Preparation method and application of nano-selenium-amantadine compound nano-drug carrier
A nano-drug carrier, amantadine technology, which can be used in pharmaceutical formulations, medical preparations without active ingredients, and medical preparations containing active ingredients, etc., can solve the problems of antiviral drug treatment and other problems, and achieve good antiviral activity. Effect
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Embodiment 1
[0057] Example 1 Preparation of nano-selenium-amantadine composite nano-drug carrier drug-loading system
[0058] Take 2mL stock solution as 50mM vitamin C solution and add dropwise to 0.25mL concentration of 0.1M sodium selenite (Na 2 SeO 3 ) solution, add deionized water to make the final volume 25mL. Magnetic stirring was carried out for 2 hours, and the purified nano-selenium solution was obtained after dialysis. For dialysis, a dialysis bag with a molecular weight cut-off of 1000 was selected, and the dialysis was carried out for 24 hours. Take 10 mL of the above-mentioned nano-selenium solution, add 0.8 mL of amantadine solution whose stock solution is 1 μM, and stir magnetically for 1 h. The reacted solution undergoes dialysis, and the dialysis is a dialysis bag with a molecular weight cut-off of 1000. Dialysis is performed for 24 h, centrifuged at 10,000 rpm for 10 min, deionized Washing with water three times, and freeze-drying the sample to obtain a nano-seleniu...
Embodiment 2
[0060] Example 2 Characterization of nano-selenium-amantadine composite nano-drug carrier drug-loading system
[0061] Basic physical properties of nano-selenium-amantadine composite nano-drug carriers
[0062] Amantadine (AM), nano-selenium (SeNPs) and nano-selenium-amantadine composite nano-drug carrier (Se@AM) were distinguished from the color appearance and Tyndall effect.
[0063] Such as figure 1 As shown, panel A is the color comparison of amantadine (AM), nano-selenium (SeNPs) and nano-selenium-amantadine composite nano-drug carrier (Se@AM). AM is clear and transparent, and SeNPs are darker than Se@AM, which may be the result of introducing AM into Se@AM; B and C are the Tyndall effect of Se@AM; it can be seen from the figure that Se@AM is different from SeNPs and AM.
[0064] Detection of particle size of nanoselenium-amantadine composite nano drug carrier by nanometer particle size potentiometer (Nano-ZS) and potential
[0065] Such as figure 2 As shown...
Embodiment 3
[0075] Example 3 Nano-Selenium Carrying Amantadine Toxicity Assay (MTT) to MDCK Cells
[0076] MTT method was used to measure the effect of different drug components on MDCK cells after treatment: normal cell control group (Control), virus-infected cell group (Virus), virus-infected cell+nano selenium group (Virus+AM), virus-infected cell+King Kong Alkylamine group (Virus+SeNPs), virus-infected cells + nano-selenium-amantadine composite nano-drug carrier (Virus+Se@AM).
[0077] The steps are as follows: Digest the adherent cells with 0.25% trypsin for 3 minutes to make a single cell suspension, and place 4×10 cells in a 96-well culture plate. 4 Cells / well were seeded and placed in an incubator (37°C, 5% CO 2 ) after pre-cultivation for 24 hours, add H1N1 virus and incubate for 2 hours, then add 100 μL of medium containing different concentrations of drug groups, and continue to cultivate for 24 hours. Add 20 μL / well of MTT (5 mg / mL) to the culture plate, incubate at 37°C f...
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