Polypeptide realizing specific combination with mycoplasma pneumoniae positive serum and diagnostic kit

A technology of mycoplasma pneumoniae and diagnostic kits, applied in the direction of peptides, biological tests, and microbial-based methods, can solve the problems of cumbersome operations, high costs, and low specificity, and meet the requirements of low requirements, easy operation, and sensitivity and highly specific effects

Active Publication Date: 2018-10-23
CHENZHOU NO 1 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These previous studies were based on traditional protein expression and purification, which was cumbersome, expensive and low specificity

Method used

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  • Polypeptide realizing specific combination with mycoplasma pneumoniae positive serum and diagnostic kit
  • Polypeptide realizing specific combination with mycoplasma pneumoniae positive serum and diagnostic kit
  • Polypeptide realizing specific combination with mycoplasma pneumoniae positive serum and diagnostic kit

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Embodiment Construction

[0018] The present invention will be described in detail below in conjunction with specific experimental steps, but the protection scope of the present invention is not limited to the following examples.

[0019] In the following experiments, the purified Mycoplasma pneumoniae positive mixed serum was used as the target molecule, and the phage display random 7-peptide library was biopanned, and the phage that could specifically bind to the Mycoplasma pneumoniae positive mixed serum was screened, and the 7-peptide synthesized in solid phase As a coating antigen, the reaction of phages and foreign polypeptides with different clinical sera was detected to prove the application value of the phages and their polypeptides in the serological diagnosis of Mycoplasma pneumoniae.

[0020] Materials and methods: The positive mixed serum and negative mixed serum (from the First People's Hospital of Chenzhou City) of patients infected with Mycoplasma pneumoniae were saturated with (NH4) 2 ...

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Abstract

The invention provides a polypeptide realizing specific combination with mycoplasma pneumoniae positive serum. The amino acid sequence of the polypeptide is shown as SEQ ID NO: 1: L-P-Q-R-L-R-T. The invention also provides a mycoplasma pneumoniae infected serology diagnostic kit. The kit contains polypeptide realizing specific combination with mycoplasma pneumoniae positive serum. In addition, theinvention also provides a mycoplasma pneumoniae diagnostic kit for showing the polypeptide on the basis of polypeptide. The kit contains a core phage, wherein the core phage is a phage showing the polypeptide. The polypeptide and the corresponding core phage provided by the invention have the advantages that the sensitivity and the specificity degree are very high; good clinic diagnosis values are realized.

Description

technical field [0001] The invention provides a polypeptide capable of specifically binding to positive serum of Mycoplasma pneumoniae, a diagnostic kit based on the polypeptide, and further relates to a diagnostic kit for Mycoplasma pneumoniae based on a phage display polypeptide, which belongs to the field of biological preparations. Background technique [0002] Mycoplasma pneumoniae (Mycoplasma pneumoniae, MP) is a prokaryotic cell-type organism between viruses and bacteria. The pathological changes caused by its infection are mainly interstitial pneumonia, which accounts for about 1 / 2 of non-bacterial pneumonia in humans and animals. . MP is highly contagious, especially in children, and is the main cause of community-acquired pneumonia. Therefore, early diagnosis of MP pneumonia is extremely important. At present, Mycoplasma pneumoniae infection is mainly diagnosed by the isolation and culture of MP, serological detection, PCR detection and physical diagnosis. There i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12Q1/04G01N33/68C12R1/35
CPCC07K7/06C12Q1/04G01N33/68
Inventor 史文元曾焱华赵兰华滕文友徐玉娟江杨华李胜涛
Owner CHENZHOU NO 1 PEOPLES HOSPITAL
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