Skin repair solution containing stem cell extract and preparation method thereof

A technology of skin repair and extract, which is applied in the field of skin repair, can solve problems such as tingling sensation and troubles in use, and achieve the effect of good user experience, wide sources, and obvious repair effect

Inactive Publication Date: 2018-11-23
北京原肽干细胞医学研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Stem cell skin repair solution is a common skin repair product, but although the existing stem cell skin repair solution can repair the skin, there will be a slight tingling sensation when using it, espe

Method used

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  • Skin repair solution containing stem cell extract and preparation method thereof
  • Skin repair solution containing stem cell extract and preparation method thereof

Examples

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Example Embodiment

[0021] Example 1

[0022] A skin repair solution containing stem cell extract, comprising the following raw materials by weight: 1.6 parts of stem cell extract, 0.6 parts of sodium hyaluronate, 15.3 parts of mannitol, 3.2 parts of nicotinamide, 2.1 parts of betaine, and oligopeptide-3 4.8 parts, 2.2 parts of polydimethylsiloxane, 0.4 parts of azone, and 5 parts of Prunella vulgaris. The stem cell extracts are Huatong’s block fragments, digested with collagenase II-containing DMEM medium, and used after filtration. Subculture in DMEM medium with 8% fetal bovine serum FBS, then switch to serum-free DMEM medium for subculture, subculture once every 2 days, collect and pass 3 passages of cells for subsequent processing; digest cells with EDTA-containing DMEM medium and use Wash with PBS solution, then resuspend in serum-free DMEM medium, ultrasonically lyse the broken cells, centrifuge the broken product and take the supernatant; filter the supernatant with a filter with a pore size ...

Example Embodiment

[0028] Example 2

[0029] A skin repair solution containing stem cell extract, comprising the following parts by weight of raw materials: stem cell extract 2.1 parts, sodium hyaluronate 0.88 parts, mannitol 16.2 parts, nicotinamide 3.64-4.4 parts, betaine 2.57 parts, oligopeptides -3 5.52 parts, 2.75 parts of polydimethylsiloxane, 0.48 parts of azone, and 5.63 parts of Prunella vulgaris. The stem cell extract is the Wharton block fragments and digested with DMEM medium containing collagenase II. Subculture in DMEM medium with 10% fetal bovine serum FBS, then subculture with serum-free DMEM medium, subculture once every 2 days, collect and pass 5 passages of cells for subsequent processing; digest cells with DMEM medium containing EDTA , Washed with PBS solution, resuspended in serum-free DMEM medium, lysed the broken cells by ultrasonic wave, centrifuged the broken product, and took the supernatant; filtered the supernatant with a filter with a pore size of 5000 angstroms.

[0030...

Example Embodiment

[0035] Example 3

[0036] A skin repair solution containing stem cell extract, comprising the following parts by weight of raw materials: stem cell extract 2.85 parts, sodium hyaluronate 1.02 parts, mannitol 17.36 parts, nicotinamide 3.98 parts, betaine 3.12 parts, oligopeptide-3 6.76 parts, 3.04 parts of dimethicone, 0.66 parts of azone, and 6.28 parts of Prunella vulgaris. The stem cell extract is Huatong’s block fragments and digested with DMEM medium containing collagenase II, filtered and used with mass fraction Subculture in DMEM medium with 10% fetal bovine serum FBS, then switch to serum-free DMEM medium for subculture, subculture once every 2 days, collect and pass 4 passages of cells for subsequent processing; digest cells with EDTA-containing DMEM medium and use Wash with PBS solution, then resuspend in serum-free DMEM medium, ultrasonically lyse the broken cells, centrifuge the broken product and take the supernatant; filter the supernatant with a filter with a pore s...

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Abstract

The invention discloses a skin repair solution containing stem cell extract and a preparation method thereof. The skin repair solution is prepared from the following raw materials in parts by weight:1.6 to 3.6 parts of stem cell extract, 0.6 to 1.3 parts of sodium hyaluronate, 15.3 to 19.8 parts of mannitol, 3.2 to 4.4 parts of niacinamide, 2.1 to 3.7 parts of betaine, 4.8 to 8.5 parts of oligopeptide, 2.2 to 3.4 parts of polydimethylsiloxane, 0.4 to 0.9 part of azone, and 5 to 7.5 parts of spica prunellae. The skin repair solution has the advantages that the sources of the raw materials arewide; by adopting the active polypeptide and the stem cell extract, and compounding with other components, the multiple components can reach the synergistic function under the mutual action; the prepared finished product has obvious repair effect on the skin, any irritation feel and adverse reaction are avoided in the use process, the experience feel is good in use, and the application prospect isbroad.

Description

technical field [0001] The invention relates to the field of skin repair, in particular to a skin repair solution containing stem cell extract. Background technique [0002] The skin covers the surface of the whole body and is one of the largest organs in the human body. It has the functions of blocking the invasion of foreign bodies and pathogens, preventing the loss of body fluids, etc., and plays an important role in protecting the human body. It is thickest on the palms and soles of the feet, and thinnest on the armpits and face. The skin also has important physiological functions such as sensation, protection, and temperature regulation to maintain the opposition and unity between the body and the external environment. At the same time, the skin is an important part of the body's immune system. [0003] With the increase of age and the deterioration of the living environment, the metabolism of the skin decreases, the function of absorbing various beneficial elements de...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/73A61K8/67A61K8/44A61K8/64A61K8/9789A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/44A61K8/64A61K8/675A61K8/735A61K8/9789A61K8/99A61K2800/592A61Q19/00A61Q19/02A61Q19/08
Inventor 杨曦光刘思寒段秀芬
Owner 北京原肽干细胞医学研究院有限公司
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