose concentration in the patient's body will endanger life and health.
Method used
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Embodiment 1
[0035] (1) Preparation of BSA-coated gold nanocluster particles (BSA-GNCs)
[0036] In the fume hood, clean the magnets and round bottom flasks to be used with aqua regia, clean them with water and alcohol, and dry them for use.
[0037] Prepare 25mL of 10mM chloroauric acid (HAuCl 4 ) solution, prepare 25mL 50mg / mL bovine serum albumin (BSA), mix the two by volume ratio 1:1, then HAuCl in the solution 4 The concentration of BSA is 5mM, and the concentration of BSA is 25mg / mL (ie 0.375mM). The mixed solution was heated to 37°C, and a small amount of NaOH solution was added to adjust the pH value to 12. Heated in a constant temperature water bath at 37°C, stirred at a speed of 500 rpm, and reacted in the dark for 12 hours. The solution color changed from bright yellow to light brown, and finally to dark brown, forming BSA-coated gold nanocluster particles (BSA-GNCs) ( figure 1 ). The color of the prepared BSA-GNCs solution is dark brown ( figure 2 ). The prepared BSA-GNCs...
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Abstract
The invention discloses responding type gold nano-cluster particles for blood glucose regulation and a preparation method of the responding type gold nano-cluster particles, and belongs to the field of biomedicine. The responding type gold nano-cluster particles comprise a carrier material, a glucose responding sensitive switch factor and pharmaceutical molecules. The preparation method comprisesfollowing steps: firstly, preparing BSA-coated gold nano-cluster particles BSA-GNCs to serve as the carrier material of the pharmaceutical molecules; removing amino on BSA molecules by glutaraldehyde,converting tail ends of the molecules into carboxyl by glycine, activating carboxyl, and grafting and modifying carboxyl with 4-aminophenylboronic acid to serve as the glucose responding sensitive switch factor; grafting the glucose responding sensitive switch factor with glycosylated insulin to obtain the responding type gold nano-cluster particles for blood glucose regulation. The gold nano-cluster particles can release insulin according to glucose concentration response; functions of responding to glucose concentration in real time, rapidly and intelligently transmitting and releasing insulin and regulating blood glucose of a human body are achieved.
Description
technical field [0001] The invention relates to the field of biomedicine, in particular to a gold nano-cluster particle for responsive blood sugar regulation and a preparation method thereof. Background technique [0002] Diabetes is a group of major chronic metabolic diseases characterized by hyperglycemia. Relative or absolute insufficiency of insulin in the human body leads to hyperglycemia, which is the root cause of diabetes. Diabetes has become the third major factor endangering human health. According to statistics, there were 420 million diabetics in the world in 2016, and this number will increase to 629 million by 2045. [0003] Real-time monitoring of blood glucose concentration in the body and timely injection of insulin are the keys to effective control of diabetes. At present, the vast majority of diabetic patients are treated with insulin, that is, taking insulin by injection or orally to regulate blood sugar. The specific treatment method is to monitor the...
Claims
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IPC IPC(8): A61K38/28A61K47/64A61K47/52A61K47/54A61K47/69A61P3/10
CPCA61K38/28A61K47/52A61K47/54A61K47/643A61K47/6941A61P3/10
Inventor 雷祎凤刘胜张玉洁
Owner WUHAN UNIV