Model establishment and evaluation method for nephritic syndrome rats

A technology of nephrotic syndrome and rat models, applied in medical simulation, character and pattern recognition, special data processing applications, etc., can solve problems such as poor accuracy and low sensitivity, achieve strong specificity, reflect rationality and scientific Effect

Inactive Publication Date: 2018-11-30
SHANXI UNIV
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Problems solved by technology

[0004] In order to solve the technical problems of low sensitivity and poor accuracy in the existing construction and evaluation m...

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  • Model establishment and evaluation method for nephritic syndrome rats
  • Model establishment and evaluation method for nephritic syndrome rats
  • Model establishment and evaluation method for nephritic syndrome rats

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Experimental program
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Embodiment Construction

[0024] A construction method of nephrotic syndrome rat model, comprising the following steps:

[0025] (1) Rats were injected with 4 mg / kg of doxorubicin into the tail vein on the first day, and then 1 mg / kg of doxorubicin was injected into the tail vein on the eighth day to obtain a rat model of doxorubicin nephropathy;

[0026] (2) Multivariate statistical analysis method was used to characterize the metabolic profile, principal component analysis (PCA) was used to identify the pattern of the data, and the separation of the data profiles of each group was investigated. The specific method is to collect the urine of the rat model respectively in the 0th week, the 2nd week, the 4th week, and the 6th week of the rat model construction, and first collect the urine of the 0th week, the 2nd week, the 4th week, and the 6th week. The collected urine of the rat model was analyzed by nuclear magnetic resonance, and the results of the rat model were obtained. 1 H NMR spectrum; then th...

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Abstract

The invention belongs to the technical field of model establishment and evaluation methods with an aim to solve problems about low flexibility and poor accuracy in an existing model establishment andevaluation method for the nephritic syndrome rats and provides a model establishment and evaluation method for the nephritic syndrome rats. Changes of endogenous metabolites in the urine of terminal products are analyzed by metabolomics, all 1 HNMR spectra are processed through MestReNova software to acquire integral data, statistical analysis of the content of 12 biomarkers is combined with, thechanges of the mean value of the 12 biomarkers in the urine before and after modeling reflect the change trend of the urine metabolite content in the nephritic syndrome rats, and models of nephritic syndrome are evaluated in a targeted manner. Dynamic body contours before and after modeling is reflected comprehensively, flexibly and systematically, rationality and scientific nature of model reproduction are comprehensively reflected, the reliable evaluation method for new drug development and pharmacological research is provided, and the method is efficient, rapid, noninvasive and high in specificity.

Description

technical field [0001] The invention belongs to the technical field of model construction and evaluation methods, and in particular relates to a construction and evaluation method of a nephrotic syndrome rat model. Background technique [0002] Nephrotic syndrome is a syndrome of changes in the permeability of the glomerular filtration membrane due to various etiologies. A large amount of proteinuria is its main characteristic and cause. Nephrotic syndrome is a multiple refractory disease in children with a high incidence. Up to 2~10 / 100000, it is the second largest kidney disease after acute glomerulonephritis, seriously affecting human health. [0003] At present, the judgment of the success of nephrotic syndrome model replication is mainly based on 24-h urine protein quantification, renal histopathological examination and serum biochemical indicators. Serum SOD and MDA levels, renal tissue inflammatory factors, fibrosis factor levels, and renal podocyte structural protei...

Claims

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Application Information

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IPC IPC(8): G06F19/24G06F19/18G16H50/50G16H50/20G06K9/62
CPCG16H50/20G16H50/50G06F18/2135
Inventor 李爱平张王宁秦雪梅李震宇张丽增
Owner SHANXI UNIV
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