Method for determining contents of chlorogenic acid, luteolin and apigenin in longleaf campanumoea root

A technology of luteolin and determination method, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of unfavorable medicinal materials and the quality of their formulations, limit the development and utilization of spider fruit, and cannot guarantee the safety and effectiveness of clinical medication, etc. question

Active Publication Date: 2018-11-27
贵州中医药大学
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Problems solved by technology

Through literature review and analysis, there are relatively few research reports on spider fruit medicinal materials, mainly focusing on its chemical composition and antioxidant activity, and there is still a gap in quality control, wh

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  • Method for determining contents of chlorogenic acid, luteolin and apigenin in longleaf campanumoea root
  • Method for determining contents of chlorogenic acid, luteolin and apigenin in longleaf campanumoea root
  • Method for determining contents of chlorogenic acid, luteolin and apigenin in longleaf campanumoea root

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Embodiment 1

[0083] A method for determining the content of chlorogenic acid, luteolin and apigenin in spider fruit medicinal materials:

[0084] Chromatographic conditions: Pntulips BP-C 18 Column (4.6mm×250mm, 2.5μm); mobile phase: mobile phase A is acetonitrile, mobile phase B is methanol, mobile phase C is 0.1% phosphoric acid aqueous solution, gradient elution; detection wavelength: 340nm; column temperature: 25°C; Flow rate: 1.0mL·min -1 ;Injection volume: 10μL; Gradient elution program: 0~5min, 2%~12%A, 3%~8%B, 95%~80%C; 5~15min, 12%~6% A, 8%~14% B, 80%~80%C; 15~20min, 6%~16%A, 14%~10%B, 80%~74%C; 20~25min, 16%~22% A, 10%~8% B, 74%~70%C; 25~35min, 22%~34%A, 8%~16%B, 70%~50%C; 35~40min, 34%~0% A, 16%~100%B, 50%~0%C; 40~45min, 0%A, 100%B, 0%C.

[0085] Preparation of chlorogenic acid reference substance stock solution: take chlorogenic acid reference substance, add 50% methanol to dissolve and make a solution with a mass concentration of 1.405 mg / mL;

[0086] Preparation of luteo...

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Abstract

The invention discloses a method for determining the contents of chlorogenic acid, luteolin and apigenin in longleaf campanumoea root. The method comprises the following steps: carrying out gradient elution, wherein chromatographic conditions are as follows: a chromatographic column is Pntulips Bp-C18(4.6mm*250mm and 2.5 microns), a flow phase A is acetonitrile, a flow phase B is methanol, a flowphase C is a 0.1% phosphoric acid water solution, the detection wavelength is 330nm-350nm, the column temperature is 20-30 DEG C, the flow speed is 0.8mL.min(-1)-1.2mL.min(-1), and the feeding quantity is 5-15 microliters; preparing a reference substance solution; preparing a testing sample solution; and carrying out determination by virtue of an HPLC method. By utilizing the HPLC method and optimizing an extraction method and the chromatographic conditions, a quantitative assay of multi-components by single-marker is established and is used for determining the contents of active components inthe longleaf campanumoea root, and a research basis is provided for the formulation of medicinal material quality standards, the reasonable development and utilization of resources and the like. Themethod is accurate, high in sensitivity and good in repeatability, the result is reliable, and basis is provided for the quality control and evaluation of the longleaf campanumoea root.

Description

technical field [0001] The invention relates to a content determination method of chlorogenic acid, luteolin and apigenin in spider fruit medicinal materials, belonging to the field of pharmaceutical technology. Background technique [0002] The multi-component and multi-target characteristics of traditional Chinese medicine determine that the quality control mode of traditional Chinese medicine must be multi-component quantification and multi-indicator monitoring. At present, the method of "one test and multiple evaluation" has been widely used in the quality control research of Chinese medicinal materials and traditional Chinese medicine preparations. Intrinsic functional relationship and proportional relationship between chemical components, determination of one component (the reference substance is cheap and easy to obtain), to realize the synchronous monitoring of multiple components (the reference substance is not available or difficult to obtain), making the quality e...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14G01N30/54G01N30/74
CPCG01N30/02G01N30/06G01N30/14G01N30/54G01N30/74G01N2030/027G01N2030/062G01N2030/146
Inventor 孙庆文刘梦鸽徐文芬潘国吉杨杭
Owner 贵州中医药大学
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