Selective breeding method for radish cytoplasmic male sterile lines and maintainer lines thereof with high seed production yield
A technology of cytoplasmic sterile lines and cytoplasmic male sterility, applied in the field of genetic breeding, can solve the problems of shrinking planting area of local varieties, comprehensive traits such as comprehensive yield resistance, uniformity, and weak tolerance to extreme weather
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0044] This embodiment provides a method for breeding a cytoplasmic sterile line of radish with high seed production yield and its maintainer line. The breeding method includes the following steps:
[0045] 1.1 The female parent of the radish Ogura male sterile line CMS17-13 was crossed with the landrace radish fertile variety as the male parent to obtain F 1 substitute seeds;
[0046]1.2 Sowing F 1 generation seed, according to F 1 Anther development and the presence or absence of pollen in the generation plant are screened, and the maintainer male parent with maintenance performance is selected in the male parent;
[0047] 1.3 will F 1 Germination of generation seeds and maintainer male seeds; then vernalization at 0°C for 15 days;
[0048] 1.4 will F 1 The generation seeds and the male parent seeds of the maintainer line were sown in plug trays respectively, and the seedlings were raised in the solar greenhouse. 1 Generation male sterile line seedlings and maintainer ...
Embodiment 2
[0057] This embodiment provides a method for breeding a cytoplasmic sterile line of radish with high seed production yield and its maintainer line. The breeding method includes the following steps:
[0058] 1.1 The female parent of the radish Ogura male sterile line CMS17-13 was crossed with the landrace radish fertile variety as the male parent to obtain F 1 substitute seeds;
[0059] 1.2 Sowing F 1 generation seed, according to F 1 Anther development and the presence or absence of pollen in the generation plant are screened, and the maintainer male parent with maintenance performance is selected in the male parent;
[0060] 1.3 will F 1 Germination of generation seeds and maintainer male seeds; then vernalization at 4°C for 30 days;
[0061] 1.4 will F 1 The generation seeds and the male parent seeds of the maintainer line were sown in plug trays respectively, and the seedlings were raised in the solar greenhouse. 1 CMS seedlings and maintainer (male parent) seedlings;...
Embodiment 3
[0070] This embodiment provides a method for breeding a cytoplasmic sterile line of radish with high seed production yield and its maintainer line. The breeding method includes the following steps:
[0071] 1.1 The female parent of the radish Ogura male sterile line CMS17-13 was crossed with the landrace radish fertile variety as the male parent to obtain F 1 substitute seeds;
[0072] 1.2 Sowing F 1 generation seed, according to F 1 Anther development and the presence or absence of pollen in the generation plant are screened, and the maintainer male parent with maintenance performance is selected in the male parent;
[0073] 1.3 will F 1 Germination of generation seeds and maintainer male parent seeds; then vernalization at 6°C for 25 days;
[0074] 1.4 will F 1 The generation seeds and the maintainer (male parent) seeds were sown in plug trays respectively, and the seedlings were raised in a solar greenhouse. The seedling substrate was prepared by mixing peat and vermic...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More