Method for tissue culture detoxified seedling raising of epiphyllum

A technology of epiphyllum and tissue culture, which is applied in the field of plant tissue culture and horticulture, can solve the problems of unsatisfactory quantity and quality, and achieve the effect of good disinfection effect, low pollution rate and simple process

Inactive Publication Date: 2018-11-30
LIANYUNGANG XIUJING LANDSCAPING ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The economic value of epiphyllum is more and more recognized by people, and the market demand is gradually increasing. Relying on traditional cutting seedlings cannot meet the actual production needs in terms of quantity and quality.

Method used

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  • Method for tissue culture detoxified seedling raising of epiphyllum
  • Method for tissue culture detoxified seedling raising of epiphyllum
  • Method for tissue culture detoxified seedling raising of epiphyllum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032]The method for seedling detoxification of epiphyllum tissue culture comprises the following steps:

[0033] (1) Selection of epiphyllum plants and cyclic heat treatment:

[0034] Select the epiphyllum plants that grow robustly and are free of diseases and insect pests, put them in the cultivation room, and cultivate them at 36-38°C and 2000-2200Lux light conditions for 10-12 hours a day, and then cultivate them at 22-25°C and dark conditions for 12-14 hours , and then returned to the environment of 36 ~ 38 ° C, and cultivated in this way for 33 days;

[0035] (2) Disinfection of explants and stripping of shoot tips:

[0036] Take the plant in step (1), cut off the 2cm-long young stem section with the growth point on the branch tip, first dip in a small amount of washing liquid and wash it gently by hand for 2-3 times, then rinse it with running water for 30-60 minutes, and then remove the stem The section was transferred to the ultra-clean workbench, firstly disinfecte...

Embodiment 2

[0044] The operation method is the same as that in Example 1, wherein the plan of carrying out cyclic heat treatment to the epiphyllum plants in step (1) is cancelled.

Embodiment 3

[0046] The method of operation is the same as in Example 1, wherein the method of disinfecting the epiphyllum explants in step (2) is changed to a conventional disinfection method, that is, first adopting a volume fraction of 75% alcohol for disinfection for 3 to 5 minutes, and then adopting a mass fraction of Sterilize with 0.1% mercuric chloride solution for 10 minutes, and finally blot the surface moisture with sterile filter paper; use a scalpel to cut off the stem segment 0.5 cm from the tip of the stem in an aseptic operating environment, and place it in 0.1% mercuric chloride solution for 20 seconds , and then sterilize it in 75% alcohol for 10 seconds.

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Abstract

The invention relates to the technical field of plant tissue culture and particularly provides a method for tissue culture detoxified seedling raising of epiphyllum. The method comprises the followingsteps: (1) selection and circulating heat treatment of epiphyllum plants; (2) disinfection and stem tip stripping of explants; (3) induced culture of calli; (4) differentiated culture of calli; and (5) virus detection. According to the method provided by the invention, the problem of the conventional epiphyllum propagating methods that the variety is deteriorated due to virus susceptiveness can be avoided, the survival rate of stem tips is high, the induced differentiation effect of the calli is good, and the detoxification rate is high, so that the success rate of detoxified seedling cultureof the epiphyllum can be greatly increased, and the meeting of market requirements is facilitated.

Description

technical field [0001] The invention relates to a method for cultivating detoxified seedlings of epiphyllum by tissue culture, and belongs to the technical fields of plant tissue culture and horticulture. Background technique [0002] Epiphyllum (scientific name: Epiphyllum oxypetalum (DC.) Haw), alias Qionghua and Moon Beauty, belong to the shrub-like woody plants of the family Cactaceae and the genus Epiphyllum. The main stem is thin cylindrical, the branches are flat and leaf-shaped, and the flowers are mostly white, large, funnel-shaped, and fragrant. Native to the tropical forests of Mexico and Central and South America, it likes warm, humid and semi-shady environments. It is now widely cultivated in various regions of the world, and is also commonly cultivated in various provinces and regions in China. Epiphyllum generally blooms in the evenings of summer and autumn, and lasts for a short time, generally 2 to 3 hours, so there is a saying of "flash in the pan". The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 韩佳佳孔令群李晶瑶
Owner LIANYUNGANG XIUJING LANDSCAPING ENG CO LTD
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