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Freehand sectioning method of peony tuber tissues and application of freehand sectioning method

A technique for freehand slicing and rhizomes, which is applied in the field of freehand slicing of peony rhizomes to improve research efficiency, clear pictures, and solve harsh experimental conditions.

Inactive Publication Date: 2018-11-23
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Existing slicing methods cannot fully meet the above conditions, thus exhibiting certain deficiencies

Method used

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  • Freehand sectioning method of peony tuber tissues and application of freehand sectioning method
  • Freehand sectioning method of peony tuber tissues and application of freehand sectioning method
  • Freehand sectioning method of peony tuber tissues and application of freehand sectioning method

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Embodiment Construction

[0033] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. The following examples are used to illustrate the present invention, but are not used to limit the protection scope of the present invention, and the protection scope of the present invention is as the criterion with claims.

[0034] From the end of April to mid-May, observe and sample the underground fleshy roots, stems and rhizomes of peony, peony and peony plants planted in the Xiaotangshan Germplasm Resource Garden of the National Flower Engineering Center (Beijing, Changping District, Xiaotangshan Town) At this time, peony, Ito peony and peony are in the flowering stage.

[0035] Raise the whole peony plant with a stainless steel fork, remove the soil and debris around the fleshy roots and rhizomes, then select fleshy roots and rhizomes with a diameter of about 0.5-1cm, cut them off completely with a s...

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Abstract

The invention discloses a freehand sectioning method of peony tuber tissues and application of the freehand sectioning method. The freehand sectioning method comprises the following steps of acquiringclean and fresh peony tuber tissues, dewatering the peony tuber tissues until the water content is 5-10 percent, cutting the obtained tuber tissues with a blade in a direction perpendicular to a longitudinal axis to acquire a section with the thickness being 20-30 microns, putting the section in absolute ethyl alcohol to remove excessive moisture, and putting the treated section in distilled water for complete unfolding. For the application of the freehand sectioning method, the section is put under a microscope for observation and photographing, and photographing is finished within 15 minutes after sectioning is finished. The freehand sectioning method provided by the invention is simple to operate, high in sectioning efficiency and low in cost, can thoroughly solve the problems of severe experiment condition, complex operation process, use of a toxic reagent, relatively long time and relatively large cost of experiment investment and the like of the existing paraffin sectioning method, is simple and available in used tools and low in cost, does not use the toxic reagent, is environmentally friendly, is wide in application and has an important theoretical and practical significance.

Description

technical field [0001] The invention belongs to the technical field of plant tissue slicing, and in particular relates to a free-hand slicing method of peony rhizome tissue and an application thereof. Background technique [0002] China is one of the centers of origin of the genus Paeoniae in the world. It has a long history of cultivation and application in my country. Its flowers are large and beautiful, and most of them are famous medicinal ornamental plants. They are deeply loved by people in my country and the world. The fleshy root is the material storage center of Paeoniae genus, which not only provides a strong energy supply for the normal growth and development of the plant, but also is the main source for the extraction of its medicinal ingredients; the formation and development of the stem are directly related to the quality of the flowering state, It is the basis for the formation of ornamental characters, and has a direct impact on the quantity, quality and yiel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/06G01N1/28B26D3/28
CPCB26D3/28G01N1/06G01N1/2806
Inventor 于晓南张建军程堂仁张启翔
Owner BEIJING FORESTRY UNIVERSITY
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